Influence of Caudovirales Phages on Humoral Immunity in Mice
Bacteriophages are promising antibacterial agents. Although they have been recognized as bacterial viruses and are considered to be non-interacting with eukaryotic cells, there is growing evidence that phages may have a significant impact on the immune system via interactions with macrophages, neutr...
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| Format: | Article |
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MDPI AG
2021-06-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/13/7/1241 |
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| author | Anton Chechushkov Yuliya Kozlova Ivan Baykov Vera Morozova Bogdana Kravchuk Tatyana Ushakova Alevtina Bardasheva Ekaterina Zelentsova Lina Al Allaf Artem Tikunov Valentin Vlassov Nina Tikunova |
| author_facet | Anton Chechushkov Yuliya Kozlova Ivan Baykov Vera Morozova Bogdana Kravchuk Tatyana Ushakova Alevtina Bardasheva Ekaterina Zelentsova Lina Al Allaf Artem Tikunov Valentin Vlassov Nina Tikunova |
| author_sort | Anton Chechushkov |
| collection | DOAJ |
| description | Bacteriophages are promising antibacterial agents. Although they have been recognized as bacterial viruses and are considered to be non-interacting with eukaryotic cells, there is growing evidence that phages may have a significant impact on the immune system via interactions with macrophages, neutrophils, and T-cell polarization. In this study, the influence of phages of podovirus, siphovirus, and myovirus morphotypes on humoral immunity of CD-1 mice was investigated. In addition, tissue distribution of the phages was tested in these mice. No common patterns were found either in the distribution of phages in mice or in changes in the levels of cytokines in the sera of mice once injected with phages. Importantly, pre-existing IgM-class antibodies directed against capsid proteins of phages with myovirus and siphovirus morphotypes were identified in mice before immunization. After triple immunization of CD1-mice with phages without any adjuvant, levels of anti-phage serum polyclonal IgG antibodies increased. Immunogenic phage proteins recognized by IgM and/or IgG antibodies were identified using Western blot analysis and mass spectrometry. In addition, mice serum collected after immunization demonstrated neutralizing properties, leading to a substantial decrease in infectivity of investigated phages with myovirus and siphovirus morphotypes. Moreover, serum samples collected before administration of these phages exhibited some ability to reduce the phage infectivity. Furthermore, Proteus phage PM16 with podovirus morphotype did not elicit IgM or IgG antibodies in immunized mice, and no neutralizing activities against PM16 were revealed in mouse serum samples before and after immunization. |
| first_indexed | 2024-03-10T10:01:50Z |
| format | Article |
| id | doaj.art-a5e1cb1a864c4eb7afb7367c468f2718 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T10:01:50Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-a5e1cb1a864c4eb7afb7367c468f27182023-11-22T01:52:59ZengMDPI AGViruses1999-49152021-06-01137124110.3390/v13071241Influence of Caudovirales Phages on Humoral Immunity in MiceAnton Chechushkov0Yuliya Kozlova1Ivan Baykov2Vera Morozova3Bogdana Kravchuk4Tatyana Ushakova5Alevtina Bardasheva6Ekaterina Zelentsova7Lina Al Allaf8Artem Tikunov9Valentin Vlassov10Nina Tikunova11Laboratory of Molecular Microbiology, Institute of Chemical Biology and Fundamental Medicine Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, RussiaLaboratory of Molecular Microbiology, Institute of Chemical Biology and Fundamental Medicine Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, RussiaLaboratory of Molecular Microbiology, Institute of Chemical Biology and Fundamental Medicine Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, RussiaLaboratory of Molecular Microbiology, Institute of Chemical Biology and Fundamental Medicine Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, RussiaLaboratory of Molecular Microbiology, Institute of Chemical Biology and Fundamental Medicine Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, RussiaLaboratory of Molecular Microbiology, Institute of Chemical Biology and Fundamental Medicine Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, RussiaLaboratory of Molecular Microbiology, Institute of Chemical Biology and Fundamental Medicine Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, RussiaInternational Tomography Center Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, RussiaLaboratory of Molecular Microbiology, Institute of Chemical Biology and Fundamental Medicine Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, RussiaLaboratory of Molecular Microbiology, Institute of Chemical Biology and Fundamental Medicine Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, RussiaLaboratory of Molecular Microbiology, Institute of Chemical Biology and Fundamental Medicine Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, RussiaLaboratory of Molecular Microbiology, Institute of Chemical Biology and Fundamental Medicine Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, RussiaBacteriophages are promising antibacterial agents. Although they have been recognized as bacterial viruses and are considered to be non-interacting with eukaryotic cells, there is growing evidence that phages may have a significant impact on the immune system via interactions with macrophages, neutrophils, and T-cell polarization. In this study, the influence of phages of podovirus, siphovirus, and myovirus morphotypes on humoral immunity of CD-1 mice was investigated. In addition, tissue distribution of the phages was tested in these mice. No common patterns were found either in the distribution of phages in mice or in changes in the levels of cytokines in the sera of mice once injected with phages. Importantly, pre-existing IgM-class antibodies directed against capsid proteins of phages with myovirus and siphovirus morphotypes were identified in mice before immunization. After triple immunization of CD1-mice with phages without any adjuvant, levels of anti-phage serum polyclonal IgG antibodies increased. Immunogenic phage proteins recognized by IgM and/or IgG antibodies were identified using Western blot analysis and mass spectrometry. In addition, mice serum collected after immunization demonstrated neutralizing properties, leading to a substantial decrease in infectivity of investigated phages with myovirus and siphovirus morphotypes. Moreover, serum samples collected before administration of these phages exhibited some ability to reduce the phage infectivity. Furthermore, Proteus phage PM16 with podovirus morphotype did not elicit IgM or IgG antibodies in immunized mice, and no neutralizing activities against PM16 were revealed in mouse serum samples before and after immunization.https://www.mdpi.com/1999-4915/13/7/1241bacteriophageimmunogenicityantibodiesIgMIgGneutralization |
| spellingShingle | Anton Chechushkov Yuliya Kozlova Ivan Baykov Vera Morozova Bogdana Kravchuk Tatyana Ushakova Alevtina Bardasheva Ekaterina Zelentsova Lina Al Allaf Artem Tikunov Valentin Vlassov Nina Tikunova Influence of Caudovirales Phages on Humoral Immunity in Mice Viruses bacteriophage immunogenicity antibodies IgM IgG neutralization |
| title | Influence of Caudovirales Phages on Humoral Immunity in Mice |
| title_full | Influence of Caudovirales Phages on Humoral Immunity in Mice |
| title_fullStr | Influence of Caudovirales Phages on Humoral Immunity in Mice |
| title_full_unstemmed | Influence of Caudovirales Phages on Humoral Immunity in Mice |
| title_short | Influence of Caudovirales Phages on Humoral Immunity in Mice |
| title_sort | influence of caudovirales phages on humoral immunity in mice |
| topic | bacteriophage immunogenicity antibodies IgM IgG neutralization |
| url | https://www.mdpi.com/1999-4915/13/7/1241 |
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