3D Hepatic Organoid-Based Advancements in LIVER Tissue Engineering
Liver-associated diseases and tissue engineering approaches based on in vitro culture of functional Primary human hepatocytes (PHH) had been restricted by the rapid de-differentiation in 2D culture conditions which restricted their usability. It was proven that cells growing in 3D format can better...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-11-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/8/11/185 |
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| author | Amit Panwar Prativa Das Lay Poh Tan |
| author_facet | Amit Panwar Prativa Das Lay Poh Tan |
| author_sort | Amit Panwar |
| collection | DOAJ |
| description | Liver-associated diseases and tissue engineering approaches based on in vitro culture of functional Primary human hepatocytes (PHH) had been restricted by the rapid de-differentiation in 2D culture conditions which restricted their usability. It was proven that cells growing in 3D format can better mimic the in vivo microenvironment, and thus help in maintaining metabolic activity, phenotypic properties, and longevity of the in vitro cultures. Again, the culture method and type of cell population are also recognized as important parameters for functional maintenance of primary hepatocytes. Hepatic organoids formed by self-assembly of hepatic cells are microtissues, and were able to show long-term in vitro maintenance of hepato-specific characteristics. Thus, hepatic organoids were recognized as an effective tool for screening potential cures and modeling liver diseases effectively. The current review summarizes the importance of 3D hepatic organoid culture over other conventional 2D and 3D culture models and its applicability in Liver tissue engineering. |
| first_indexed | 2024-03-10T05:42:27Z |
| format | Article |
| id | doaj.art-a602ace351044574b942e5322d2efe0f |
| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-10T05:42:27Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj.art-a602ace351044574b942e5322d2efe0f2023-11-22T22:26:50ZengMDPI AGBioengineering2306-53542021-11-0181118510.3390/bioengineering81101853D Hepatic Organoid-Based Advancements in LIVER Tissue EngineeringAmit Panwar0Prativa Das1Lay Poh Tan2School of Materials Science & Engineering, Nanyang Technological University, Singapore 639798, SingaporeThe Henry Samueli School of Engineering, University of California, Irvine, CA 92617, USASchool of Materials Science & Engineering, Nanyang Technological University, Singapore 639798, SingaporeLiver-associated diseases and tissue engineering approaches based on in vitro culture of functional Primary human hepatocytes (PHH) had been restricted by the rapid de-differentiation in 2D culture conditions which restricted their usability. It was proven that cells growing in 3D format can better mimic the in vivo microenvironment, and thus help in maintaining metabolic activity, phenotypic properties, and longevity of the in vitro cultures. Again, the culture method and type of cell population are also recognized as important parameters for functional maintenance of primary hepatocytes. Hepatic organoids formed by self-assembly of hepatic cells are microtissues, and were able to show long-term in vitro maintenance of hepato-specific characteristics. Thus, hepatic organoids were recognized as an effective tool for screening potential cures and modeling liver diseases effectively. The current review summarizes the importance of 3D hepatic organoid culture over other conventional 2D and 3D culture models and its applicability in Liver tissue engineering.https://www.mdpi.com/2306-5354/8/11/185liver tissue engineeringhepatic organoidshepatic functionhepatocytes3D culture |
| spellingShingle | Amit Panwar Prativa Das Lay Poh Tan 3D Hepatic Organoid-Based Advancements in LIVER Tissue Engineering Bioengineering liver tissue engineering hepatic organoids hepatic function hepatocytes 3D culture |
| title | 3D Hepatic Organoid-Based Advancements in LIVER Tissue Engineering |
| title_full | 3D Hepatic Organoid-Based Advancements in LIVER Tissue Engineering |
| title_fullStr | 3D Hepatic Organoid-Based Advancements in LIVER Tissue Engineering |
| title_full_unstemmed | 3D Hepatic Organoid-Based Advancements in LIVER Tissue Engineering |
| title_short | 3D Hepatic Organoid-Based Advancements in LIVER Tissue Engineering |
| title_sort | 3d hepatic organoid based advancements in liver tissue engineering |
| topic | liver tissue engineering hepatic organoids hepatic function hepatocytes 3D culture |
| url | https://www.mdpi.com/2306-5354/8/11/185 |
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