Utilization of cryopreserved ruminal liquor in in vitro gas production technique for evaluating nutritive value of some feedstuffs
In vitro gas production technique (IVGPT) is a routine method in nutritional sciences to determine energy content, organic matter (OM) digestibility, and fermentation kinetics of feedstuffs. After collecting from two ruminally cannulated Holstein heifers (350 kg), rumen liquors were used either fres...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Kafkas University, Faculty of Veterinary Medicine
2017-03-01
|
| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| Subjects: | |
| Online Access: | https://vetdergikafkas.org/pdf.php?id=2047 |
| _version_ | 1797800584012627968 |
|---|---|
| author | GÜLŞEN N ARIK HD HAYIRLI A ALATAŞ MS AKSOY M |
| author_facet | GÜLŞEN N ARIK HD HAYIRLI A ALATAŞ MS AKSOY M |
| author_sort | GÜLŞEN N |
| collection | DOAJ |
| description | In vitro gas production technique (IVGPT) is a routine method in nutritional sciences to determine energy content, organic matter (OM)
digestibility, and fermentation kinetics of feedstuffs. After collecting from two ruminally cannulated Holstein heifers (350 kg), rumen liquors
were used either fresh or cryopreserved form in the inoculums for IVGPT. Starch- (barley, wheat, and corn) and protein-rich (sunflower meal,
cotton seed meal, and soybean meal) feedstuffs were evaluated for gas production kinetics, fermentation pattern, and energy content
in 5 replicates. pH, NH3-N concentration and volatile fatty acids (VFA) profile, gas production, and fermentation kinetics parameters were
measured. Data were analyzed by 2-way ANOVA. Viable protozoa rate was found to be 70.8% in cryopreserved rumen liquor after thawing.
Decrease in pH in thawed rumen liquor was less than fresh rumen liquor as the incubation period advanced. Utilization of frozen rumen
liquor after thawing in IVGPT was associated with lower VFA and NH3-N concentration, cumulative gas production, and metabolisable energy
estimate for all feedstuffs. In conclusion, despite high correlation between in vitro data obtained from fresh and thawed rumen liquors to
predict gas production, further experiments should cope with improving cryopreservation protocol for rumen liquors in order to optimize
microbial activity for maintaining fermentation pattern. |
| first_indexed | 2024-03-13T04:37:33Z |
| format | Article |
| id | doaj.art-a60a8ee349044394afe627c320e3749d |
| institution | Directory Open Access Journal |
| issn | 1309-2251 |
| language | English |
| last_indexed | 2024-03-13T04:37:33Z |
| publishDate | 2017-03-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj.art-a60a8ee349044394afe627c320e3749d2023-06-19T06:46:03ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512017-03-0123232533210.9775/kvfd.2016.165272047Utilization of cryopreserved ruminal liquor in in vitro gas production technique for evaluating nutritive value of some feedstuffsGÜLŞEN N0ARIK HD1HAYIRLI A2ALATAŞ MS3AKSOY M4Department of Animal Nutrition and Nutritional Disorders, Faculty of Veterinary Medicine, Selcuk University, TR-42100 Konya - TURKEYDepartment of Animal Nutrition and Nutritional Disorders, Faculty of Veterinary Medicine, Selcuk University, TR-42100 Konya - TURKEYDepartment of Animal Nutrition and Nutritional Disorders, Faculty of Veterinary Medicine, Atatürk University, TR-25700 Erzurum - TURKEYDepartment of Animal Nutrition and Nutritional Disorders, Faculty of Veterinary Medicine, Selcuk University, TR-42100 Konya - TURKEYDepartment of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Adnan Menderes University, TR-09500 Aydın - TURKEYIn vitro gas production technique (IVGPT) is a routine method in nutritional sciences to determine energy content, organic matter (OM) digestibility, and fermentation kinetics of feedstuffs. After collecting from two ruminally cannulated Holstein heifers (350 kg), rumen liquors were used either fresh or cryopreserved form in the inoculums for IVGPT. Starch- (barley, wheat, and corn) and protein-rich (sunflower meal, cotton seed meal, and soybean meal) feedstuffs were evaluated for gas production kinetics, fermentation pattern, and energy content in 5 replicates. pH, NH3-N concentration and volatile fatty acids (VFA) profile, gas production, and fermentation kinetics parameters were measured. Data were analyzed by 2-way ANOVA. Viable protozoa rate was found to be 70.8% in cryopreserved rumen liquor after thawing. Decrease in pH in thawed rumen liquor was less than fresh rumen liquor as the incubation period advanced. Utilization of frozen rumen liquor after thawing in IVGPT was associated with lower VFA and NH3-N concentration, cumulative gas production, and metabolisable energy estimate for all feedstuffs. In conclusion, despite high correlation between in vitro data obtained from fresh and thawed rumen liquors to predict gas production, further experiments should cope with improving cryopreservation protocol for rumen liquors in order to optimize microbial activity for maintaining fermentation pattern.https://vetdergikafkas.org/pdf.php?id=2047cryopreserved rumen liquorenergy predictionfermentationgas productionin vitro gas testvolatile fatty acid |
| spellingShingle | GÜLŞEN N ARIK HD HAYIRLI A ALATAŞ MS AKSOY M Utilization of cryopreserved ruminal liquor in in vitro gas production technique for evaluating nutritive value of some feedstuffs Kafkas Universitesi Veteriner Fakültesi Dergisi cryopreserved rumen liquor energy prediction fermentation gas production in vitro gas test volatile fatty acid |
| title | Utilization of cryopreserved ruminal liquor in in vitro gas production technique for evaluating nutritive value of some feedstuffs |
| title_full | Utilization of cryopreserved ruminal liquor in in vitro gas production technique for evaluating nutritive value of some feedstuffs |
| title_fullStr | Utilization of cryopreserved ruminal liquor in in vitro gas production technique for evaluating nutritive value of some feedstuffs |
| title_full_unstemmed | Utilization of cryopreserved ruminal liquor in in vitro gas production technique for evaluating nutritive value of some feedstuffs |
| title_short | Utilization of cryopreserved ruminal liquor in in vitro gas production technique for evaluating nutritive value of some feedstuffs |
| title_sort | utilization of cryopreserved ruminal liquor in in vitro gas production technique for evaluating nutritive value of some feedstuffs |
| topic | cryopreserved rumen liquor energy prediction fermentation gas production in vitro gas test volatile fatty acid |
| url | https://vetdergikafkas.org/pdf.php?id=2047 |
| work_keys_str_mv | AT gulsenn utilizationofcryopreservedruminalliquorininvitrogasproductiontechniqueforevaluatingnutritivevalueofsomefeedstuffs AT arikhd utilizationofcryopreservedruminalliquorininvitrogasproductiontechniqueforevaluatingnutritivevalueofsomefeedstuffs AT hayirlia utilizationofcryopreservedruminalliquorininvitrogasproductiontechniqueforevaluatingnutritivevalueofsomefeedstuffs AT alatasms utilizationofcryopreservedruminalliquorininvitrogasproductiontechniqueforevaluatingnutritivevalueofsomefeedstuffs AT aksoym utilizationofcryopreservedruminalliquorininvitrogasproductiontechniqueforevaluatingnutritivevalueofsomefeedstuffs |