A small amount of cyclooxygenase 2 (COX2) is constitutively expressed in platelets
Cyclooxygenase (COX) is the rate-limiting enzyme in conversion of arachidonic acid to prostanoids, and has two isoforms, COX1 and COX2, which share ~65% amino acid homology. COX1 is universally expressed in many cell types including platelets; however, expression of COX2 is known to be more limited....
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2017-01-01
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| Series: | Platelets |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09537104.2016.1203406 |
| _version_ | 1797684347351859200 |
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| author | Qianghua Hu Min Soon Cho Perumal Thiagarajan Fleur M. Aung Anil K. Sood Vahid Afshar-Kharghan |
| author_facet | Qianghua Hu Min Soon Cho Perumal Thiagarajan Fleur M. Aung Anil K. Sood Vahid Afshar-Kharghan |
| author_sort | Qianghua Hu |
| collection | DOAJ |
| description | Cyclooxygenase (COX) is the rate-limiting enzyme in conversion of arachidonic acid to prostanoids, and has two isoforms, COX1 and COX2, which share ~65% amino acid homology. COX1 is universally expressed in many cell types including platelets; however, expression of COX2 is known to be more limited. We examined expression of COX2 mRNA and protein in platelets and platelet-derived microparticles (MPs); using quantitative RT-PCR, immunostaining, and Western blotting. We have detected a significant amount of COX2 in platelets, both at mRNA and protein levels. We found that COX1/COX2 mRNA and protein ratios in platelets were 370:1 and 17:1, respectively. Expression level of COX2 in platelets was less than COX1, but comparable to the expression of COX2 in malignant epithelial cells. Considering the important role of COX2 in tumorigenesis and thrombosis, and the large number of circulating platelets, we propose that platelet COX2 may play an important role in physiologic and pathologic conditions. |
| first_indexed | 2024-03-12T00:28:25Z |
| format | Article |
| id | doaj.art-a5d7378fa1af4e6d9be6b9e502da4767 |
| institution | Directory Open Access Journal |
| issn | 0953-7104 1369-1635 |
| language | English |
| last_indexed | 2024-03-12T00:28:25Z |
| publishDate | 2017-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Platelets |
| spelling | doaj.art-a5d7378fa1af4e6d9be6b9e502da47672023-09-15T10:26:28ZengTaylor & Francis GroupPlatelets0953-71041369-16352017-01-012819910210.1080/09537104.2016.12034061203406A small amount of cyclooxygenase 2 (COX2) is constitutively expressed in plateletsQianghua Hu0Min Soon Cho1Perumal Thiagarajan2Fleur M. Aung3Anil K. Sood4Vahid Afshar-Kharghan5The University of Texas M. D. Anderson Cancer CenterThe University of Texas M. D. Anderson Cancer CenterCenter for Translational Research on Inflammatory Diseases, Michael E. DeBakey Veterans Affairs Medical Center, Baylor College of MedicineThe University of Texas M. D. Anderson Cancer CenterThe University of Texas M. D. Anderson Cancer CenterThe University of Texas M. D. Anderson Cancer CenterCyclooxygenase (COX) is the rate-limiting enzyme in conversion of arachidonic acid to prostanoids, and has two isoforms, COX1 and COX2, which share ~65% amino acid homology. COX1 is universally expressed in many cell types including platelets; however, expression of COX2 is known to be more limited. We examined expression of COX2 mRNA and protein in platelets and platelet-derived microparticles (MPs); using quantitative RT-PCR, immunostaining, and Western blotting. We have detected a significant amount of COX2 in platelets, both at mRNA and protein levels. We found that COX1/COX2 mRNA and protein ratios in platelets were 370:1 and 17:1, respectively. Expression level of COX2 in platelets was less than COX1, but comparable to the expression of COX2 in malignant epithelial cells. Considering the important role of COX2 in tumorigenesis and thrombosis, and the large number of circulating platelets, we propose that platelet COX2 may play an important role in physiologic and pathologic conditions.http://dx.doi.org/10.1080/09537104.2016.1203406cox2plateletscox1cox2expression |
| spellingShingle | Qianghua Hu Min Soon Cho Perumal Thiagarajan Fleur M. Aung Anil K. Sood Vahid Afshar-Kharghan A small amount of cyclooxygenase 2 (COX2) is constitutively expressed in platelets Platelets cox2 platelets cox1cox2 expression |
| title | A small amount of cyclooxygenase 2 (COX2) is constitutively expressed in platelets |
| title_full | A small amount of cyclooxygenase 2 (COX2) is constitutively expressed in platelets |
| title_fullStr | A small amount of cyclooxygenase 2 (COX2) is constitutively expressed in platelets |
| title_full_unstemmed | A small amount of cyclooxygenase 2 (COX2) is constitutively expressed in platelets |
| title_short | A small amount of cyclooxygenase 2 (COX2) is constitutively expressed in platelets |
| title_sort | small amount of cyclooxygenase 2 cox2 is constitutively expressed in platelets |
| topic | cox2 platelets cox1cox2 expression |
| url | http://dx.doi.org/10.1080/09537104.2016.1203406 |
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