The Recombinant Maize Ribosome-Inactivating Protein Transiently Reduces Viral Load in SHIV89.6 Infected Chinese Rhesus Macaques
Ribosome inactivating proteins (RIPs) inhibit protein synthesis by depurinating the large ribosomal RNA and some are found to possess anti-human immunodeficiency virus (HIV) activity. Maize ribosome inactivating protein (RIP) has an internal inactivation loop which is proteolytically removed for ful...
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| Format: | Article |
| Language: | English |
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MDPI AG
2015-01-01
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| Series: | Toxins |
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| Online Access: | http://www.mdpi.com/2072-6651/7/1/156 |
| _version_ | 1798002441598271488 |
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| author | Rui-Rui Wang Ka-Yee Au Hong-Yi Zheng Liang-Min Gao Xuan Zhang Rong-Hua Luo Sue Ka-Yee Law Amanda Nga-Sze Mak Kam-Bo Wong Ming-Xu Zhang Wei Pang Gao-Hong Zhang Pang-Chui Shaw Yong-Tang Zheng |
| author_facet | Rui-Rui Wang Ka-Yee Au Hong-Yi Zheng Liang-Min Gao Xuan Zhang Rong-Hua Luo Sue Ka-Yee Law Amanda Nga-Sze Mak Kam-Bo Wong Ming-Xu Zhang Wei Pang Gao-Hong Zhang Pang-Chui Shaw Yong-Tang Zheng |
| author_sort | Rui-Rui Wang |
| collection | DOAJ |
| description | Ribosome inactivating proteins (RIPs) inhibit protein synthesis by depurinating the large ribosomal RNA and some are found to possess anti-human immunodeficiency virus (HIV) activity. Maize ribosome inactivating protein (RIP) has an internal inactivation loop which is proteolytically removed for full catalytic activity. Here, we showed that the recombinant active maize RIP protected chimeric simian-human immunodeficiency virus (SHIV) 89.6-infected macaque peripheral blood mononuclear cells from lysis ex vivo and transiently reduced plasma viral load in SHIV89.6-infected rhesus macaque model. No evidence of immune dysregulation and other obvious side-effects was found in the treated macaques. Our work demonstrates the potential development of maize RIP as an anti-HIV agent without impeding systemic immune functions. |
| first_indexed | 2024-04-11T11:52:17Z |
| format | Article |
| id | doaj.art-a5e04ec8a6e347f28ef9d0959d5d7271 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-11T11:52:17Z |
| publishDate | 2015-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-a5e04ec8a6e347f28ef9d0959d5d72712022-12-22T04:25:17ZengMDPI AGToxins2072-66512015-01-017115616910.3390/toxins7010156toxins7010156The Recombinant Maize Ribosome-Inactivating Protein Transiently Reduces Viral Load in SHIV89.6 Infected Chinese Rhesus MacaquesRui-Rui Wang0Ka-Yee Au1Hong-Yi Zheng2Liang-Min Gao3Xuan Zhang4Rong-Hua Luo5Sue Ka-Yee Law6Amanda Nga-Sze Mak7Kam-Bo Wong8Ming-Xu Zhang9Wei Pang10Gao-Hong Zhang11Pang-Chui Shaw12Yong-Tang Zheng13Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, ChinaCenter for Protein Science and Crystallography, School of Life Sciences, the Chinese University of Hong Kong, Shatin N.T. 999077, Hong Kong, ChinaKey Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, ChinaDivision of HIV/AIDS and STI Control, Yuxi Centers for Disease Control and Prevention, Yuxi 653100, Yunnan, ChinaKey Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, ChinaKey Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, ChinaCenter for Protein Science and Crystallography, School of Life Sciences, the Chinese University of Hong Kong, Shatin N.T. 999077, Hong Kong, ChinaCenter for Protein Science and Crystallography, School of Life Sciences, the Chinese University of Hong Kong, Shatin N.T. 999077, Hong Kong, ChinaCenter for Protein Science and Crystallography, School of Life Sciences, the Chinese University of Hong Kong, Shatin N.T. 999077, Hong Kong, ChinaKey Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, ChinaKey Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, ChinaKey Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, ChinaCenter for Protein Science and Crystallography, School of Life Sciences, the Chinese University of Hong Kong, Shatin N.T. 999077, Hong Kong, ChinaKey Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, ChinaRibosome inactivating proteins (RIPs) inhibit protein synthesis by depurinating the large ribosomal RNA and some are found to possess anti-human immunodeficiency virus (HIV) activity. Maize ribosome inactivating protein (RIP) has an internal inactivation loop which is proteolytically removed for full catalytic activity. Here, we showed that the recombinant active maize RIP protected chimeric simian-human immunodeficiency virus (SHIV) 89.6-infected macaque peripheral blood mononuclear cells from lysis ex vivo and transiently reduced plasma viral load in SHIV89.6-infected rhesus macaque model. No evidence of immune dysregulation and other obvious side-effects was found in the treated macaques. Our work demonstrates the potential development of maize RIP as an anti-HIV agent without impeding systemic immune functions.http://www.mdpi.com/2072-6651/7/1/156maize RIPanti-HIVanimal modelviral load |
| spellingShingle | Rui-Rui Wang Ka-Yee Au Hong-Yi Zheng Liang-Min Gao Xuan Zhang Rong-Hua Luo Sue Ka-Yee Law Amanda Nga-Sze Mak Kam-Bo Wong Ming-Xu Zhang Wei Pang Gao-Hong Zhang Pang-Chui Shaw Yong-Tang Zheng The Recombinant Maize Ribosome-Inactivating Protein Transiently Reduces Viral Load in SHIV89.6 Infected Chinese Rhesus Macaques Toxins maize RIP anti-HIV animal model viral load |
| title | The Recombinant Maize Ribosome-Inactivating Protein Transiently Reduces Viral Load in SHIV89.6 Infected Chinese Rhesus Macaques |
| title_full | The Recombinant Maize Ribosome-Inactivating Protein Transiently Reduces Viral Load in SHIV89.6 Infected Chinese Rhesus Macaques |
| title_fullStr | The Recombinant Maize Ribosome-Inactivating Protein Transiently Reduces Viral Load in SHIV89.6 Infected Chinese Rhesus Macaques |
| title_full_unstemmed | The Recombinant Maize Ribosome-Inactivating Protein Transiently Reduces Viral Load in SHIV89.6 Infected Chinese Rhesus Macaques |
| title_short | The Recombinant Maize Ribosome-Inactivating Protein Transiently Reduces Viral Load in SHIV89.6 Infected Chinese Rhesus Macaques |
| title_sort | recombinant maize ribosome inactivating protein transiently reduces viral load in shiv89 6 infected chinese rhesus macaques |
| topic | maize RIP anti-HIV animal model viral load |
| url | http://www.mdpi.com/2072-6651/7/1/156 |
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