Single-Dose Vaccination of Recombinant Chimeric Newcastle Disease Virus (NDV) LaSota Vaccine Strain Expressing Infectious Bursal Disease Virus (IBDV) VP2 Gene Provides Full Protection against Genotype VII NDV and IBDV Challenge
Newcastle disease virus (NDV) and infectious bursal disease virus (IBDV) are the two most important and widespread viruses causing huge economic losses in the global poultry industry. Outbreaks of genotype VII NDV and IBDV variants in vaccinated poultry flocks call for genetically matched vaccines....
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-12-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/9/12/1483 |
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| author | Qilong Qiao Mingzhen Song Congcong Song Yihang Zhang Xiangdong Wang Qing Huang Baiyu Wang Panpan Yang Shiyi Zhao Yongtao Li Zeng Wang Jun Zhao |
| author_facet | Qilong Qiao Mingzhen Song Congcong Song Yihang Zhang Xiangdong Wang Qing Huang Baiyu Wang Panpan Yang Shiyi Zhao Yongtao Li Zeng Wang Jun Zhao |
| author_sort | Qilong Qiao |
| collection | DOAJ |
| description | Newcastle disease virus (NDV) and infectious bursal disease virus (IBDV) are the two most important and widespread viruses causing huge economic losses in the global poultry industry. Outbreaks of genotype VII NDV and IBDV variants in vaccinated poultry flocks call for genetically matched vaccines. In the present study, a genetic matched chimeric NDV LaSota vaccine strain expressing VP2 gene of IBDV variant, rLaS-VIIF/HN-VP2 was generated for the first time, in which both the F and HN genes of LaSota were replaced with those of the genotype VII NDV strain FJSW. The cleavage site of the FJSW strain F protein in the rLaS-VIIF/HN-VP2 genome was mutated to the avirulent motif found in LaSota. Expression of IBDV VP2 protein was confirmed by western blot. The rLaS-VIIF/HN-VP2 maintained the efficient replication ability in embryonated eggs, low pathogenicity and genetic stability comparable to that of parental LaSota virus. One dose oculonasal vaccination of one-week-old SPF chickens with rLaS-VIIF/HN-VP2 induced full protection against genotype VII NDV and IBDV lethal challenge. These results indicate that the rLaS-VIIF/HN-VP2 is a promising bivalent vaccine to prevent infections of IBDV and genotype VII NDV. |
| first_indexed | 2024-03-10T03:56:21Z |
| format | Article |
| id | doaj.art-a5debeeb86cb4fb5a75a5de649667c53 |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-10T03:56:21Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-a5debeeb86cb4fb5a75a5de649667c532023-11-23T10:54:58ZengMDPI AGVaccines2076-393X2021-12-01912148310.3390/vaccines9121483Single-Dose Vaccination of Recombinant Chimeric Newcastle Disease Virus (NDV) LaSota Vaccine Strain Expressing Infectious Bursal Disease Virus (IBDV) VP2 Gene Provides Full Protection against Genotype VII NDV and IBDV ChallengeQilong Qiao0Mingzhen Song1Congcong Song2Yihang Zhang3Xiangdong Wang4Qing Huang5Baiyu Wang6Panpan Yang7Shiyi Zhao8Yongtao Li9Zeng Wang10Jun Zhao11College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, ChinaNewcastle disease virus (NDV) and infectious bursal disease virus (IBDV) are the two most important and widespread viruses causing huge economic losses in the global poultry industry. Outbreaks of genotype VII NDV and IBDV variants in vaccinated poultry flocks call for genetically matched vaccines. In the present study, a genetic matched chimeric NDV LaSota vaccine strain expressing VP2 gene of IBDV variant, rLaS-VIIF/HN-VP2 was generated for the first time, in which both the F and HN genes of LaSota were replaced with those of the genotype VII NDV strain FJSW. The cleavage site of the FJSW strain F protein in the rLaS-VIIF/HN-VP2 genome was mutated to the avirulent motif found in LaSota. Expression of IBDV VP2 protein was confirmed by western blot. The rLaS-VIIF/HN-VP2 maintained the efficient replication ability in embryonated eggs, low pathogenicity and genetic stability comparable to that of parental LaSota virus. One dose oculonasal vaccination of one-week-old SPF chickens with rLaS-VIIF/HN-VP2 induced full protection against genotype VII NDV and IBDV lethal challenge. These results indicate that the rLaS-VIIF/HN-VP2 is a promising bivalent vaccine to prevent infections of IBDV and genotype VII NDV.https://www.mdpi.com/2076-393X/9/12/1483genotype VII Newcastle disease virusinfectious bursal disease virusVP2 genechimeric LaSota vaccine strain |
| spellingShingle | Qilong Qiao Mingzhen Song Congcong Song Yihang Zhang Xiangdong Wang Qing Huang Baiyu Wang Panpan Yang Shiyi Zhao Yongtao Li Zeng Wang Jun Zhao Single-Dose Vaccination of Recombinant Chimeric Newcastle Disease Virus (NDV) LaSota Vaccine Strain Expressing Infectious Bursal Disease Virus (IBDV) VP2 Gene Provides Full Protection against Genotype VII NDV and IBDV Challenge Vaccines genotype VII Newcastle disease virus infectious bursal disease virus VP2 gene chimeric LaSota vaccine strain |
| title | Single-Dose Vaccination of Recombinant Chimeric Newcastle Disease Virus (NDV) LaSota Vaccine Strain Expressing Infectious Bursal Disease Virus (IBDV) VP2 Gene Provides Full Protection against Genotype VII NDV and IBDV Challenge |
| title_full | Single-Dose Vaccination of Recombinant Chimeric Newcastle Disease Virus (NDV) LaSota Vaccine Strain Expressing Infectious Bursal Disease Virus (IBDV) VP2 Gene Provides Full Protection against Genotype VII NDV and IBDV Challenge |
| title_fullStr | Single-Dose Vaccination of Recombinant Chimeric Newcastle Disease Virus (NDV) LaSota Vaccine Strain Expressing Infectious Bursal Disease Virus (IBDV) VP2 Gene Provides Full Protection against Genotype VII NDV and IBDV Challenge |
| title_full_unstemmed | Single-Dose Vaccination of Recombinant Chimeric Newcastle Disease Virus (NDV) LaSota Vaccine Strain Expressing Infectious Bursal Disease Virus (IBDV) VP2 Gene Provides Full Protection against Genotype VII NDV and IBDV Challenge |
| title_short | Single-Dose Vaccination of Recombinant Chimeric Newcastle Disease Virus (NDV) LaSota Vaccine Strain Expressing Infectious Bursal Disease Virus (IBDV) VP2 Gene Provides Full Protection against Genotype VII NDV and IBDV Challenge |
| title_sort | single dose vaccination of recombinant chimeric newcastle disease virus ndv lasota vaccine strain expressing infectious bursal disease virus ibdv vp2 gene provides full protection against genotype vii ndv and ibdv challenge |
| topic | genotype VII Newcastle disease virus infectious bursal disease virus VP2 gene chimeric LaSota vaccine strain |
| url | https://www.mdpi.com/2076-393X/9/12/1483 |
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