Restoring specific lactobacilli levels decreases inflammation and muscle atrophy markers in an acute leukemia mouse model.
The gut microbiota has recently been proposed as a novel component in the regulation of host homeostasis and immunity. We have assessed for the first time the role of the gut microbiota in a mouse model of leukemia (transplantation of BaF3 cells containing ectopic expression of Bcr-Abl), characteriz...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3384645?pdf=render |
| _version_ | 1819276903243055104 |
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| author | Laure B Bindels Raphaël Beck Olivier Schakman Jennifer C Martin Fabienne De Backer Florence M Sohet Evelyne M Dewulf Barbara D Pachikian Audrey M Neyrinck Jean-Paul Thissen Julien Verrax Pedro Buc Calderon Bruno Pot Corinne Grangette Patrice D Cani Karen P Scott Nathalie M Delzenne |
| author_facet | Laure B Bindels Raphaël Beck Olivier Schakman Jennifer C Martin Fabienne De Backer Florence M Sohet Evelyne M Dewulf Barbara D Pachikian Audrey M Neyrinck Jean-Paul Thissen Julien Verrax Pedro Buc Calderon Bruno Pot Corinne Grangette Patrice D Cani Karen P Scott Nathalie M Delzenne |
| author_sort | Laure B Bindels |
| collection | DOAJ |
| description | The gut microbiota has recently been proposed as a novel component in the regulation of host homeostasis and immunity. We have assessed for the first time the role of the gut microbiota in a mouse model of leukemia (transplantation of BaF3 cells containing ectopic expression of Bcr-Abl), characterized at the final stage by a loss of fat mass, muscle atrophy, anorexia and inflammation. The gut microbial 16S rDNA analysis, using PCR-Denaturating Gradient Gel Electrophoresis and quantitative PCR, reveals a dysbiosis and a selective modulation of Lactobacillus spp. (decrease of L. reuteri and L. johnsonii/gasseri in favor of L. murinus/animalis) in the BaF3 mice compared to the controls. The restoration of Lactobacillus species by oral supplementation with L. reuteri 100-23 and L. gasseri 311476 reduced the expression of atrophy markers (Atrogin-1, MuRF1, LC3, Cathepsin L) in the gastrocnemius and in the tibialis, a phenomenon correlated with a decrease of inflammatory cytokines (interleukin-6, monocyte chemoattractant protein-1, interleukin-4, granulocyte colony-stimulating factor, quantified by multiplex immuno-assay). These positive effects are strain- and/or species-specific since L. acidophilus NCFM supplementation does not impact on muscle atrophy markers and systemic inflammation. Altogether, these results suggest that the gut microbiota could constitute a novel therapeutic target in the management of leukemia-associated inflammation and related disorders in the muscle. |
| first_indexed | 2024-12-23T23:47:37Z |
| format | Article |
| id | doaj.art-a5d8875e57c147b28352e528878c764e |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-23T23:47:37Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-a5d8875e57c147b28352e528878c764e2022-12-21T17:25:29ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0176e3797110.1371/journal.pone.0037971Restoring specific lactobacilli levels decreases inflammation and muscle atrophy markers in an acute leukemia mouse model.Laure B BindelsRaphaël BeckOlivier SchakmanJennifer C MartinFabienne De BackerFlorence M SohetEvelyne M DewulfBarbara D PachikianAudrey M NeyrinckJean-Paul ThissenJulien VerraxPedro Buc CalderonBruno PotCorinne GrangettePatrice D CaniKaren P ScottNathalie M DelzenneThe gut microbiota has recently been proposed as a novel component in the regulation of host homeostasis and immunity. We have assessed for the first time the role of the gut microbiota in a mouse model of leukemia (transplantation of BaF3 cells containing ectopic expression of Bcr-Abl), characterized at the final stage by a loss of fat mass, muscle atrophy, anorexia and inflammation. The gut microbial 16S rDNA analysis, using PCR-Denaturating Gradient Gel Electrophoresis and quantitative PCR, reveals a dysbiosis and a selective modulation of Lactobacillus spp. (decrease of L. reuteri and L. johnsonii/gasseri in favor of L. murinus/animalis) in the BaF3 mice compared to the controls. The restoration of Lactobacillus species by oral supplementation with L. reuteri 100-23 and L. gasseri 311476 reduced the expression of atrophy markers (Atrogin-1, MuRF1, LC3, Cathepsin L) in the gastrocnemius and in the tibialis, a phenomenon correlated with a decrease of inflammatory cytokines (interleukin-6, monocyte chemoattractant protein-1, interleukin-4, granulocyte colony-stimulating factor, quantified by multiplex immuno-assay). These positive effects are strain- and/or species-specific since L. acidophilus NCFM supplementation does not impact on muscle atrophy markers and systemic inflammation. Altogether, these results suggest that the gut microbiota could constitute a novel therapeutic target in the management of leukemia-associated inflammation and related disorders in the muscle.http://europepmc.org/articles/PMC3384645?pdf=render |
| spellingShingle | Laure B Bindels Raphaël Beck Olivier Schakman Jennifer C Martin Fabienne De Backer Florence M Sohet Evelyne M Dewulf Barbara D Pachikian Audrey M Neyrinck Jean-Paul Thissen Julien Verrax Pedro Buc Calderon Bruno Pot Corinne Grangette Patrice D Cani Karen P Scott Nathalie M Delzenne Restoring specific lactobacilli levels decreases inflammation and muscle atrophy markers in an acute leukemia mouse model. PLoS ONE |
| title | Restoring specific lactobacilli levels decreases inflammation and muscle atrophy markers in an acute leukemia mouse model. |
| title_full | Restoring specific lactobacilli levels decreases inflammation and muscle atrophy markers in an acute leukemia mouse model. |
| title_fullStr | Restoring specific lactobacilli levels decreases inflammation and muscle atrophy markers in an acute leukemia mouse model. |
| title_full_unstemmed | Restoring specific lactobacilli levels decreases inflammation and muscle atrophy markers in an acute leukemia mouse model. |
| title_short | Restoring specific lactobacilli levels decreases inflammation and muscle atrophy markers in an acute leukemia mouse model. |
| title_sort | restoring specific lactobacilli levels decreases inflammation and muscle atrophy markers in an acute leukemia mouse model |
| url | http://europepmc.org/articles/PMC3384645?pdf=render |
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