Cytological and chromosomal damages induced by tartrazine and two classes (III and IV) of caramel food dyes
Food colors such as tartrazine (E102), ammonia caramel (E150c), sulphite ammonia caramel (E150d) are widely used in the food sector. These additives are thought to be a long-term toxicity source. The goal of our research is to emphasize the cytotoxic and genotoxic effects of the three food colors at...
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| Format: | Article |
| Language: | English |
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TMKarpinski Publisher, Tomasz M. Karpiński
2022-01-01
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| Series: | European Journal of Biological Research |
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| Online Access: | http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/521 |
| _version_ | 1818948200861532160 |
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| author | Meryem Nassar |
| author_facet | Meryem Nassar |
| author_sort | Meryem Nassar |
| collection | DOAJ |
| description | Food colors such as tartrazine (E102), ammonia caramel (E150c), sulphite ammonia caramel (E150d) are widely used in the food sector. These additives are thought to be a long-term toxicity source. The goal of our research is to emphasize the cytotoxic and genotoxic effects of the three food colors at various concentrations (0.5 %, 1 %, and 2 %) using Allium cepa test. The species is thought to be one of the best for assessing genotoxicity because of its low chromosomal number and lengthy chromosomes. The findings revealed that the three dyes have a cytotoxic impact, as seen by root growth inhibition after 120 h of incubation. The three food dyes had a genotoxic effect, as measured by a decrease in mitotic index and an increase in the frequency of chromosomal aberrations such as chromosomal bridge, stickiness, and vagrant chromosomes, at both concentrations 0.5% and 1%. At 2 %, the mitotic index was reported as 0 and several cytological abnormalities (binucleate and micronucleated cells and fragmented nuclei) were noted. However, further in vitro and in vivo cytogenetic experiments treating cytotoxicity and genotoxicity of the three food dyes using alternative test models (animals, cell lines) will be needed to better understand their mechanisms of action.
DOI: http://dx.doi.org/10.5281/zenodo.5831912 |
| first_indexed | 2024-12-20T08:43:02Z |
| format | Article |
| id | doaj.art-a5f8aa1e03d64806adce70b632d20062 |
| institution | Directory Open Access Journal |
| issn | 2449-8955 |
| language | English |
| last_indexed | 2024-12-20T08:43:02Z |
| publishDate | 2022-01-01 |
| publisher | TMKarpinski Publisher, Tomasz M. Karpiński |
| record_format | Article |
| series | European Journal of Biological Research |
| spelling | doaj.art-a5f8aa1e03d64806adce70b632d200622022-12-21T19:46:19ZengTMKarpinski Publisher, Tomasz M. KarpińskiEuropean Journal of Biological Research2449-89552022-01-01121Cytological and chromosomal damages induced by tartrazine and two classes (III and IV) of caramel food dyesMeryem Nassar0University of 20 Aout 1955, Department of Natural Science and Life BP 26, Route d'El Hadaiek-Skikda 21000, Algeria; Laboratory of Research in Biodiversity Interaction, Ecosystem and Biotechnology ‘LRIBEB’, University of 20 Auguest 1955 BP 26, Route d'El Hadaiek-Skikda 21000, AlgeriaFood colors such as tartrazine (E102), ammonia caramel (E150c), sulphite ammonia caramel (E150d) are widely used in the food sector. These additives are thought to be a long-term toxicity source. The goal of our research is to emphasize the cytotoxic and genotoxic effects of the three food colors at various concentrations (0.5 %, 1 %, and 2 %) using Allium cepa test. The species is thought to be one of the best for assessing genotoxicity because of its low chromosomal number and lengthy chromosomes. The findings revealed that the three dyes have a cytotoxic impact, as seen by root growth inhibition after 120 h of incubation. The three food dyes had a genotoxic effect, as measured by a decrease in mitotic index and an increase in the frequency of chromosomal aberrations such as chromosomal bridge, stickiness, and vagrant chromosomes, at both concentrations 0.5% and 1%. At 2 %, the mitotic index was reported as 0 and several cytological abnormalities (binucleate and micronucleated cells and fragmented nuclei) were noted. However, further in vitro and in vivo cytogenetic experiments treating cytotoxicity and genotoxicity of the three food dyes using alternative test models (animals, cell lines) will be needed to better understand their mechanisms of action. DOI: http://dx.doi.org/10.5281/zenodo.5831912http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/521TartrazineAmmonia caramelSulphite ammonia caramelCytotoxicityGenotoxicity |
| spellingShingle | Meryem Nassar Cytological and chromosomal damages induced by tartrazine and two classes (III and IV) of caramel food dyes European Journal of Biological Research Tartrazine Ammonia caramel Sulphite ammonia caramel Cytotoxicity Genotoxicity |
| title | Cytological and chromosomal damages induced by tartrazine and two classes (III and IV) of caramel food dyes |
| title_full | Cytological and chromosomal damages induced by tartrazine and two classes (III and IV) of caramel food dyes |
| title_fullStr | Cytological and chromosomal damages induced by tartrazine and two classes (III and IV) of caramel food dyes |
| title_full_unstemmed | Cytological and chromosomal damages induced by tartrazine and two classes (III and IV) of caramel food dyes |
| title_short | Cytological and chromosomal damages induced by tartrazine and two classes (III and IV) of caramel food dyes |
| title_sort | cytological and chromosomal damages induced by tartrazine and two classes iii and iv of caramel food dyes |
| topic | Tartrazine Ammonia caramel Sulphite ammonia caramel Cytotoxicity Genotoxicity |
| url | http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/521 |
| work_keys_str_mv | AT meryemnassar cytologicalandchromosomaldamagesinducedbytartrazineandtwoclassesiiiandivofcaramelfooddyes |