Non-targeted metabolomics of cooked cowpea (Vigna unguiculata) and pigeon pea (Cajanus cajan) from Ghana using two distinct and complementary analytical platforms
Legumes are global staple foods with multiple human health properties that merit detailed composition analysis in cooked forms. This study analyzed cowpea [Vigna unguiculata] (three varieties: Dagbantuya, Sangyi, and Tukara), pigeon pea [Cajanus cajan], and common bean [Phaseolus vulgaris] using two...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-07-01
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| Series: | Food Chemistry: Molecular Sciences |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666566222000156 |
| _version_ | 1819283813325340672 |
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| author | Brooke Sayre-Chavez Bridget Baxter Corey D. Broeckling María Muñoz-Amatriaín Mark Manary Elizabeth P. Ryan |
| author_facet | Brooke Sayre-Chavez Bridget Baxter Corey D. Broeckling María Muñoz-Amatriaín Mark Manary Elizabeth P. Ryan |
| author_sort | Brooke Sayre-Chavez |
| collection | DOAJ |
| description | Legumes are global staple foods with multiple human health properties that merit detailed composition analysis in cooked forms. This study analyzed cowpea [Vigna unguiculata] (three varieties: Dagbantuya, Sangyi, and Tukara), pigeon pea [Cajanus cajan], and common bean [Phaseolus vulgaris] using two distinct ultra-performance liquid chromatography mass spectrometry (UPLC-MS) platforms and analytical workflows. Comparisons between cowpea and pigeon pea consumed in Ghana, and common bean (navy bean) from USA, revealed 75 metabolites that differentiated cowpeas. Metabolite fold-change comparisons resulted in 142 metabolites with significantly higher abundance in cowpea, and 154 higher in abundance from pigeon pea. 3-(all-trans-nonaprenyl)benzene-1,2-diol, N-tetracosanoylphytosphingosine, and sitoindoside II are novel identifications in cowpea, with notably higher abundance than other legumes tested. Cowpea variety specific markers were tonkinelin (Dagbantuya), pheophytin A (Sangyi), and linoleoyl ethanolamide (Tukara). This study identified novel cowpea and pigeon pea food metabolites that warrant continued investigation as bioactive food components following consumption in people. |
| first_indexed | 2024-12-24T01:37:27Z |
| format | Article |
| id | doaj.art-a6032ef5f6cf4ccf8dbb57ffcbfb8663 |
| institution | Directory Open Access Journal |
| issn | 2666-5662 |
| language | English |
| last_indexed | 2024-12-24T01:37:27Z |
| publishDate | 2022-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: Molecular Sciences |
| spelling | doaj.art-a6032ef5f6cf4ccf8dbb57ffcbfb86632022-12-21T17:22:09ZengElsevierFood Chemistry: Molecular Sciences2666-56622022-07-014100087Non-targeted metabolomics of cooked cowpea (Vigna unguiculata) and pigeon pea (Cajanus cajan) from Ghana using two distinct and complementary analytical platformsBrooke Sayre-Chavez0Bridget Baxter1Corey D. Broeckling2María Muñoz-Amatriaín3Mark Manary4Elizabeth P. Ryan5Department of Soil and Crop Sciences, Colorado State University, Fort Collins, CO 80523, USADepartment of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, CO 80523, USAAnalytical Resources Core: Bioanalysis and Omics Center, Colorado State University, Fort Collins, CO 80523, USADepartment of Soil and Crop Sciences, Colorado State University, Fort Collins, CO 80523, USA; Department of Molecular Biology, University of León, 24071 León, SpainDepartment of Pediatrics, Washington University School of Medicine in St. Louis, St. Louis, MO 63110, USADepartment of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, CO 80523, USA; Corresponding author at: Department of Environmental and Radiological Health Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University/Colorado School of Public Health, 1680 Campus Delivery, 200 W. Lake Street, Fort Collins, CO 80523-1680, USA.Legumes are global staple foods with multiple human health properties that merit detailed composition analysis in cooked forms. This study analyzed cowpea [Vigna unguiculata] (three varieties: Dagbantuya, Sangyi, and Tukara), pigeon pea [Cajanus cajan], and common bean [Phaseolus vulgaris] using two distinct ultra-performance liquid chromatography mass spectrometry (UPLC-MS) platforms and analytical workflows. Comparisons between cowpea and pigeon pea consumed in Ghana, and common bean (navy bean) from USA, revealed 75 metabolites that differentiated cowpeas. Metabolite fold-change comparisons resulted in 142 metabolites with significantly higher abundance in cowpea, and 154 higher in abundance from pigeon pea. 3-(all-trans-nonaprenyl)benzene-1,2-diol, N-tetracosanoylphytosphingosine, and sitoindoside II are novel identifications in cowpea, with notably higher abundance than other legumes tested. Cowpea variety specific markers were tonkinelin (Dagbantuya), pheophytin A (Sangyi), and linoleoyl ethanolamide (Tukara). This study identified novel cowpea and pigeon pea food metabolites that warrant continued investigation as bioactive food components following consumption in people.http://www.sciencedirect.com/science/article/pii/S2666566222000156Vigna unguiculataCajanus cajanPhaseolus vulgarisNon-targeted metabolomicsUPLC-MSLegumes |
| spellingShingle | Brooke Sayre-Chavez Bridget Baxter Corey D. Broeckling María Muñoz-Amatriaín Mark Manary Elizabeth P. Ryan Non-targeted metabolomics of cooked cowpea (Vigna unguiculata) and pigeon pea (Cajanus cajan) from Ghana using two distinct and complementary analytical platforms Food Chemistry: Molecular Sciences Vigna unguiculata Cajanus cajan Phaseolus vulgaris Non-targeted metabolomics UPLC-MS Legumes |
| title | Non-targeted metabolomics of cooked cowpea (Vigna unguiculata) and pigeon pea (Cajanus cajan) from Ghana using two distinct and complementary analytical platforms |
| title_full | Non-targeted metabolomics of cooked cowpea (Vigna unguiculata) and pigeon pea (Cajanus cajan) from Ghana using two distinct and complementary analytical platforms |
| title_fullStr | Non-targeted metabolomics of cooked cowpea (Vigna unguiculata) and pigeon pea (Cajanus cajan) from Ghana using two distinct and complementary analytical platforms |
| title_full_unstemmed | Non-targeted metabolomics of cooked cowpea (Vigna unguiculata) and pigeon pea (Cajanus cajan) from Ghana using two distinct and complementary analytical platforms |
| title_short | Non-targeted metabolomics of cooked cowpea (Vigna unguiculata) and pigeon pea (Cajanus cajan) from Ghana using two distinct and complementary analytical platforms |
| title_sort | non targeted metabolomics of cooked cowpea vigna unguiculata and pigeon pea cajanus cajan from ghana using two distinct and complementary analytical platforms |
| topic | Vigna unguiculata Cajanus cajan Phaseolus vulgaris Non-targeted metabolomics UPLC-MS Legumes |
| url | http://www.sciencedirect.com/science/article/pii/S2666566222000156 |
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