Silencing a Simple Extracellular Leucine-Rich Repeat Gene <i>OsI-BAK1</i> Enhances the Resistance of Rice to Brown Planthopper <i>Nilaparvata lugens</i>
Many plant proteins with extracellular leucine-rich repeat (eLRR) domains play an important role in plant immunity. However, the role of one class of eLRR plant proteins—the simple eLRR proteins—in plant defenses against herbivores remains largely unknown. Here, we found that a simple eLRR protein O...
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MDPI AG
2021-11-01
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| Series: | International Journal of Molecular Sciences |
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| author | Miaofen Ye Peng Kuai Shuting Chen Na Lin Meng Ye Lingfei Hu Yonggen Lou |
| author_facet | Miaofen Ye Peng Kuai Shuting Chen Na Lin Meng Ye Lingfei Hu Yonggen Lou |
| author_sort | Miaofen Ye |
| collection | DOAJ |
| description | Many plant proteins with extracellular leucine-rich repeat (eLRR) domains play an important role in plant immunity. However, the role of one class of eLRR plant proteins—the simple eLRR proteins—in plant defenses against herbivores remains largely unknown. Here, we found that a simple eLRR protein OsI-BAK1 in rice localizes to the plasma membrane. Its expression was induced by mechanical wounding, the infestation of gravid females of brown planthopper (BPH) <i>Nilaparvata lugens</i> or white-backed planthopper <i>Sogatella furcifera</i> and treatment with methyl jasmonate or abscisic acid. Silencing <i>OsI-BAK1</i> (ir-<i>ibak1</i>) in rice enhanced the BPH-induced transcript levels of three defense-related WRKY genes (<i>OsWRKY24</i>, <i>OsWRKY53</i> and <i>OsWRKY70</i>) but decreased the induced levels of ethylene. Bioassays revealed that the hatching rate was significantly lower in BPH eggs laid on ir-<i>ibak1</i> plants than wild-type (WT) plants; moreover, gravid BPH females preferred to oviposit on WT plants over ir-<i>ibak1</i> plants. The exogenous application of ethephon on ir-<i>ibak1</i> plants eliminated the BPH oviposition preference between WT and ir-<i>ibak1</i> plants but had no effect on the hatching rate of BPH eggs. These findings suggest that OsI-BAK1 acts as a negative modulator of defense responses in rice to BPH and that BPH might exploit this modulator for its own benefit. |
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| language | English |
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| spelling | doaj.art-a5fa5d25ca144a8691b56a8288ec4d0f2023-11-22T23:38:19ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-11-0122221218210.3390/ijms222212182Silencing a Simple Extracellular Leucine-Rich Repeat Gene <i>OsI-BAK1</i> Enhances the Resistance of Rice to Brown Planthopper <i>Nilaparvata lugens</i>Miaofen Ye0Peng Kuai1Shuting Chen2Na Lin3Meng Ye4Lingfei Hu5Yonggen Lou6State Key Laboratory of Rice Biology & Ministry of Agriculture Key Lab of Agricultural Entomology, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, ChinaState Key Laboratory of Rice Biology & Ministry of Agriculture Key Lab of Agricultural Entomology, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, ChinaState Key Laboratory of Rice Biology & Ministry of Agriculture Key Lab of Agricultural Entomology, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, ChinaState Key Laboratory of Rice Biology & Ministry of Agriculture Key Lab of Agricultural Entomology, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, ChinaState Key Laboratory of Rice Biology & Ministry of Agriculture Key Lab of Agricultural Entomology, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, ChinaState Key Laboratory of Rice Biology & Ministry of Agriculture Key Lab of Agricultural Entomology, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, ChinaState Key Laboratory of Rice Biology & Ministry of Agriculture Key Lab of Agricultural Entomology, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, ChinaMany plant proteins with extracellular leucine-rich repeat (eLRR) domains play an important role in plant immunity. However, the role of one class of eLRR plant proteins—the simple eLRR proteins—in plant defenses against herbivores remains largely unknown. Here, we found that a simple eLRR protein OsI-BAK1 in rice localizes to the plasma membrane. Its expression was induced by mechanical wounding, the infestation of gravid females of brown planthopper (BPH) <i>Nilaparvata lugens</i> or white-backed planthopper <i>Sogatella furcifera</i> and treatment with methyl jasmonate or abscisic acid. Silencing <i>OsI-BAK1</i> (ir-<i>ibak1</i>) in rice enhanced the BPH-induced transcript levels of three defense-related WRKY genes (<i>OsWRKY24</i>, <i>OsWRKY53</i> and <i>OsWRKY70</i>) but decreased the induced levels of ethylene. Bioassays revealed that the hatching rate was significantly lower in BPH eggs laid on ir-<i>ibak1</i> plants than wild-type (WT) plants; moreover, gravid BPH females preferred to oviposit on WT plants over ir-<i>ibak1</i> plants. The exogenous application of ethephon on ir-<i>ibak1</i> plants eliminated the BPH oviposition preference between WT and ir-<i>ibak1</i> plants but had no effect on the hatching rate of BPH eggs. These findings suggest that OsI-BAK1 acts as a negative modulator of defense responses in rice to BPH and that BPH might exploit this modulator for its own benefit.https://www.mdpi.com/1422-0067/22/22/12182rice<i>OsI-BAK1</i><i>Nilaparvata lugens</i>ethyleneplant defense responsessimple extracellular leucine-rich repeat protein |
| spellingShingle | Miaofen Ye Peng Kuai Shuting Chen Na Lin Meng Ye Lingfei Hu Yonggen Lou Silencing a Simple Extracellular Leucine-Rich Repeat Gene <i>OsI-BAK1</i> Enhances the Resistance of Rice to Brown Planthopper <i>Nilaparvata lugens</i> International Journal of Molecular Sciences rice <i>OsI-BAK1</i> <i>Nilaparvata lugens</i> ethylene plant defense responses simple extracellular leucine-rich repeat protein |
| title | Silencing a Simple Extracellular Leucine-Rich Repeat Gene <i>OsI-BAK1</i> Enhances the Resistance of Rice to Brown Planthopper <i>Nilaparvata lugens</i> |
| title_full | Silencing a Simple Extracellular Leucine-Rich Repeat Gene <i>OsI-BAK1</i> Enhances the Resistance of Rice to Brown Planthopper <i>Nilaparvata lugens</i> |
| title_fullStr | Silencing a Simple Extracellular Leucine-Rich Repeat Gene <i>OsI-BAK1</i> Enhances the Resistance of Rice to Brown Planthopper <i>Nilaparvata lugens</i> |
| title_full_unstemmed | Silencing a Simple Extracellular Leucine-Rich Repeat Gene <i>OsI-BAK1</i> Enhances the Resistance of Rice to Brown Planthopper <i>Nilaparvata lugens</i> |
| title_short | Silencing a Simple Extracellular Leucine-Rich Repeat Gene <i>OsI-BAK1</i> Enhances the Resistance of Rice to Brown Planthopper <i>Nilaparvata lugens</i> |
| title_sort | silencing a simple extracellular leucine rich repeat gene i osi bak1 i enhances the resistance of rice to brown planthopper i nilaparvata lugens i |
| topic | rice <i>OsI-BAK1</i> <i>Nilaparvata lugens</i> ethylene plant defense responses simple extracellular leucine-rich repeat protein |
| url | https://www.mdpi.com/1422-0067/22/22/12182 |
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