Genome-Wide Analysis of Ribosomal Protein GhRPS6 and Its Role in Cotton Verticillium Wilt Resistance
Verticillium wilt is threatening the world’s cotton production. The pathogenic fungus <i>Verticillium dahliae</i> can survive in the soil in the form of microsclerotia for a long time, colonize through the root of cotton, and invade into vascular bundles, causing yellowing and wilting of...
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MDPI AG
2021-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/4/1795 |
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| author | Dandan Zhu Xiangyue Zhang Jinglong Zhou Yajie Wu Xiaojian Zhang Zili Feng Feng Wei Lihong Zhao Yalin Zhang Yongqiang Shi Hongjie Feng Heqin Zhu |
| author_facet | Dandan Zhu Xiangyue Zhang Jinglong Zhou Yajie Wu Xiaojian Zhang Zili Feng Feng Wei Lihong Zhao Yalin Zhang Yongqiang Shi Hongjie Feng Heqin Zhu |
| author_sort | Dandan Zhu |
| collection | DOAJ |
| description | Verticillium wilt is threatening the world’s cotton production. The pathogenic fungus <i>Verticillium dahliae</i> can survive in the soil in the form of microsclerotia for a long time, colonize through the root of cotton, and invade into vascular bundles, causing yellowing and wilting of cotton leaves, and in serious cases, leading to plant death. Breeding resistant varieties is the most economical and effective method to control Verticillium wilt. In previous studies, proteomic analysis was carried out on different cotton varieties inoculated with <i>V. dahliae</i> strain Vd080. It was found that GhRPS6 was phosphorylated after inoculation, and the phosphorylation level in resistant cultivars was 1.5 times than that in susceptible cultivars. In this study, knockdown of <i>GhRPS6</i> expression results in the reduction of SA and JA content, and suppresses a series of defensive response, enhancing cotton plants susceptibility to <i>V. dahliae</i>. Overexpression in Arabidopsis <i>thaliana</i> transgenic plants was found to be more resistant to <i>V. dahliae</i>. Further, serines at 237 and 240 were mutated to phenylalanine, respectively and jointly. The transgenic <i>Arabidopsis</i> plants demonstrated that seri-237 compromised the plant resistance to <i>V. dahliae</i>. Subcellular localization in <i>Nicotiana benthamiana</i> showed that GhRPS6 was localized in the nucleus. Additionally, the pathogen inoculation and phosphorylation site mutation did not change its localization. These results indicate that GhRPS6 is a potential molecular target for improving resistance to Verticillium wilt in cotton. This lays a foundation for breeding disease-resistant varieties. |
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| language | English |
| last_indexed | 2024-03-09T00:57:53Z |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-a5f28f6256784cdba3465754c6679c1b2023-12-11T16:46:10ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-02-01224179510.3390/ijms22041795Genome-Wide Analysis of Ribosomal Protein GhRPS6 and Its Role in Cotton Verticillium Wilt ResistanceDandan Zhu0Xiangyue Zhang1Jinglong Zhou2Yajie Wu3Xiaojian Zhang4Zili Feng5Feng Wei6Lihong Zhao7Yalin Zhang8Yongqiang Shi9Hongjie Feng10Heqin Zhu11Zhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Life Science, Zhengzhou University, Zhengzhou 450001, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaSchool of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, ChinaSchool of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaVerticillium wilt is threatening the world’s cotton production. The pathogenic fungus <i>Verticillium dahliae</i> can survive in the soil in the form of microsclerotia for a long time, colonize through the root of cotton, and invade into vascular bundles, causing yellowing and wilting of cotton leaves, and in serious cases, leading to plant death. Breeding resistant varieties is the most economical and effective method to control Verticillium wilt. In previous studies, proteomic analysis was carried out on different cotton varieties inoculated with <i>V. dahliae</i> strain Vd080. It was found that GhRPS6 was phosphorylated after inoculation, and the phosphorylation level in resistant cultivars was 1.5 times than that in susceptible cultivars. In this study, knockdown of <i>GhRPS6</i> expression results in the reduction of SA and JA content, and suppresses a series of defensive response, enhancing cotton plants susceptibility to <i>V. dahliae</i>. Overexpression in Arabidopsis <i>thaliana</i> transgenic plants was found to be more resistant to <i>V. dahliae</i>. Further, serines at 237 and 240 were mutated to phenylalanine, respectively and jointly. The transgenic <i>Arabidopsis</i> plants demonstrated that seri-237 compromised the plant resistance to <i>V. dahliae</i>. Subcellular localization in <i>Nicotiana benthamiana</i> showed that GhRPS6 was localized in the nucleus. Additionally, the pathogen inoculation and phosphorylation site mutation did not change its localization. These results indicate that GhRPS6 is a potential molecular target for improving resistance to Verticillium wilt in cotton. This lays a foundation for breeding disease-resistant varieties.https://www.mdpi.com/1422-0067/22/4/1795cottonVerticillium wiltribosomal proteinphosphorylation |
| spellingShingle | Dandan Zhu Xiangyue Zhang Jinglong Zhou Yajie Wu Xiaojian Zhang Zili Feng Feng Wei Lihong Zhao Yalin Zhang Yongqiang Shi Hongjie Feng Heqin Zhu Genome-Wide Analysis of Ribosomal Protein GhRPS6 and Its Role in Cotton Verticillium Wilt Resistance International Journal of Molecular Sciences cotton Verticillium wilt ribosomal protein phosphorylation |
| title | Genome-Wide Analysis of Ribosomal Protein GhRPS6 and Its Role in Cotton Verticillium Wilt Resistance |
| title_full | Genome-Wide Analysis of Ribosomal Protein GhRPS6 and Its Role in Cotton Verticillium Wilt Resistance |
| title_fullStr | Genome-Wide Analysis of Ribosomal Protein GhRPS6 and Its Role in Cotton Verticillium Wilt Resistance |
| title_full_unstemmed | Genome-Wide Analysis of Ribosomal Protein GhRPS6 and Its Role in Cotton Verticillium Wilt Resistance |
| title_short | Genome-Wide Analysis of Ribosomal Protein GhRPS6 and Its Role in Cotton Verticillium Wilt Resistance |
| title_sort | genome wide analysis of ribosomal protein ghrps6 and its role in cotton verticillium wilt resistance |
| topic | cotton Verticillium wilt ribosomal protein phosphorylation |
| url | https://www.mdpi.com/1422-0067/22/4/1795 |
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