Shielding of the geomagnetic field reduces hydrogen peroxide production in human neuroblastoma cell and inhibits the activity of CuZn superoxide dismutase
Abstract Accumulative evidence has shown the adverse effects of a geomagnetic field shielded condition, so called a hypomagnetic field (HMF), on the metabolic processes and oxidative stress in animals and cells. However, the underlying mechanism remains unclear. In this study, we evaluate the role o...
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| Format: | Article |
| Language: | English |
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Oxford University Press
2017-04-01
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| Series: | Protein & Cell |
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| Online Access: | http://link.springer.com/article/10.1007/s13238-017-0403-9 |
| _version_ | 1797756992532512768 |
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| author | Hai-tao Zhang Zi-jian Zhang Wei-chuan Mo Ping-dong Hu Hai-min Ding Ying Liu Qian Hua Rong-qiao He |
| author_facet | Hai-tao Zhang Zi-jian Zhang Wei-chuan Mo Ping-dong Hu Hai-min Ding Ying Liu Qian Hua Rong-qiao He |
| author_sort | Hai-tao Zhang |
| collection | DOAJ |
| description | Abstract Accumulative evidence has shown the adverse effects of a geomagnetic field shielded condition, so called a hypomagnetic field (HMF), on the metabolic processes and oxidative stress in animals and cells. However, the underlying mechanism remains unclear. In this study, we evaluate the role of HMF on the regulation of cellular reactive oxygen species (ROS) in human neuroblastoma SH-SY5Y cells. We found that HMF exposure led to ROS decrease, and that restoring the decrease by additional H2O2 rescued the HMF-enhanced cell proliferation. The measurements on ROS related indexes, including total anti-oxidant capacity, H2O2 and superoxide anion levels, and superoxide dismutase (SOD) activity and expression, indicated that the HMF reduced H2O2 production and inhibited the activity of CuZn-SOD. Moreover, the HMF accelerated the denaturation of CuZn-SOD as well as enhanced aggregation of CuZn-SOD protein, in vitro. Our findings indicate that CuZn-SOD is able to response to the HMF stress and suggest it a mediator of the HMF effect. |
| first_indexed | 2024-03-12T18:09:34Z |
| format | Article |
| id | doaj.art-a605aac30f7f4c42b564c453a81d8d96 |
| institution | Directory Open Access Journal |
| issn | 1674-800X 1674-8018 |
| language | English |
| last_indexed | 2024-03-12T18:09:34Z |
| publishDate | 2017-04-01 |
| publisher | Oxford University Press |
| record_format | Article |
| series | Protein & Cell |
| spelling | doaj.art-a605aac30f7f4c42b564c453a81d8d962023-08-02T09:17:48ZengOxford University PressProtein & Cell1674-800X1674-80182017-04-018752753710.1007/s13238-017-0403-9Shielding of the geomagnetic field reduces hydrogen peroxide production in human neuroblastoma cell and inhibits the activity of CuZn superoxide dismutaseHai-tao Zhang0Zi-jian Zhang1Wei-chuan Mo2Ping-dong Hu3Hai-min Ding4Ying Liu5Qian Hua6Rong-qiao He7State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of SciencesState Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of SciencesState Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of SciencesState Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of SciencesInstitute of Beijing Chinese Traditional Medicine, Beijing University of Chinese MedicineState Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of SciencesInstitute of Beijing Chinese Traditional Medicine, Beijing University of Chinese MedicineState Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of SciencesAbstract Accumulative evidence has shown the adverse effects of a geomagnetic field shielded condition, so called a hypomagnetic field (HMF), on the metabolic processes and oxidative stress in animals and cells. However, the underlying mechanism remains unclear. In this study, we evaluate the role of HMF on the regulation of cellular reactive oxygen species (ROS) in human neuroblastoma SH-SY5Y cells. We found that HMF exposure led to ROS decrease, and that restoring the decrease by additional H2O2 rescued the HMF-enhanced cell proliferation. The measurements on ROS related indexes, including total anti-oxidant capacity, H2O2 and superoxide anion levels, and superoxide dismutase (SOD) activity and expression, indicated that the HMF reduced H2O2 production and inhibited the activity of CuZn-SOD. Moreover, the HMF accelerated the denaturation of CuZn-SOD as well as enhanced aggregation of CuZn-SOD protein, in vitro. Our findings indicate that CuZn-SOD is able to response to the HMF stress and suggest it a mediator of the HMF effect.http://link.springer.com/article/10.1007/s13238-017-0403-9hypomagnetic fieldreactive oxygen specieshydrogen peroxidesuperoxide dismutaseoxidative stress |
| spellingShingle | Hai-tao Zhang Zi-jian Zhang Wei-chuan Mo Ping-dong Hu Hai-min Ding Ying Liu Qian Hua Rong-qiao He Shielding of the geomagnetic field reduces hydrogen peroxide production in human neuroblastoma cell and inhibits the activity of CuZn superoxide dismutase Protein & Cell hypomagnetic field reactive oxygen species hydrogen peroxide superoxide dismutase oxidative stress |
| title | Shielding of the geomagnetic field reduces hydrogen peroxide production in human neuroblastoma cell and inhibits the activity of CuZn superoxide dismutase |
| title_full | Shielding of the geomagnetic field reduces hydrogen peroxide production in human neuroblastoma cell and inhibits the activity of CuZn superoxide dismutase |
| title_fullStr | Shielding of the geomagnetic field reduces hydrogen peroxide production in human neuroblastoma cell and inhibits the activity of CuZn superoxide dismutase |
| title_full_unstemmed | Shielding of the geomagnetic field reduces hydrogen peroxide production in human neuroblastoma cell and inhibits the activity of CuZn superoxide dismutase |
| title_short | Shielding of the geomagnetic field reduces hydrogen peroxide production in human neuroblastoma cell and inhibits the activity of CuZn superoxide dismutase |
| title_sort | shielding of the geomagnetic field reduces hydrogen peroxide production in human neuroblastoma cell and inhibits the activity of cuzn superoxide dismutase |
| topic | hypomagnetic field reactive oxygen species hydrogen peroxide superoxide dismutase oxidative stress |
| url | http://link.springer.com/article/10.1007/s13238-017-0403-9 |
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