Drought Shifts Sorghum Root Metabolite and Microbiome Profiles and Enriches for Pipecolic Acid
Plant-associated microbial communities shift in composition as a result of environmental perturbations, such as drought. It has been shown that Actinobacteria are enriched in plant roots and rhizospheres during drought stress. However, the correlations between microbiome dynamics and plant response...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2023-12-01
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| Series: | Phytobiomes Journal |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/PBIOMES-02-23-0011-R |
| _version_ | 1827330575054143488 |
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| author | Daniel F. Caddell Dean Pettinga Katherine Louie Benjamin P. Bowen Julie A. Sievert Joy Hollingsworth Rebeckah Rubanowitz Jeffery Dahlberg Elizabeth Purdom Trent Northen Devin Coleman-Derr |
| author_facet | Daniel F. Caddell Dean Pettinga Katherine Louie Benjamin P. Bowen Julie A. Sievert Joy Hollingsworth Rebeckah Rubanowitz Jeffery Dahlberg Elizabeth Purdom Trent Northen Devin Coleman-Derr |
| author_sort | Daniel F. Caddell |
| collection | DOAJ |
| description | Plant-associated microbial communities shift in composition as a result of environmental perturbations, such as drought. It has been shown that Actinobacteria are enriched in plant roots and rhizospheres during drought stress. However, the correlations between microbiome dynamics and plant response to drought are poorly understood. Here we apply a combination of bacterial community composition analysis and plant metabolite profiling in Sorghum bicolor roots, rhizospheres, and soil during drought and drought recovery to investigate potential contributions of host metabolism to shifts in bacterial composition. Our results provide a detailed view of metabolic shifts across the plant root during drought and show that the response to rewatering differs between root and soil; additionally, we identify drought-responsive metabolites that are highly correlated with the observed changes in Actinobacteria abundance. Furthermore, we find that pipecolic acid is a drought-enriched metabolite in sorghum roots, and that exogenous application of pipecolic acid inhibits root growth. Finally, we show that this activity functions independent of the systemic acquired resistance pathway and has the potential to impact Actinobacterial taxa within the root microbiome. |
| first_indexed | 2024-03-07T16:14:09Z |
| format | Article |
| id | doaj.art-a5d53d77dc53466aac49cd2ff4b798f1 |
| institution | Directory Open Access Journal |
| issn | 2471-2906 |
| language | English |
| last_indexed | 2024-03-07T16:14:09Z |
| publishDate | 2023-12-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Phytobiomes Journal |
| spelling | doaj.art-a5d53d77dc53466aac49cd2ff4b798f12024-03-04T15:24:27ZengThe American Phytopathological SocietyPhytobiomes Journal2471-29062023-12-017444946310.1094/PBIOMES-02-23-0011-RDrought Shifts Sorghum Root Metabolite and Microbiome Profiles and Enriches for Pipecolic AcidDaniel F. Caddell0Dean Pettinga1Katherine Louie2Benjamin P. Bowen3Julie A. Sievert4Joy Hollingsworth5Rebeckah Rubanowitz6Jeffery Dahlberg7Elizabeth Purdom8Trent Northen9Devin Coleman-Derr10Plant Gene Expression Center, U.S. Department of Agriculture-Agricultural Research Service, Albany, CA 94710Plant and Microbial Biology Department, University of California Berkeley, Berkeley, CA 94720Lawrence Berkeley National Laboratory, Berkeley, CA 94720Lawrence Berkeley National Laboratory, Berkeley, CA 94720Kearney Agricultural Research and Extension Center, Parlier, CA 93648Kearney Agricultural Research and Extension Center, Parlier, CA 93648Plant and Microbial Biology Department, University of California Berkeley, Berkeley, CA 94720Kearney Agricultural Research and Extension Center, Parlier, CA 93648Department of Statistics, University of California Berkeley, Berkeley, CA 94720Lawrence Berkeley National Laboratory, Berkeley, CA 94720Plant Gene Expression Center, U.S. Department of Agriculture-Agricultural Research Service, Albany, CA 94710Plant-associated microbial communities shift in composition as a result of environmental perturbations, such as drought. It has been shown that Actinobacteria are enriched in plant roots and rhizospheres during drought stress. However, the correlations between microbiome dynamics and plant response to drought are poorly understood. Here we apply a combination of bacterial community composition analysis and plant metabolite profiling in Sorghum bicolor roots, rhizospheres, and soil during drought and drought recovery to investigate potential contributions of host metabolism to shifts in bacterial composition. Our results provide a detailed view of metabolic shifts across the plant root during drought and show that the response to rewatering differs between root and soil; additionally, we identify drought-responsive metabolites that are highly correlated with the observed changes in Actinobacteria abundance. Furthermore, we find that pipecolic acid is a drought-enriched metabolite in sorghum roots, and that exogenous application of pipecolic acid inhibits root growth. Finally, we show that this activity functions independent of the systemic acquired resistance pathway and has the potential to impact Actinobacterial taxa within the root microbiome.https://apsjournals.apsnet.org/doi/10.1094/PBIOMES-02-23-0011-R16S rRNAamplicon sequencingdroughtmetabolomicsmicrobiomepipecolic acid |
| spellingShingle | Daniel F. Caddell Dean Pettinga Katherine Louie Benjamin P. Bowen Julie A. Sievert Joy Hollingsworth Rebeckah Rubanowitz Jeffery Dahlberg Elizabeth Purdom Trent Northen Devin Coleman-Derr Drought Shifts Sorghum Root Metabolite and Microbiome Profiles and Enriches for Pipecolic Acid Phytobiomes Journal 16S rRNA amplicon sequencing drought metabolomics microbiome pipecolic acid |
| title | Drought Shifts Sorghum Root Metabolite and Microbiome Profiles and Enriches for Pipecolic Acid |
| title_full | Drought Shifts Sorghum Root Metabolite and Microbiome Profiles and Enriches for Pipecolic Acid |
| title_fullStr | Drought Shifts Sorghum Root Metabolite and Microbiome Profiles and Enriches for Pipecolic Acid |
| title_full_unstemmed | Drought Shifts Sorghum Root Metabolite and Microbiome Profiles and Enriches for Pipecolic Acid |
| title_short | Drought Shifts Sorghum Root Metabolite and Microbiome Profiles and Enriches for Pipecolic Acid |
| title_sort | drought shifts sorghum root metabolite and microbiome profiles and enriches for pipecolic acid |
| topic | 16S rRNA amplicon sequencing drought metabolomics microbiome pipecolic acid |
| url | https://apsjournals.apsnet.org/doi/10.1094/PBIOMES-02-23-0011-R |
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