Inoculation of Arabidopsis seedlings with Ralstonia solanacearum in sterile agar plates
Summary: Ralstonia solanacearum invades plants through their roots and causes devastating bacterial diseases in multiple crops. Here, we present a versatile inoculation assay in Arabidopsis thaliana seedlings grown in sterile agar plates. We describe steps for plant preparation, bacterial inoculatio...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-09-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166723004410 |
| _version_ | 1797769793701412864 |
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| author | Gang Yu Lu Zhang Keke Wang Alberto P. Macho |
| author_facet | Gang Yu Lu Zhang Keke Wang Alberto P. Macho |
| author_sort | Gang Yu |
| collection | DOAJ |
| description | Summary: Ralstonia solanacearum invades plants through their roots and causes devastating bacterial diseases in multiple crops. Here, we present a versatile inoculation assay in Arabidopsis thaliana seedlings grown in sterile agar plates. We describe steps for plant preparation, bacterial inoculation, tissue sampling, and bacterial quantification. This protocol can be used for accurate assessment of bacterial colonization and observation of plant response to infection.For complete details on the use and execution of this protocol, please refer to Dindas et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-03-12T21:14:07Z |
| format | Article |
| id | doaj.art-a5fe35fca57348518b26235cd1fc3b28 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-03-12T21:14:07Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-a5fe35fca57348518b26235cd1fc3b282023-07-30T04:22:57ZengElsevierSTAR Protocols2666-16672023-09-0143102474Inoculation of Arabidopsis seedlings with Ralstonia solanacearum in sterile agar platesGang Yu0Lu Zhang1Keke Wang2Alberto P. Macho3Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 201602, China; School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China; Corresponding authorShanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 201602, China; University of Chinese Academy of Sciences, Beijing 100049, ChinaShanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 201602, ChinaShanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 201602, China; Corresponding authorSummary: Ralstonia solanacearum invades plants through their roots and causes devastating bacterial diseases in multiple crops. Here, we present a versatile inoculation assay in Arabidopsis thaliana seedlings grown in sterile agar plates. We describe steps for plant preparation, bacterial inoculation, tissue sampling, and bacterial quantification. This protocol can be used for accurate assessment of bacterial colonization and observation of plant response to infection.For complete details on the use and execution of this protocol, please refer to Dindas et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166723004410MicrobiologyModel OrganismsPlant Sciences |
| spellingShingle | Gang Yu Lu Zhang Keke Wang Alberto P. Macho Inoculation of Arabidopsis seedlings with Ralstonia solanacearum in sterile agar plates STAR Protocols Microbiology Model Organisms Plant Sciences |
| title | Inoculation of Arabidopsis seedlings with Ralstonia solanacearum in sterile agar plates |
| title_full | Inoculation of Arabidopsis seedlings with Ralstonia solanacearum in sterile agar plates |
| title_fullStr | Inoculation of Arabidopsis seedlings with Ralstonia solanacearum in sterile agar plates |
| title_full_unstemmed | Inoculation of Arabidopsis seedlings with Ralstonia solanacearum in sterile agar plates |
| title_short | Inoculation of Arabidopsis seedlings with Ralstonia solanacearum in sterile agar plates |
| title_sort | inoculation of arabidopsis seedlings with ralstonia solanacearum in sterile agar plates |
| topic | Microbiology Model Organisms Plant Sciences |
| url | http://www.sciencedirect.com/science/article/pii/S2666166723004410 |
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