The spa Typing and Characterization of Nasal Carriage Methicillin-resistant Staphylococcus aureus Isolates From Healthy Children
Background: The Methicillin-resistant Staphylococcus aureus (MRSA) strains cause wide spectrum infections in hospitals and are responsible for the majority of the community-acquired infections. The emergence of community-acquired MRSA in patients without risk factors represents a high risk for publi...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Mazandaran University of Medical Sciences
2020-01-01
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| Series: | Journal of Pediatrics Review |
| Subjects: | |
| Online Access: | http://jpr.mazums.ac.ir/article-1-251-en.html |
| _version_ | 1828311827691339776 |
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| author | Shaghayegh Rezai Reza Valadan Fatemeh Ahangarkani Mohammad Sadegh Rezai |
| author_facet | Shaghayegh Rezai Reza Valadan Fatemeh Ahangarkani Mohammad Sadegh Rezai |
| author_sort | Shaghayegh Rezai |
| collection | DOAJ |
| description | Background: The Methicillin-resistant Staphylococcus aureus (MRSA) strains cause wide spectrum infections in hospitals and are responsible for the majority of the community-acquired infections. The emergence of community-acquired MRSA in patients without risk factors represents a high risk for public health.
Objectives: In this study, we aimed to molecular typing the nasal carriage MRSA strains harbored mecA gene isolated from healthy children living in the North of Iran and also to determine the pattern of vancomycin susceptibility in these isolates.
Methods: The MRSA strains were identified using standard microbiological and molecular procedures. The antibiotic susceptibility test was performed according to the clinical and laboratory standards institute. The MRSA strains were characterized by spa typing.
Results: In total, 14 different patterns of spa genes were obtained from MRSA isolates in this study, which included t790 (23.07%), t2962 (15.38%), t267 (15.38%), t159 (7.69%) and t701, t094, t1816, t11332, t937, t2135, t180, t160 [1 (3.84%) isolate per each type]. The rate of resistant isolates to cefoxitin, , cefalotin, clindamycin, cefazolin, co-amoxiclav, co-trimoxazole, and cephalexin antibiotics were 69.23%, 100%, 46.15%, 96.15%, 100%, 3.48%, and 100%, respectively. In total, six (23.07%) revealed vancomycin-intermediate resistant phenotype and five (19.23%) isolates revealed vancomycin-resistant S. aureus phenotype.
Conclusions: This study showed the different variants of MRSA strains based on molecular typing, among healthy children. Continuous surveillance studies to monitor MRSA should be encouraged to gain a better understanding of the circulating MRSA strains and prevent community spread of this pathogen. |
| first_indexed | 2024-04-13T16:05:58Z |
| format | Article |
| id | doaj.art-a5f1c6c7d5b24bed9b30964ae26b392c |
| institution | Directory Open Access Journal |
| issn | 2322-4398 2322-4401 |
| language | English |
| last_indexed | 2024-04-13T16:05:58Z |
| publishDate | 2020-01-01 |
| publisher | Mazandaran University of Medical Sciences |
| record_format | Article |
| series | Journal of Pediatrics Review |
| spelling | doaj.art-a5f1c6c7d5b24bed9b30964ae26b392c2022-12-22T02:40:23ZengMazandaran University of Medical SciencesJournal of Pediatrics Review2322-43982322-44012020-01-01815964The spa Typing and Characterization of Nasal Carriage Methicillin-resistant Staphylococcus aureus Isolates From Healthy ChildrenShaghayegh Rezai0Reza Valadan1Fatemeh Ahangarkani2Mohammad Sadegh Rezai3 Pediatric Infectious Diseases Research Center, Mazandaran University of Medical Sciences, Sari, Iran. Molecular and Cell Biology Research Center, Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran. Antimicrobial Resistance Research Center, Student Research Committee, Mazandaran University of Medical Sciences, Sari, Iran. Pediatric Infectious Diseases Research Center, Mazandaran University of Medical Sciences, Sari, Iran. Background: The Methicillin-resistant Staphylococcus aureus (MRSA) strains cause wide spectrum infections in hospitals and are responsible for the majority of the community-acquired infections. The emergence of community-acquired MRSA in patients without risk factors represents a high risk for public health. Objectives: In this study, we aimed to molecular typing the nasal carriage MRSA strains harbored mecA gene isolated from healthy children living in the North of Iran and also to determine the pattern of vancomycin susceptibility in these isolates. Methods: The MRSA strains were identified using standard microbiological and molecular procedures. The antibiotic susceptibility test was performed according to the clinical and laboratory standards institute. The MRSA strains were characterized by spa typing. Results: In total, 14 different patterns of spa genes were obtained from MRSA isolates in this study, which included t790 (23.07%), t2962 (15.38%), t267 (15.38%), t159 (7.69%) and t701, t094, t1816, t11332, t937, t2135, t180, t160 [1 (3.84%) isolate per each type]. The rate of resistant isolates to cefoxitin, , cefalotin, clindamycin, cefazolin, co-amoxiclav, co-trimoxazole, and cephalexin antibiotics were 69.23%, 100%, 46.15%, 96.15%, 100%, 3.48%, and 100%, respectively. In total, six (23.07%) revealed vancomycin-intermediate resistant phenotype and five (19.23%) isolates revealed vancomycin-resistant S. aureus phenotype. Conclusions: This study showed the different variants of MRSA strains based on molecular typing, among healthy children. Continuous surveillance studies to monitor MRSA should be encouraged to gain a better understanding of the circulating MRSA strains and prevent community spread of this pathogen.http://jpr.mazums.ac.ir/article-1-251-en.htmlmethicillin-resistant staphylococcus aureusmolecular typingchildrencommunity-acquired infections |
| spellingShingle | Shaghayegh Rezai Reza Valadan Fatemeh Ahangarkani Mohammad Sadegh Rezai The spa Typing and Characterization of Nasal Carriage Methicillin-resistant Staphylococcus aureus Isolates From Healthy Children Journal of Pediatrics Review methicillin-resistant staphylococcus aureus molecular typing children community-acquired infections |
| title | The spa Typing and Characterization of Nasal Carriage Methicillin-resistant Staphylococcus aureus Isolates From Healthy Children |
| title_full | The spa Typing and Characterization of Nasal Carriage Methicillin-resistant Staphylococcus aureus Isolates From Healthy Children |
| title_fullStr | The spa Typing and Characterization of Nasal Carriage Methicillin-resistant Staphylococcus aureus Isolates From Healthy Children |
| title_full_unstemmed | The spa Typing and Characterization of Nasal Carriage Methicillin-resistant Staphylococcus aureus Isolates From Healthy Children |
| title_short | The spa Typing and Characterization of Nasal Carriage Methicillin-resistant Staphylococcus aureus Isolates From Healthy Children |
| title_sort | spa typing and characterization of nasal carriage methicillin resistant staphylococcus aureus isolates from healthy children |
| topic | methicillin-resistant staphylococcus aureus molecular typing children community-acquired infections |
| url | http://jpr.mazums.ac.ir/article-1-251-en.html |
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