Nose-to-Brain Translocation and Cerebral Biodegradation of Thin Graphene Oxide Nanosheets
Summary: Understanding the interactions of graphene oxide (GO)-based materials with biological systems is critical due to the potential applications of these materials. Here, we investigate the extent to which single- to few-layer GO sheets of different controlled lateral dimensions translocate from...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-09-01
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| Series: | Cell Reports Physical Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666386420301879 |
| _version_ | 1818346224897490944 |
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| author | Leon Newman Artur Filipe Rodrigues Dhifaf A. Jasim Isabella Anna Vacchi Cécilia Ménard-Moyon Alberto Bianco Cyrill Bussy Kostas Kostarelos |
| author_facet | Leon Newman Artur Filipe Rodrigues Dhifaf A. Jasim Isabella Anna Vacchi Cécilia Ménard-Moyon Alberto Bianco Cyrill Bussy Kostas Kostarelos |
| author_sort | Leon Newman |
| collection | DOAJ |
| description | Summary: Understanding the interactions of graphene oxide (GO)-based materials with biological systems is critical due to the potential applications of these materials. Here, we investigate the extent to which single- to few-layer GO sheets of different controlled lateral dimensions translocate from the nose to the brain following intranasal instillation. We explore tissue location and in vivo biodegradability of the translocated materials using various techniques. Mass spectrometry and confocal Raman analyses indicate that trace amounts of GO undergo nose-to-brain translocation in a size-dependent manner. The smallest GO-sheet size category (us-GO, 10-550 nm) gains the greatest access to the brain in terms of quantity and coverage. Confocal Raman mapping and immunofluorescence combinations show that in vivo, us-GO resides in association with microglia. Point-and-shoot Raman spectroscopy shows that trace quantities of us-GO are maintained over 1 month, but undergo biodegradation-related changes. This study adds to growing awareness regarding the fate of graphene-based materials in biological systems. |
| first_indexed | 2024-12-13T17:14:53Z |
| format | Article |
| id | doaj.art-a600568fd8b148ecb89f02baecea2780 |
| institution | Directory Open Access Journal |
| issn | 2666-3864 |
| language | English |
| last_indexed | 2024-12-13T17:14:53Z |
| publishDate | 2020-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports Physical Science |
| spelling | doaj.art-a600568fd8b148ecb89f02baecea27802022-12-21T23:37:27ZengElsevierCell Reports Physical Science2666-38642020-09-0119100176Nose-to-Brain Translocation and Cerebral Biodegradation of Thin Graphene Oxide NanosheetsLeon Newman0Artur Filipe Rodrigues1Dhifaf A. Jasim2Isabella Anna Vacchi3Cécilia Ménard-Moyon4Alberto Bianco5Cyrill Bussy6Kostas Kostarelos7Nanomedicine Lab, Faculty of Biology, Medicine, and Health, The University of Manchester, AV Hill Building, Manchester, UK; National Graphene Institute, The University of Manchester, Manchester, UKNanomedicine Lab, Faculty of Biology, Medicine, and Health, The University of Manchester, AV Hill Building, Manchester, UK; National Graphene Institute, The University of Manchester, Manchester, UKNanomedicine Lab, Faculty of Biology, Medicine, and Health, The University of Manchester, AV Hill Building, Manchester, UK; National Graphene Institute, The University of Manchester, Manchester, UKCNRS, Immunology, Immunopathology and Therapeutic Chemistry, UPR3572, University of Strasbourg, ISIS, 67000 Strasbourg, FranceCNRS, Immunology, Immunopathology and Therapeutic Chemistry, UPR3572, University of Strasbourg, ISIS, 67000 Strasbourg, FranceCNRS, Immunology, Immunopathology and Therapeutic Chemistry, UPR3572, University of Strasbourg, ISIS, 67000 Strasbourg, FranceNanomedicine Lab, Faculty of Biology, Medicine, and Health, The University of Manchester, AV Hill Building, Manchester, UK; National Graphene Institute, The University of Manchester, Manchester, UK; Corresponding authorNanomedicine Lab, Faculty of Biology, Medicine, and Health, The University of Manchester, AV Hill Building, Manchester, UK; National Graphene Institute, The University of Manchester, Manchester, UK; Catalan Institute of Nanoscience and Nanotechnology (ICN2) and The Barcelona Institute of Science and Technology (BIST), Campus UAB, Bellaterra, Barcelona, Spain; Corresponding authorSummary: Understanding the interactions of graphene oxide (GO)-based materials with biological systems is critical due to the potential applications of these materials. Here, we investigate the extent to which single- to few-layer GO sheets of different controlled lateral dimensions translocate from the nose to the brain following intranasal instillation. We explore tissue location and in vivo biodegradability of the translocated materials using various techniques. Mass spectrometry and confocal Raman analyses indicate that trace amounts of GO undergo nose-to-brain translocation in a size-dependent manner. The smallest GO-sheet size category (us-GO, 10-550 nm) gains the greatest access to the brain in terms of quantity and coverage. Confocal Raman mapping and immunofluorescence combinations show that in vivo, us-GO resides in association with microglia. Point-and-shoot Raman spectroscopy shows that trace quantities of us-GO are maintained over 1 month, but undergo biodegradation-related changes. This study adds to growing awareness regarding the fate of graphene-based materials in biological systems.http://www.sciencedirect.com/science/article/pii/S2666386420301879graphene oxidenose-to-brain translocationbraininhalation2D materialsbiodegradation |
| spellingShingle | Leon Newman Artur Filipe Rodrigues Dhifaf A. Jasim Isabella Anna Vacchi Cécilia Ménard-Moyon Alberto Bianco Cyrill Bussy Kostas Kostarelos Nose-to-Brain Translocation and Cerebral Biodegradation of Thin Graphene Oxide Nanosheets Cell Reports Physical Science graphene oxide nose-to-brain translocation brain inhalation 2D materials biodegradation |
| title | Nose-to-Brain Translocation and Cerebral Biodegradation of Thin Graphene Oxide Nanosheets |
| title_full | Nose-to-Brain Translocation and Cerebral Biodegradation of Thin Graphene Oxide Nanosheets |
| title_fullStr | Nose-to-Brain Translocation and Cerebral Biodegradation of Thin Graphene Oxide Nanosheets |
| title_full_unstemmed | Nose-to-Brain Translocation and Cerebral Biodegradation of Thin Graphene Oxide Nanosheets |
| title_short | Nose-to-Brain Translocation and Cerebral Biodegradation of Thin Graphene Oxide Nanosheets |
| title_sort | nose to brain translocation and cerebral biodegradation of thin graphene oxide nanosheets |
| topic | graphene oxide nose-to-brain translocation brain inhalation 2D materials biodegradation |
| url | http://www.sciencedirect.com/science/article/pii/S2666386420301879 |
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