Simplified Regeneration Protocol for <i>Cycas revoluta</i> Thunb. Mature Zygotic Embryos
Mature zygotic embryos of Cycas revoluta Thunb. were used as explants to investigate direct and indirect organogenesis. Explants were incubated on half-strength Murashige and Skoog (½ MS) basal medium supplemented with various plant growth regulators, singly or in combination (all at 0.5 mg l-1): 6-...
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| Format: | Article |
| Language: | English |
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Society of Land Measurements and Cadastre from Transylvania (SMTCT)
2015-03-01
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| Series: | Notulae Scientia Biologicae |
| Online Access: | http://www.notulaebiologicae.ro/index.php/nsb/article/view/9482 |
| _version_ | 1818500781020545024 |
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| author | Rohangiz NADERI Khadije MOHAISENI Jaime A. TEIXEIRA DA SILVA Mansour OMIDI Behjat NADERI |
| author_facet | Rohangiz NADERI Khadije MOHAISENI Jaime A. TEIXEIRA DA SILVA Mansour OMIDI Behjat NADERI |
| author_sort | Rohangiz NADERI |
| collection | DOAJ |
| description | Mature zygotic embryos of Cycas revoluta Thunb. were used as explants to investigate direct and indirect organogenesis. Explants were incubated on half-strength Murashige and Skoog (½ MS) basal medium supplemented with various plant growth regulators, singly or in combination (all at 0.5 mg l-1): 6-benzyladenine (BA), kinetin (Kin), 2,4-dichlorophenoxyacetic acid (2,4-D), Kin×2,4-D, BA×Kin and BA×2,4-D. Cultures were placed at a low light intensity (4 µmol m-2 s-1 PPFD). Adventitious shoot regeneration was observed in the presence of 0.5 mg l-1BA after 35 days. The highest number of direct and indirect shoots per zygotic embryo was 3.67 and 29.67, respectively. Roots were induced on indirect shoots by continuous culture on rooting medium (½ MS, 0.1 mg l-1 1-naphthaleneacetic acid) and hardened successfully in perlite. Each rooted plantlet with pinnate leaves and a primary tap root was individually isolated and acclimatized 185 days after the beginning of culture, with a 10% success rate. |
| first_indexed | 2024-12-10T20:47:25Z |
| format | Article |
| id | doaj.art-a5e509db10e84383acf32aed88f21aeb |
| institution | Directory Open Access Journal |
| issn | 2067-3205 2067-3264 |
| language | English |
| last_indexed | 2024-12-10T20:47:25Z |
| publishDate | 2015-03-01 |
| publisher | Society of Land Measurements and Cadastre from Transylvania (SMTCT) |
| record_format | Article |
| series | Notulae Scientia Biologicae |
| spelling | doaj.art-a5e509db10e84383acf32aed88f21aeb2022-12-22T01:34:11ZengSociety of Land Measurements and Cadastre from Transylvania (SMTCT)Notulae Scientia Biologicae2067-32052067-32642015-03-0171626510.15835/nsb7194828118Simplified Regeneration Protocol for <i>Cycas revoluta</i> Thunb. Mature Zygotic EmbryosRohangiz NADERI0Khadije MOHAISENI1Jaime A. TEIXEIRA DA SILVA2Mansour OMIDI3Behjat NADERI4University of Tehran, Faculty of Agricultural Science and Engineering, Department of Horticulture, 31587-77871, TehranUniversity of Tehran, Faculty of Agricultural Science and Engineering, Department of Horticulture, 31587-77871, TehranP. O. Box 7, Miki-cho post office, Ikenobe 3011-2, Kagawa-ken, 761-0799University of Tehran, Faculty of Agricultural Science and Engineering, Department of Agronomy and Plant Breeding, 31587-77871, TehranUniversity of Tehran, Faculty of Agricultural Science and Engineering, Department of Agronomy and Plant Breeding, 31587-77871, TehranMature zygotic embryos of Cycas revoluta Thunb. were used as explants to investigate direct and indirect organogenesis. Explants were incubated on half-strength Murashige and Skoog (½ MS) basal medium supplemented with various plant growth regulators, singly or in combination (all at 0.5 mg l-1): 6-benzyladenine (BA), kinetin (Kin), 2,4-dichlorophenoxyacetic acid (2,4-D), Kin×2,4-D, BA×Kin and BA×2,4-D. Cultures were placed at a low light intensity (4 µmol m-2 s-1 PPFD). Adventitious shoot regeneration was observed in the presence of 0.5 mg l-1BA after 35 days. The highest number of direct and indirect shoots per zygotic embryo was 3.67 and 29.67, respectively. Roots were induced on indirect shoots by continuous culture on rooting medium (½ MS, 0.1 mg l-1 1-naphthaleneacetic acid) and hardened successfully in perlite. Each rooted plantlet with pinnate leaves and a primary tap root was individually isolated and acclimatized 185 days after the beginning of culture, with a 10% success rate.http://www.notulaebiologicae.ro/index.php/nsb/article/view/9482 |
| spellingShingle | Rohangiz NADERI Khadije MOHAISENI Jaime A. TEIXEIRA DA SILVA Mansour OMIDI Behjat NADERI Simplified Regeneration Protocol for <i>Cycas revoluta</i> Thunb. Mature Zygotic Embryos Notulae Scientia Biologicae |
| title | Simplified Regeneration Protocol for <i>Cycas revoluta</i> Thunb. Mature Zygotic Embryos |
| title_full | Simplified Regeneration Protocol for <i>Cycas revoluta</i> Thunb. Mature Zygotic Embryos |
| title_fullStr | Simplified Regeneration Protocol for <i>Cycas revoluta</i> Thunb. Mature Zygotic Embryos |
| title_full_unstemmed | Simplified Regeneration Protocol for <i>Cycas revoluta</i> Thunb. Mature Zygotic Embryos |
| title_short | Simplified Regeneration Protocol for <i>Cycas revoluta</i> Thunb. Mature Zygotic Embryos |
| title_sort | simplified regeneration protocol for lt i gt cycas revoluta lt i gt thunb mature zygotic embryos |
| url | http://www.notulaebiologicae.ro/index.php/nsb/article/view/9482 |
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