Limitations of PCR detection of filarial DNA in human stools from subjects non-infected with soil-transmitted helminths
The standard techniques for diagnosis of human filariasis are the microscopic examination of blood smears or skin biopsies, which are relatively invasive and poorly sensitive at low levels of infection. Recently, filarial DNA has been detected in fecal samples from non-human primates in Central Afri...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
EDP Sciences
2021-01-01
|
| Series: | Parasite |
| Subjects: | |
| Online Access: | https://www.parasite-journal.org/articles/parasite/full_html/2021/01/parasite200212/parasite200212.html |
| _version_ | 1797428401805459456 |
|---|---|
| author | Doret Maxime P. M. Nana-Djeunga Hugues C. Nzune-Toche Narcisse Pion Sébastien D. S. Chesnais Cédric B. Boussinesq Michel Kamgno Joseph Varlet-Marie Emmanuelle Locatelli Sabrina |
| author_facet | Doret Maxime P. M. Nana-Djeunga Hugues C. Nzune-Toche Narcisse Pion Sébastien D. S. Chesnais Cédric B. Boussinesq Michel Kamgno Joseph Varlet-Marie Emmanuelle Locatelli Sabrina |
| author_sort | Doret Maxime P. M. |
| collection | DOAJ |
| description | The standard techniques for diagnosis of human filariasis are the microscopic examination of blood smears or skin biopsies, which are relatively invasive and poorly sensitive at low levels of infection. Recently, filarial DNA has been detected in fecal samples from non-human primates in Central Africa. The aim of this study was to demonstrate proof-of-concept of a non-invasive molecular diagnosis technique for human filariasis by targeting fragments of 12S rDNA, Cox1, ITS1 and LL20-15kDa ladder antigen-gene by conventional PCR in DNA extracted from stool samples of 52 people infected with Mansonella perstans and/or Loa loa. Of these, 10 patients were infected with soil-transmitted helminths (Trichuris trichiura and/or Ascaris lumbricoides), and none were positive for Necator americanus. Interestingly, no filarial gene fragments were detected in the stools of any of the 52 patients. Future studies should evaluate whether a co-infection with soil-transmitted helminths causing gastrointestinal bleeding and likely allowing (micro)filaria exit into the digestive tract, may facilitate the molecular detection of filarial DNA fragments in stool samples. |
| first_indexed | 2024-03-09T08:58:22Z |
| format | Article |
| id | doaj.art-a5d4ed4c837f4601a2bb3c73427966a6 |
| institution | Directory Open Access Journal |
| issn | 1776-1042 |
| language | English |
| last_indexed | 2024-03-09T08:58:22Z |
| publishDate | 2021-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | Parasite |
| spelling | doaj.art-a5d4ed4c837f4601a2bb3c73427966a62023-12-02T12:38:25ZengEDP SciencesParasite1776-10422021-01-01284710.1051/parasite/2021046parasite200212Limitations of PCR detection of filarial DNA in human stools from subjects non-infected with soil-transmitted helminthsDoret Maxime P. M.0https://orcid.org/0000-0001-8312-3353Nana-Djeunga Hugues C.1https://orcid.org/0000-0002-4496-6048Nzune-Toche Narcisse2https://orcid.org/0000-0002-8875-7088Pion Sébastien D. S.3https://orcid.org/0000-0002-9468-7020Chesnais Cédric B.4https://orcid.org/0000-0002-8779-977XBoussinesq Michel5https://orcid.org/0000-0001-6312-0681Kamgno Josephhttps://orcid.org/0000-0003-1572-3490Varlet-Marie Emmanuelle6Locatelli Sabrina7https://orcid.org/0000-0002-1133-0561Institut de Recherche pour le Développement (IRD), UMI 233-INSERM U1175-University of MontpellierCentre for Research on Filariasis and Other Tropical Diseases (CRFilMT)Centre for Research on Filariasis and Other Tropical Diseases (CRFilMT)Institut de Recherche pour le Développement (IRD), UMI 233-INSERM U1175-University of MontpellierInstitut de Recherche pour le Développement (IRD), UMI 233-INSERM U1175-University of MontpellierInstitut de Recherche pour le Développement (IRD), UMI 233-INSERM U1175-University of MontpellierDepartment of Parasitology–Mycology, Centre Hospitalier Universitaire (CHU) of Montpellier, University of MontpellierInstitut de Recherche pour le Développement (IRD), UMI 233-INSERM U1175-University of MontpellierThe standard techniques for diagnosis of human filariasis are the microscopic examination of blood smears or skin biopsies, which are relatively invasive and poorly sensitive at low levels of infection. Recently, filarial DNA has been detected in fecal samples from non-human primates in Central Africa. The aim of this study was to demonstrate proof-of-concept of a non-invasive molecular diagnosis technique for human filariasis by targeting fragments of 12S rDNA, Cox1, ITS1 and LL20-15kDa ladder antigen-gene by conventional PCR in DNA extracted from stool samples of 52 people infected with Mansonella perstans and/or Loa loa. Of these, 10 patients were infected with soil-transmitted helminths (Trichuris trichiura and/or Ascaris lumbricoides), and none were positive for Necator americanus. Interestingly, no filarial gene fragments were detected in the stools of any of the 52 patients. Future studies should evaluate whether a co-infection with soil-transmitted helminths causing gastrointestinal bleeding and likely allowing (micro)filaria exit into the digestive tract, may facilitate the molecular detection of filarial DNA fragments in stool samples.https://www.parasite-journal.org/articles/parasite/full_html/2021/01/parasite200212/parasite200212.htmlmansonella perstansloa loastool samplepcrcameroon |
| spellingShingle | Doret Maxime P. M. Nana-Djeunga Hugues C. Nzune-Toche Narcisse Pion Sébastien D. S. Chesnais Cédric B. Boussinesq Michel Kamgno Joseph Varlet-Marie Emmanuelle Locatelli Sabrina Limitations of PCR detection of filarial DNA in human stools from subjects non-infected with soil-transmitted helminths Parasite mansonella perstans loa loa stool sample pcr cameroon |
| title | Limitations of PCR detection of filarial DNA in human stools from subjects non-infected with soil-transmitted helminths |
| title_full | Limitations of PCR detection of filarial DNA in human stools from subjects non-infected with soil-transmitted helminths |
| title_fullStr | Limitations of PCR detection of filarial DNA in human stools from subjects non-infected with soil-transmitted helminths |
| title_full_unstemmed | Limitations of PCR detection of filarial DNA in human stools from subjects non-infected with soil-transmitted helminths |
| title_short | Limitations of PCR detection of filarial DNA in human stools from subjects non-infected with soil-transmitted helminths |
| title_sort | limitations of pcr detection of filarial dna in human stools from subjects non infected with soil transmitted helminths |
| topic | mansonella perstans loa loa stool sample pcr cameroon |
| url | https://www.parasite-journal.org/articles/parasite/full_html/2021/01/parasite200212/parasite200212.html |
| work_keys_str_mv | AT doretmaximepm limitationsofpcrdetectionoffilarialdnainhumanstoolsfromsubjectsnoninfectedwithsoiltransmittedhelminths AT nanadjeungahuguesc limitationsofpcrdetectionoffilarialdnainhumanstoolsfromsubjectsnoninfectedwithsoiltransmittedhelminths AT nzunetochenarcisse limitationsofpcrdetectionoffilarialdnainhumanstoolsfromsubjectsnoninfectedwithsoiltransmittedhelminths AT pionsebastiends limitationsofpcrdetectionoffilarialdnainhumanstoolsfromsubjectsnoninfectedwithsoiltransmittedhelminths AT chesnaiscedricb limitationsofpcrdetectionoffilarialdnainhumanstoolsfromsubjectsnoninfectedwithsoiltransmittedhelminths AT boussinesqmichel limitationsofpcrdetectionoffilarialdnainhumanstoolsfromsubjectsnoninfectedwithsoiltransmittedhelminths AT kamgnojoseph limitationsofpcrdetectionoffilarialdnainhumanstoolsfromsubjectsnoninfectedwithsoiltransmittedhelminths AT varletmarieemmanuelle limitationsofpcrdetectionoffilarialdnainhumanstoolsfromsubjectsnoninfectedwithsoiltransmittedhelminths AT locatellisabrina limitationsofpcrdetectionoffilarialdnainhumanstoolsfromsubjectsnoninfectedwithsoiltransmittedhelminths |