Effects of the drying method and extraction solvent on antioxidant and anti-inflammatory activity of Melosira nummuloides bioproducts
Abstract Melosira nummuloides is a marine diatom with potential use as food, fuel, and a dietary supplement. However, the efficacy of its extraction and drying techniques have not been explored. Here, M. nummuloides powders were prepared by two drying methods—hot-air drying (HAD) and freeze-drying (...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2023-09-01
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| Series: | Applied Biological Chemistry |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13765-023-00817-y |
| _version_ | 1827708760691310592 |
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| author | Do Manh Cuong Dae Kyeong Kim Meran Keshawa Ediriweera Jong-Eun Park Jeong Yong Moon Somi Kim Cho |
| author_facet | Do Manh Cuong Dae Kyeong Kim Meran Keshawa Ediriweera Jong-Eun Park Jeong Yong Moon Somi Kim Cho |
| author_sort | Do Manh Cuong |
| collection | DOAJ |
| description | Abstract Melosira nummuloides is a marine diatom with potential use as food, fuel, and a dietary supplement. However, the efficacy of its extraction and drying techniques have not been explored. Here, M. nummuloides powders were prepared by two drying methods—hot-air drying (HAD) and freeze-drying (FD)—and extracted with hot water, ethanol, methanol, and chloroform:methanol (CM) at a ratio of 2:1 v/v. The antioxidant and anti-inflammatory activity of each extract was investigated. The CM extract had the greatest 2,2-diphenyl-1-picrylhydrazyl and 2,2ʹ-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity among the solvent extracts, and a slight difference in antioxidant activity was observed across the various drying methods. Compared to other extracts, both the FD-CM and HAD-CM extracts showed stronger anti-inflammatory effects by inhibiting nitric oxide production in lipopolysaccharide-stimulated RAW 264.7 cells. Furthermore, the FD-CM extract contained a wide range of lipophilic compounds. Notably, myristic acid (29.08 ± 0.45 mg/g dry weight powder extract (DW)), oleic acid (25.20 ± 0.92 mg/g DW), palmitoleic acid (10.77 ± 0.41 mg/g DW), eicosapentaenoic acid (12.53 ± 1.00 mg/g DW), neophytadiene (8.42 ± 0.51 mg/g DW), and α-linolenic acid (1.27 ± 0.005 mg/g DW) were among the prominent compounds identified. It is plausible to suggest that the abundance of these lipophilic compounds contributes to the remarkable antioxidative and anti-inflammatory potential exhibited by the FD-CM extract. Our results provide insights into the preferential drying methods and extraction solvents for producing M. nummuloides-based products with enhanced antioxidant and anti-inflammatory activity. |
| first_indexed | 2024-03-10T17:10:23Z |
| format | Article |
| id | doaj.art-a606771692884546b322b5998b538232 |
| institution | Directory Open Access Journal |
| issn | 2468-0842 |
| language | English |
| last_indexed | 2024-03-10T17:10:23Z |
| publishDate | 2023-09-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Applied Biological Chemistry |
| spelling | doaj.art-a606771692884546b322b5998b5382322023-11-20T10:41:16ZengSpringerOpenApplied Biological Chemistry2468-08422023-09-0166111010.1186/s13765-023-00817-yEffects of the drying method and extraction solvent on antioxidant and anti-inflammatory activity of Melosira nummuloides bioproductsDo Manh Cuong0Dae Kyeong Kim1Meran Keshawa Ediriweera2Jong-Eun Park3Jeong Yong Moon4Somi Kim Cho5Interdisciplinary Graduate Program in Advanced Convergence Technology and Science, Jeju National UniversityInterdisciplinary Graduate Program in Advanced Convergence Technology and Science, Jeju National UniversityDepartment of Biochemistry and Molecular Biology, Faculty of Medicine, University of ColomboDepartment of Animal Biotechnology, College of Applied Life Sciences, SARI, Jeju National UniversitySubtropical/Tropical Organism Gene Bank, Jeju National UniversityInterdisciplinary Graduate Program in Advanced Convergence Technology and Science, Jeju National UniversityAbstract Melosira nummuloides is a marine diatom with potential use as food, fuel, and a dietary supplement. However, the efficacy of its extraction and drying techniques have not been explored. Here, M. nummuloides powders were prepared by two drying methods—hot-air drying (HAD) and freeze-drying (FD)—and extracted with hot water, ethanol, methanol, and chloroform:methanol (CM) at a ratio of 2:1 v/v. The antioxidant and anti-inflammatory activity of each extract was investigated. The CM extract had the greatest 2,2-diphenyl-1-picrylhydrazyl and 2,2ʹ-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity among the solvent extracts, and a slight difference in antioxidant activity was observed across the various drying methods. Compared to other extracts, both the FD-CM and HAD-CM extracts showed stronger anti-inflammatory effects by inhibiting nitric oxide production in lipopolysaccharide-stimulated RAW 264.7 cells. Furthermore, the FD-CM extract contained a wide range of lipophilic compounds. Notably, myristic acid (29.08 ± 0.45 mg/g dry weight powder extract (DW)), oleic acid (25.20 ± 0.92 mg/g DW), palmitoleic acid (10.77 ± 0.41 mg/g DW), eicosapentaenoic acid (12.53 ± 1.00 mg/g DW), neophytadiene (8.42 ± 0.51 mg/g DW), and α-linolenic acid (1.27 ± 0.005 mg/g DW) were among the prominent compounds identified. It is plausible to suggest that the abundance of these lipophilic compounds contributes to the remarkable antioxidative and anti-inflammatory potential exhibited by the FD-CM extract. Our results provide insights into the preferential drying methods and extraction solvents for producing M. nummuloides-based products with enhanced antioxidant and anti-inflammatory activity.https://doi.org/10.1186/s13765-023-00817-yAntioxidantAnti-inflammationDrying methodLipophilic compoundMelosira nummuloides |
| spellingShingle | Do Manh Cuong Dae Kyeong Kim Meran Keshawa Ediriweera Jong-Eun Park Jeong Yong Moon Somi Kim Cho Effects of the drying method and extraction solvent on antioxidant and anti-inflammatory activity of Melosira nummuloides bioproducts Applied Biological Chemistry Antioxidant Anti-inflammation Drying method Lipophilic compound Melosira nummuloides |
| title | Effects of the drying method and extraction solvent on antioxidant and anti-inflammatory activity of Melosira nummuloides bioproducts |
| title_full | Effects of the drying method and extraction solvent on antioxidant and anti-inflammatory activity of Melosira nummuloides bioproducts |
| title_fullStr | Effects of the drying method and extraction solvent on antioxidant and anti-inflammatory activity of Melosira nummuloides bioproducts |
| title_full_unstemmed | Effects of the drying method and extraction solvent on antioxidant and anti-inflammatory activity of Melosira nummuloides bioproducts |
| title_short | Effects of the drying method and extraction solvent on antioxidant and anti-inflammatory activity of Melosira nummuloides bioproducts |
| title_sort | effects of the drying method and extraction solvent on antioxidant and anti inflammatory activity of melosira nummuloides bioproducts |
| topic | Antioxidant Anti-inflammation Drying method Lipophilic compound Melosira nummuloides |
| url | https://doi.org/10.1186/s13765-023-00817-y |
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