Feed Insects as a Reservoir of Granadaene-Producing Lactococci
Insects are a component of the diet of different animal species and have been suggested as the major source of human dietary protein for the future. However, insects are also carriers of potentially pathogenic microbes that constitute a risk to food and feed safety. In this study, we reported the oc...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-05-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.848490/full |
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| author | Vera Neuzil-Bunesova Alejandro Ramirez Garcia Nikol Modrackova Marie Makovska Monika Sabolova Cathrin Spröer Boyke Bunk Jochen Blom Clarissa Schwab Clarissa Schwab Clarissa Schwab |
| author_facet | Vera Neuzil-Bunesova Alejandro Ramirez Garcia Nikol Modrackova Marie Makovska Monika Sabolova Cathrin Spröer Boyke Bunk Jochen Blom Clarissa Schwab Clarissa Schwab Clarissa Schwab |
| author_sort | Vera Neuzil-Bunesova |
| collection | DOAJ |
| description | Insects are a component of the diet of different animal species and have been suggested as the major source of human dietary protein for the future. However, insects are also carriers of potentially pathogenic microbes that constitute a risk to food and feed safety. In this study, we reported the occurrence of a hemolytic orange pigmented producing phenotype of Lactococcus garvieae/petauri/formosensis in the fecal microbiota of golden lion tamarins (Leontopithecus rosalia) and feed larvae (Zophobas atratus). Feed insects were identified as a regular source of L. garvieae/petauri/formosensis based on a reanalysis of available 16S rRNA gene libraries. Pan-genome analysis suggested the existence of four clusters within the L. garvieae/petauri/formosensis group. The presence of cyl cluster indicated that some strains of the L. garvieae/petauri/formosensis group produced a pigment similar to granadaene, an orange cytotoxic lipid produced by group B streptococci, including Streptococcus agalactiae. Pigment production by L. garvieae/petauri/formosensis strains was dependent on the presence of the fermentable sugars, with no pigment being observed at pH <4.7. The addition of buffering compounds or arginine, which can be metabolized to ammonium, restored pigment formation. In addition, pigment formation might be related to the source of peptone. These data suggest that edible insects are a possible source of granadaene-producing lactococci, which can be considered a pathogenic risk with zoonotic potential. |
| first_indexed | 2024-04-13T08:50:09Z |
| format | Article |
| id | doaj.art-a5daef8c78d449f48ea4e26311e9d2bf |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-13T08:50:09Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-a5daef8c78d449f48ea4e26311e9d2bf2022-12-22T02:53:30ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-05-011310.3389/fmicb.2022.848490848490Feed Insects as a Reservoir of Granadaene-Producing LactococciVera Neuzil-Bunesova0Alejandro Ramirez Garcia1Nikol Modrackova2Marie Makovska3Monika Sabolova4Cathrin Spröer5Boyke Bunk6Jochen Blom7Clarissa Schwab8Clarissa Schwab9Clarissa Schwab10Department of Microbiology, Nutrition and Dietetics, Czech University of Life Sciences Prague, Prague, CzechiaLaboratory of Food Biotechnology, Institute of Food, Nutrition and Health, ETH Zürich, Zurich, SwitzerlandDepartment of Microbiology, Nutrition and Dietetics, Czech University of Life Sciences Prague, Prague, CzechiaDepartment of Microbiology, Nutrition and Dietetics, Czech University of Life Sciences Prague, Prague, CzechiaDepartment of Microbiology, Nutrition and Dietetics, Czech University of Life Sciences Prague, Prague, CzechiaLeibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, GermanyLeibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, GermanyBioinformatics and Systems Biology, University Giessen, Giessen, GermanyDepartment of Microbiology, Nutrition and Dietetics, Czech University of Life Sciences Prague, Prague, CzechiaLaboratory of Food Biotechnology, Institute of Food, Nutrition and Health, ETH Zürich, Zurich, SwitzerlandDepartment of Biological and Chemical Engineering, Aarhus University, Aarhus, DenmarkInsects are a component of the diet of different animal species and have been suggested as the major source of human dietary protein for the future. However, insects are also carriers of potentially pathogenic microbes that constitute a risk to food and feed safety. In this study, we reported the occurrence of a hemolytic orange pigmented producing phenotype of Lactococcus garvieae/petauri/formosensis in the fecal microbiota of golden lion tamarins (Leontopithecus rosalia) and feed larvae (Zophobas atratus). Feed insects were identified as a regular source of L. garvieae/petauri/formosensis based on a reanalysis of available 16S rRNA gene libraries. Pan-genome analysis suggested the existence of four clusters within the L. garvieae/petauri/formosensis group. The presence of cyl cluster indicated that some strains of the L. garvieae/petauri/formosensis group produced a pigment similar to granadaene, an orange cytotoxic lipid produced by group B streptococci, including Streptococcus agalactiae. Pigment production by L. garvieae/petauri/formosensis strains was dependent on the presence of the fermentable sugars, with no pigment being observed at pH <4.7. The addition of buffering compounds or arginine, which can be metabolized to ammonium, restored pigment formation. In addition, pigment formation might be related to the source of peptone. These data suggest that edible insects are a possible source of granadaene-producing lactococci, which can be considered a pathogenic risk with zoonotic potential.https://www.frontiersin.org/articles/10.3389/fmicb.2022.848490/fullorange pigmentLactococcus garvieaeLactococcus petauriLactococcus formosensisinsectsmonkeys |
| spellingShingle | Vera Neuzil-Bunesova Alejandro Ramirez Garcia Nikol Modrackova Marie Makovska Monika Sabolova Cathrin Spröer Boyke Bunk Jochen Blom Clarissa Schwab Clarissa Schwab Clarissa Schwab Feed Insects as a Reservoir of Granadaene-Producing Lactococci Frontiers in Microbiology orange pigment Lactococcus garvieae Lactococcus petauri Lactococcus formosensis insects monkeys |
| title | Feed Insects as a Reservoir of Granadaene-Producing Lactococci |
| title_full | Feed Insects as a Reservoir of Granadaene-Producing Lactococci |
| title_fullStr | Feed Insects as a Reservoir of Granadaene-Producing Lactococci |
| title_full_unstemmed | Feed Insects as a Reservoir of Granadaene-Producing Lactococci |
| title_short | Feed Insects as a Reservoir of Granadaene-Producing Lactococci |
| title_sort | feed insects as a reservoir of granadaene producing lactococci |
| topic | orange pigment Lactococcus garvieae Lactococcus petauri Lactococcus formosensis insects monkeys |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.848490/full |
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