Twist1 Suppresses Cementoblast Differentiation
The transcription factor Twist1 is known to be closely associated with the formation of bone by mesenchymal stem cells and osteoblasts; however, the role of Twist1 in cementogenesis has not yet been determined. This study was undertaken to elucidate the roles of Twist1 in cementoblast differentiatio...
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-10-01
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| Series: | Dentistry Journal |
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| Online Access: | http://www.mdpi.com/2304-6767/6/4/57 |
| _version_ | 1811183815008190464 |
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| author | Jung-Sun Moon Seong-Duk Kim Hyun-Mi Ko Young-Jun Kim Sun-Hun Kim Min-Seok Kim |
| author_facet | Jung-Sun Moon Seong-Duk Kim Hyun-Mi Ko Young-Jun Kim Sun-Hun Kim Min-Seok Kim |
| author_sort | Jung-Sun Moon |
| collection | DOAJ |
| description | The transcription factor Twist1 is known to be closely associated with the formation of bone by mesenchymal stem cells and osteoblasts; however, the role of Twist1 in cementogenesis has not yet been determined. This study was undertaken to elucidate the roles of Twist1 in cementoblast differentiation by means of the gain- or loss-of-function method. We used alkaline phosphatase (ALP) and alizarin red S staining and quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) to determine whether the forced transient expression or knock-down of Twist1 in a mouse cementoblast cell line, OCCM-30, could affect cementogenic differentiation. Silencing Twist1 with small interference RNA (siRNA) enhanced the formation of mineralized tissue. The expression of several cementogenesis markers, such as bone sialoprotein (BSP), osteopontin (OPN), dentin matrix protein1 (DMP1), and dentin sialophosphoprotein (DSPP) mRNA, were upregulated. Transient Twist1 overexpression in OCCM-30 consistently suppressed mineralization capacity and downregulated the differentiation markers. These results suggest that the Twist1 transcription factor may play a role in regulating cementoblast differentiation. |
| first_indexed | 2024-04-11T13:04:01Z |
| format | Article |
| id | doaj.art-a5d50a045b0340ac935a4f973ddfa12c |
| institution | Directory Open Access Journal |
| issn | 2304-6767 |
| language | English |
| last_indexed | 2024-04-11T13:04:01Z |
| publishDate | 2018-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Dentistry Journal |
| spelling | doaj.art-a5d50a045b0340ac935a4f973ddfa12c2022-12-22T04:22:51ZengMDPI AGDentistry Journal2304-67672018-10-01645710.3390/dj6040057dj6040057Twist1 Suppresses Cementoblast DifferentiationJung-Sun Moon0Seong-Duk Kim1Hyun-Mi Ko2Young-Jun Kim3Sun-Hun Kim4Min-Seok Kim5Dental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju 61186, KoreaDental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju 61186, KoreaDental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju 61186, KoreaDental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju 61186, KoreaDental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju 61186, KoreaDental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju 61186, KoreaThe transcription factor Twist1 is known to be closely associated with the formation of bone by mesenchymal stem cells and osteoblasts; however, the role of Twist1 in cementogenesis has not yet been determined. This study was undertaken to elucidate the roles of Twist1 in cementoblast differentiation by means of the gain- or loss-of-function method. We used alkaline phosphatase (ALP) and alizarin red S staining and quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) to determine whether the forced transient expression or knock-down of Twist1 in a mouse cementoblast cell line, OCCM-30, could affect cementogenic differentiation. Silencing Twist1 with small interference RNA (siRNA) enhanced the formation of mineralized tissue. The expression of several cementogenesis markers, such as bone sialoprotein (BSP), osteopontin (OPN), dentin matrix protein1 (DMP1), and dentin sialophosphoprotein (DSPP) mRNA, were upregulated. Transient Twist1 overexpression in OCCM-30 consistently suppressed mineralization capacity and downregulated the differentiation markers. These results suggest that the Twist1 transcription factor may play a role in regulating cementoblast differentiation.http://www.mdpi.com/2304-6767/6/4/57Twist1cementoblast differentiationOCCM-30 |
| spellingShingle | Jung-Sun Moon Seong-Duk Kim Hyun-Mi Ko Young-Jun Kim Sun-Hun Kim Min-Seok Kim Twist1 Suppresses Cementoblast Differentiation Dentistry Journal Twist1 cementoblast differentiation OCCM-30 |
| title | Twist1 Suppresses Cementoblast Differentiation |
| title_full | Twist1 Suppresses Cementoblast Differentiation |
| title_fullStr | Twist1 Suppresses Cementoblast Differentiation |
| title_full_unstemmed | Twist1 Suppresses Cementoblast Differentiation |
| title_short | Twist1 Suppresses Cementoblast Differentiation |
| title_sort | twist1 suppresses cementoblast differentiation |
| topic | Twist1 cementoblast differentiation OCCM-30 |
| url | http://www.mdpi.com/2304-6767/6/4/57 |
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