Prime Vaccination with Chitosan-Coated Phipps BCG and Boosting with CFP-PLGA against Tuberculosis in a Goat Model
Attempts to improve the immune response and efficacy of vaccines against tuberculosis in cattle, goats, and other animal species have been the focus of research in this field during the last two decades. Improving the vaccine efficacy is essential prior to running long-lasting and expensive field tr...
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MDPI AG
2021-04-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/11/4/1046 |
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| author | Yesenia Guadalupe Contreras-Magallanes Marina Durán-Aguilar Susana L. Sosa-Gallegos Ángel H. Álvarez Fátima A. Andrade-Santillán Isabel Bárcenas-Reyes Sara González-Ruíz Elba Rodríguez-Hernández Germinal J. Cantó-Alarcón Feliciano Milián-Suazo |
| author_facet | Yesenia Guadalupe Contreras-Magallanes Marina Durán-Aguilar Susana L. Sosa-Gallegos Ángel H. Álvarez Fátima A. Andrade-Santillán Isabel Bárcenas-Reyes Sara González-Ruíz Elba Rodríguez-Hernández Germinal J. Cantó-Alarcón Feliciano Milián-Suazo |
| author_sort | Yesenia Guadalupe Contreras-Magallanes |
| collection | DOAJ |
| description | Attempts to improve the immune response and efficacy of vaccines against tuberculosis in cattle, goats, and other animal species have been the focus of research in this field during the last two decades. Improving the vaccine efficacy is essential prior to running long-lasting and expensive field trials. Studies have shown that vaccine protocols utilizing boosting with proteins improve the vaccine efficacy. The use of polymers such as chitosan and PolyLactic-co-Glycolic Acid (PLGA) improves the immune response against different diseases by improving the interaction of antigens with the cellular immune system and modulating the host immune response. This study shows that the prime BCG vaccination, boosted with a culture filtrate protein (CFP), alone or in combination with chitosan and PolyLactic-co-Glycolic Acid (PLGA), have the potential to reduce tuberculosis (TB) dissemination by reducing the number of animals with lesions, the number of lesions per animal, and the size of the lesions in vaccinated animals, compared with those not vaccinated or those vaccinated with BCG alone. The vaccinated groups showed significantly higher Interferon-γ levels in the blood compared to the control, nonvaccinated group after vaccination, after boosting, and after the challenge with the wild-type <i>Mycobacterium bovis</i> strain. |
| first_indexed | 2024-03-10T12:31:09Z |
| format | Article |
| id | doaj.art-a5d0066220d44a7283694c0210b6a33c |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T12:31:09Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-a5d0066220d44a7283694c0210b6a33c2023-11-21T14:38:18ZengMDPI AGAnimals2076-26152021-04-01114104610.3390/ani11041046Prime Vaccination with Chitosan-Coated Phipps BCG and Boosting with CFP-PLGA against Tuberculosis in a Goat ModelYesenia Guadalupe Contreras-Magallanes0Marina Durán-Aguilar1Susana L. Sosa-Gallegos2Ángel H. Álvarez3Fátima A. Andrade-Santillán4Isabel Bárcenas-Reyes5Sara González-Ruíz6Elba Rodríguez-Hernández7Germinal J. Cantó-Alarcón8Feliciano Milián-Suazo9Doctorado en Ciencias Biológicas, Universidad Autónoma de Querétaro, Avenida de las Ciencias S/N, Juriquilla, Delegación Santa Rosa Jáuregui, 76230 Querétaro, MexicoFacultad de Ciencias Naturales, Universidad Autónoma de Querétaro, Avenida de las Ciencias S/N, Juriquilla, Delegación Santa Rosa Jáuregui, 76239 Querétaro, MexicoFacultad de Ciencias Naturales, Universidad Autónoma de Querétaro, Avenida de las Ciencias S/N, Juriquilla, Delegación Santa Rosa Jáuregui, 76239 Querétaro, MexicoCentro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, Av. Normalistas 800, Col. Colinas de la Normal, 44370 Guadalajara, MexicoMaestría en Salud y Producción Animal Sustentable, Universidad Autónoma de Querétaro, Avenida de las Ciencias S/N Juriquilla, Delegación Santa Rosa Jáuregui, 78230 Querétaro, MexicoFacultad de Ciencias Naturales, Universidad Autónoma de Querétaro, Avenida de las Ciencias S/N, Juriquilla, Delegación Santa Rosa Jáuregui, 76239 Querétaro, MexicoFacultad de Ciencias Naturales, Universidad Autónoma de Querétaro, Avenida de las Ciencias S/N, Juriquilla, Delegación Santa Rosa Jáuregui, 76239 Querétaro, MexicoCentro Nacional de Investigación Disciplinaria en Fisiología y Mejoramiento Animal, INIFAP, SAGARPA, Km. 1 Carretera a Colón, Ajuchitlan, 76280 Colón, MexicoFacultad de Ciencias Naturales, Universidad Autónoma de Querétaro, Avenida de las Ciencias S/N, Juriquilla, Delegación Santa Rosa Jáuregui, 76239 Querétaro, MexicoFacultad de Ciencias Naturales, Universidad Autónoma de Querétaro, Avenida de las Ciencias S/N, Juriquilla, Delegación Santa Rosa Jáuregui, 76239 Querétaro, MexicoAttempts to improve the immune response and efficacy of vaccines against tuberculosis in cattle, goats, and other animal species have been the focus of research in this field during the last two decades. Improving the vaccine efficacy is essential prior to running long-lasting and expensive field trials. Studies have shown that vaccine protocols utilizing boosting with proteins improve the vaccine efficacy. The use of polymers such as chitosan and PolyLactic-co-Glycolic Acid (PLGA) improves the immune response against different diseases by improving the interaction of antigens with the cellular immune system and modulating the host immune response. This study shows that the prime BCG vaccination, boosted with a culture filtrate protein (CFP), alone or in combination with chitosan and PolyLactic-co-Glycolic Acid (PLGA), have the potential to reduce tuberculosis (TB) dissemination by reducing the number of animals with lesions, the number of lesions per animal, and the size of the lesions in vaccinated animals, compared with those not vaccinated or those vaccinated with BCG alone. The vaccinated groups showed significantly higher Interferon-γ levels in the blood compared to the control, nonvaccinated group after vaccination, after boosting, and after the challenge with the wild-type <i>Mycobacterium bovis</i> strain.https://www.mdpi.com/2076-2615/11/4/1046<i>Mycobacterium bovis</i>BCG vaccinechitosanpoly (lactic-co-glycolic acid)cattlegoats |
| spellingShingle | Yesenia Guadalupe Contreras-Magallanes Marina Durán-Aguilar Susana L. Sosa-Gallegos Ángel H. Álvarez Fátima A. Andrade-Santillán Isabel Bárcenas-Reyes Sara González-Ruíz Elba Rodríguez-Hernández Germinal J. Cantó-Alarcón Feliciano Milián-Suazo Prime Vaccination with Chitosan-Coated Phipps BCG and Boosting with CFP-PLGA against Tuberculosis in a Goat Model Animals <i>Mycobacterium bovis</i> BCG vaccine chitosan poly (lactic-co-glycolic acid) cattle goats |
| title | Prime Vaccination with Chitosan-Coated Phipps BCG and Boosting with CFP-PLGA against Tuberculosis in a Goat Model |
| title_full | Prime Vaccination with Chitosan-Coated Phipps BCG and Boosting with CFP-PLGA against Tuberculosis in a Goat Model |
| title_fullStr | Prime Vaccination with Chitosan-Coated Phipps BCG and Boosting with CFP-PLGA against Tuberculosis in a Goat Model |
| title_full_unstemmed | Prime Vaccination with Chitosan-Coated Phipps BCG and Boosting with CFP-PLGA against Tuberculosis in a Goat Model |
| title_short | Prime Vaccination with Chitosan-Coated Phipps BCG and Boosting with CFP-PLGA against Tuberculosis in a Goat Model |
| title_sort | prime vaccination with chitosan coated phipps bcg and boosting with cfp plga against tuberculosis in a goat model |
| topic | <i>Mycobacterium bovis</i> BCG vaccine chitosan poly (lactic-co-glycolic acid) cattle goats |
| url | https://www.mdpi.com/2076-2615/11/4/1046 |
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