A unique sigma/anti-sigma system in the actinomycete Actinoplanes missouriensis
Abstract Bacteria of the genus Actinoplanes form sporangia that contain dormant sporangiospores which, upon contact with water, release motile spores (zoospores) through a process called sporangium dehiscence. Here, we set out to study the molecular mechanisms behind sporangium dehiscence in Actinop...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-12-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-023-44291-y |
| _version_ | 1797376926600396800 |
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| author | Takeaki Tezuka Kyota Mitsuyama Risa Date Yasuo Ohnishi |
| author_facet | Takeaki Tezuka Kyota Mitsuyama Risa Date Yasuo Ohnishi |
| author_sort | Takeaki Tezuka |
| collection | DOAJ |
| description | Abstract Bacteria of the genus Actinoplanes form sporangia that contain dormant sporangiospores which, upon contact with water, release motile spores (zoospores) through a process called sporangium dehiscence. Here, we set out to study the molecular mechanisms behind sporangium dehiscence in Actinoplanes missouriensis and discover a sigma/anti-sigma system with unique features. Protein σSsdA contains a functional sigma factor domain and an anti-sigma factor antagonist domain, while protein SipA contains an anti-sigma factor domain and an anti-sigma factor antagonist domain. Remarkably, the two proteins interact with each other via the anti-sigma factor antagonist domain of σSsdA and the anti-sigma factor domain of SipA. Although it remains unclear whether the SipA/σSsdA system plays direct roles in sporangium dehiscence, the system seems to modulate oxidative stress responses in zoospores. In addition, we identify a two-component regulatory system (RsdK-RsdR) that represses initiation of sporangium dehiscence. |
| first_indexed | 2024-03-08T19:45:39Z |
| format | Article |
| id | doaj.art-a5e3b64738564375869d37a6e7cbee01 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-03-08T19:45:39Z |
| publishDate | 2023-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-a5e3b64738564375869d37a6e7cbee012023-12-24T12:22:51ZengNature PortfolioNature Communications2041-17232023-12-0114111610.1038/s41467-023-44291-yA unique sigma/anti-sigma system in the actinomycete Actinoplanes missouriensisTakeaki Tezuka0Kyota Mitsuyama1Risa Date2Yasuo Ohnishi3Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of TokyoDepartment of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of TokyoDepartment of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of TokyoDepartment of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of TokyoAbstract Bacteria of the genus Actinoplanes form sporangia that contain dormant sporangiospores which, upon contact with water, release motile spores (zoospores) through a process called sporangium dehiscence. Here, we set out to study the molecular mechanisms behind sporangium dehiscence in Actinoplanes missouriensis and discover a sigma/anti-sigma system with unique features. Protein σSsdA contains a functional sigma factor domain and an anti-sigma factor antagonist domain, while protein SipA contains an anti-sigma factor domain and an anti-sigma factor antagonist domain. Remarkably, the two proteins interact with each other via the anti-sigma factor antagonist domain of σSsdA and the anti-sigma factor domain of SipA. Although it remains unclear whether the SipA/σSsdA system plays direct roles in sporangium dehiscence, the system seems to modulate oxidative stress responses in zoospores. In addition, we identify a two-component regulatory system (RsdK-RsdR) that represses initiation of sporangium dehiscence.https://doi.org/10.1038/s41467-023-44291-y |
| spellingShingle | Takeaki Tezuka Kyota Mitsuyama Risa Date Yasuo Ohnishi A unique sigma/anti-sigma system in the actinomycete Actinoplanes missouriensis Nature Communications |
| title | A unique sigma/anti-sigma system in the actinomycete Actinoplanes missouriensis |
| title_full | A unique sigma/anti-sigma system in the actinomycete Actinoplanes missouriensis |
| title_fullStr | A unique sigma/anti-sigma system in the actinomycete Actinoplanes missouriensis |
| title_full_unstemmed | A unique sigma/anti-sigma system in the actinomycete Actinoplanes missouriensis |
| title_short | A unique sigma/anti-sigma system in the actinomycete Actinoplanes missouriensis |
| title_sort | unique sigma anti sigma system in the actinomycete actinoplanes missouriensis |
| url | https://doi.org/10.1038/s41467-023-44291-y |
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