Proteomic response to phosphorus deficiency and aluminum stress of three aluminum-tolerant phosphobacteria isolated from acidic soils
Summary: Aluminum (Al)-tolerant phosphobacteria enhance plant growth in acidic soils by improving Al complexing and phosphorus (P) availability. However, the impact of Al stress and P deficiency on bacterial biochemistry and physiology remains unclear. We investigated the single and mutual effects o...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-10-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223019879 |
| _version_ | 1797647838138597376 |
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| author | Patricio Javier Barra Paola Duran Mabel Delgado Sharon Viscardi Stéphane Claverol Giovanni Larama Marc Dumont María de la Luz Mora |
| author_facet | Patricio Javier Barra Paola Duran Mabel Delgado Sharon Viscardi Stéphane Claverol Giovanni Larama Marc Dumont María de la Luz Mora |
| author_sort | Patricio Javier Barra |
| collection | DOAJ |
| description | Summary: Aluminum (Al)-tolerant phosphobacteria enhance plant growth in acidic soils by improving Al complexing and phosphorus (P) availability. However, the impact of Al stress and P deficiency on bacterial biochemistry and physiology remains unclear. We investigated the single and mutual effects of Al stress (10 mM) and P deficiency (0.05 mM) on the proteome of three aluminum-tolerant phosphobacteria: Enterobacter sp. 198, Enterobacter sp. RJAL6, and Klebsiella sp. RCJ4. Cultivated under varying conditions, P deficiency upregulated P metabolism proteins while Al exposure downregulated iron-sulfur and heme-containing proteins and upregulated iron acquisition proteins. This demonstrated that Al influence on iron homeostasis and bacterial central metabolism. This study offers crucial insights into bacterial behavior in acidic soils, benefiting the development of bioinoculants for crops facing Al toxicity and P deficiency. This investigation marks the first proteomic study on the interaction between high Al and P deficiency in acid soils-adapted bacteria. |
| first_indexed | 2024-03-11T15:23:23Z |
| format | Article |
| id | doaj.art-a5db112cb3f444038cba084d5deb0634 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-03-11T15:23:23Z |
| publishDate | 2023-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-a5db112cb3f444038cba084d5deb06342023-10-28T05:08:56ZengElsevieriScience2589-00422023-10-012610107910Proteomic response to phosphorus deficiency and aluminum stress of three aluminum-tolerant phosphobacteria isolated from acidic soilsPatricio Javier Barra0Paola Duran1Mabel Delgado2Sharon Viscardi3Stéphane Claverol4Giovanni Larama5Marc Dumont6María de la Luz Mora7Center of Plant, Soil Interaction and Natural Resources Biotechnology, Scientific and Technological Bioresource Nucleus, Universidad de La Frontera, Temuco 4811230, Chile; Biocontrol Research Laboratory, Universidad de La Frontera, Temuco 4811230, ChileCenter of Plant, Soil Interaction and Natural Resources Biotechnology, Scientific and Technological Bioresource Nucleus, Universidad de La Frontera, Temuco 4811230, Chile; Biocontrol Research Laboratory, Universidad de La Frontera, Temuco 4811230, Chile; Facultad de Ciencias Agropecuarias y Medioambiente, Departamento de Producción Agropecuaria, Universidad de La Frontera, Temuco 4811230, Chile; Corresponding authorCenter of Plant, Soil Interaction and Natural Resources Biotechnology, Scientific and Technological Bioresource Nucleus, Universidad de La Frontera, Temuco 4811230, ChileEscuela de la Salud, Campus San Francisco, Universidad Católica de Temuco, Temuco 4811230, ChilePlateforme Protéome, Centre Génomique Fonctionnelle de Bordeaux, Université de Bordeaux, Bordeaux, FranceCenter of Plant, Soil Interaction and Natural Resources Biotechnology, Scientific and Technological Bioresource Nucleus, Universidad de La Frontera, Temuco 4811230, Chile; Biocontrol Research Laboratory, Universidad de La Frontera, Temuco 4811230, ChileSchool of Biological Sciences, University of Southampton, Southampton, UKCenter of Plant, Soil Interaction and Natural Resources Biotechnology, Scientific and Technological Bioresource Nucleus, Universidad de La Frontera, Temuco 4811230, Chile; Corresponding authorSummary: Aluminum (Al)-tolerant phosphobacteria enhance plant growth in acidic soils by improving Al complexing and phosphorus (P) availability. However, the impact of Al stress and P deficiency on bacterial biochemistry and physiology remains unclear. We investigated the single and mutual effects of Al stress (10 mM) and P deficiency (0.05 mM) on the proteome of three aluminum-tolerant phosphobacteria: Enterobacter sp. 198, Enterobacter sp. RJAL6, and Klebsiella sp. RCJ4. Cultivated under varying conditions, P deficiency upregulated P metabolism proteins while Al exposure downregulated iron-sulfur and heme-containing proteins and upregulated iron acquisition proteins. This demonstrated that Al influence on iron homeostasis and bacterial central metabolism. This study offers crucial insights into bacterial behavior in acidic soils, benefiting the development of bioinoculants for crops facing Al toxicity and P deficiency. This investigation marks the first proteomic study on the interaction between high Al and P deficiency in acid soils-adapted bacteria.http://www.sciencedirect.com/science/article/pii/S2589004223019879soil scienceMicrobiologyBacteriologyproteomics |
| spellingShingle | Patricio Javier Barra Paola Duran Mabel Delgado Sharon Viscardi Stéphane Claverol Giovanni Larama Marc Dumont María de la Luz Mora Proteomic response to phosphorus deficiency and aluminum stress of three aluminum-tolerant phosphobacteria isolated from acidic soils iScience soil science Microbiology Bacteriology proteomics |
| title | Proteomic response to phosphorus deficiency and aluminum stress of three aluminum-tolerant phosphobacteria isolated from acidic soils |
| title_full | Proteomic response to phosphorus deficiency and aluminum stress of three aluminum-tolerant phosphobacteria isolated from acidic soils |
| title_fullStr | Proteomic response to phosphorus deficiency and aluminum stress of three aluminum-tolerant phosphobacteria isolated from acidic soils |
| title_full_unstemmed | Proteomic response to phosphorus deficiency and aluminum stress of three aluminum-tolerant phosphobacteria isolated from acidic soils |
| title_short | Proteomic response to phosphorus deficiency and aluminum stress of three aluminum-tolerant phosphobacteria isolated from acidic soils |
| title_sort | proteomic response to phosphorus deficiency and aluminum stress of three aluminum tolerant phosphobacteria isolated from acidic soils |
| topic | soil science Microbiology Bacteriology proteomics |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223019879 |
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