Control of fatty acid distribution in phosphatidylcholine of spinach leaves
The acylation of lysophosphatidylcholine by enzyme preparations from spinach leaves was studied. The acylation reaction was followed by the incorporation of 14C-labeled fatty acids from the respective coenzyme A derivatives into phosphatidylcholine. The subcellular fraction with the highest specific...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
1971-07-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520394906 |
| _version_ | 1818385640986771456 |
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| author | K.A. DEVOR J.B. MUDD |
| author_facet | K.A. DEVOR J.B. MUDD |
| author_sort | K.A. DEVOR |
| collection | DOAJ |
| description | The acylation of lysophosphatidylcholine by enzyme preparations from spinach leaves was studied. The acylation reaction was followed by the incorporation of 14C-labeled fatty acids from the respective coenzyme A derivatives into phosphatidylcholine. The subcellular fraction with the highest specific activity was the microsomal fraction. Contaminating thioesterase activity which was encountered was inhibited by treatment with sodium dodecyl sulfate. The acyltransferase activity was only mildly inhibited by sulfhydryl reagents. Labeled fatty acid was primarily incorporated into phosphatidylcholine. When saturated and unsaturated fatty acyl CoA derivatives were used, the saturated derivatives were incorporated primarily into the 1-position of the glycerol moiety, and the unsaturated fatty acids went primarily to the 2-position. This pattern of incorporation agrees with the fatty acid distribution in vivo. |
| first_indexed | 2024-12-14T03:41:23Z |
| format | Article |
| id | doaj.art-a5e715aac6fd4cd29ee0c6911d895f1b |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-14T03:41:23Z |
| publishDate | 1971-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-a5e715aac6fd4cd29ee0c6911d895f1b2022-12-21T23:18:29ZengElsevierJournal of Lipid Research0022-22751971-07-01124412419Control of fatty acid distribution in phosphatidylcholine of spinach leavesK.A. DEVOR0J.B. MUDD1Department of Biochemistry, University of California, Riverside, California 92502Department of Biochemistry, University of California, Riverside, California 92502The acylation of lysophosphatidylcholine by enzyme preparations from spinach leaves was studied. The acylation reaction was followed by the incorporation of 14C-labeled fatty acids from the respective coenzyme A derivatives into phosphatidylcholine. The subcellular fraction with the highest specific activity was the microsomal fraction. Contaminating thioesterase activity which was encountered was inhibited by treatment with sodium dodecyl sulfate. The acyltransferase activity was only mildly inhibited by sulfhydryl reagents. Labeled fatty acid was primarily incorporated into phosphatidylcholine. When saturated and unsaturated fatty acyl CoA derivatives were used, the saturated derivatives were incorporated primarily into the 1-position of the glycerol moiety, and the unsaturated fatty acids went primarily to the 2-position. This pattern of incorporation agrees with the fatty acid distribution in vivo.http://www.sciencedirect.com/science/article/pii/S0022227520394906enzymic controlfatty acid distributionfatty acyl coenzyme A |
| spellingShingle | K.A. DEVOR J.B. MUDD Control of fatty acid distribution in phosphatidylcholine of spinach leaves Journal of Lipid Research enzymic control fatty acid distribution fatty acyl coenzyme A |
| title | Control of fatty acid distribution in phosphatidylcholine of spinach leaves |
| title_full | Control of fatty acid distribution in phosphatidylcholine of spinach leaves |
| title_fullStr | Control of fatty acid distribution in phosphatidylcholine of spinach leaves |
| title_full_unstemmed | Control of fatty acid distribution in phosphatidylcholine of spinach leaves |
| title_short | Control of fatty acid distribution in phosphatidylcholine of spinach leaves |
| title_sort | control of fatty acid distribution in phosphatidylcholine of spinach leaves |
| topic | enzymic control fatty acid distribution fatty acyl coenzyme A |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520394906 |
| work_keys_str_mv | AT kadevor controloffattyaciddistributioninphosphatidylcholineofspinachleaves AT jbmudd controloffattyaciddistributioninphosphatidylcholineofspinachleaves |