oipA “on” status of Helicobacter pylori is associated with gastric cancer in North-Eastern Brazil
Abstract Background Although, outer membrane protein OipA of Helicobacter pylori has been associated with gastric mucosal damage and gastroduodenal diseases, studies evaluating gastric cancer patients are scarce. We investigated whether the functional oipA “on” status was associated with gastric can...
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| Format: | Article |
| Language: | English |
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BMC
2019-01-01
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| Series: | BMC Cancer |
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| Online Access: | http://link.springer.com/article/10.1186/s12885-018-5249-x |
| _version_ | 1818110169149603840 |
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| author | Lúcia Libanez Bessa Campelo Braga Maria Helane Rocha Batista Orleâncio Gomes Ripardo de Azevedo Kassiane Cristine da Silva Costa Adriana Dias Gomes Gifone Aguiar Rocha Dulciene Maria Magalhães Queiroz |
| author_facet | Lúcia Libanez Bessa Campelo Braga Maria Helane Rocha Batista Orleâncio Gomes Ripardo de Azevedo Kassiane Cristine da Silva Costa Adriana Dias Gomes Gifone Aguiar Rocha Dulciene Maria Magalhães Queiroz |
| author_sort | Lúcia Libanez Bessa Campelo Braga |
| collection | DOAJ |
| description | Abstract Background Although, outer membrane protein OipA of Helicobacter pylori has been associated with gastric mucosal damage and gastroduodenal diseases, studies evaluating gastric cancer patients are scarce. We investigated whether the functional oipA “on” status was associated with gastric cancer in the North-eastern Brazil, region with high prevalence of gastric cancer. Methods We included samples from 95 H. pylori positive subjects (23 patients with gastritis, 24 with gastric cancer, 32 first-degree relatives of gastric cancer patients and 16 children). oipA was assayed by polymerase chain reaction (PCR) and DNA sequencing. cagA and vacA status were evaluated by PCR. Results Overall 81.1% of the H. pylori strains had functional oipA. In adults, the oipA “on” status (OR = 9.20; 95%CI = 1.45–58.48, P = 0.02) and increasing age (OR = 1.08; 95%CI = 1.03–1.14; P = 0.003) were independently associated with gastric cancer in a logistic model. The oipA “on” status (OR = 14.75; 95%CI: 2.53–86.13, P = 0.003) was also associated with first-degree relatives of gastric cancer patients when compared with gastritis. The frequency of oipA “on” status did not differ between children and adults (P = 0.87). The oipA “on” status was significantly correlated with the presence of cagA and vacA s1 m1. Conclusion oipA “on” status was independently associated with gastric cancer and first-degree relatives of gastric cancer patients in North-eastern Brazil. |
| first_indexed | 2024-12-11T02:42:52Z |
| format | Article |
| id | doaj.art-a60c62ba14b04ee29c1113379438629d |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-12-11T02:42:52Z |
| publishDate | 2019-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-a60c62ba14b04ee29c1113379438629d2022-12-22T01:23:31ZengBMCBMC Cancer1471-24072019-01-011911710.1186/s12885-018-5249-xoipA “on” status of Helicobacter pylori is associated with gastric cancer in North-Eastern BrazilLúcia Libanez Bessa Campelo Braga0Maria Helane Rocha Batista1Orleâncio Gomes Ripardo de Azevedo2Kassiane Cristine da Silva Costa3Adriana Dias Gomes4Gifone Aguiar Rocha5Dulciene Maria Magalhães Queiroz6Clinical Research Unit, University Hospital Walter Cantídio/Department of Internal Medicine, Universidade Federal do CearáInstitute of Biomedicine, Universidade Federal do CearáInstitute of Biomedicine, Universidade Federal do CearáInstitute of Biomedicine, Universidade Federal do CearáLaboratory of Research in Bacteriology, Faculdade de Medicina, Universidade Federal de Minas GeraisLaboratory of Research in Bacteriology, Faculdade de Medicina, Universidade Federal de Minas GeraisLaboratory of Research in Bacteriology, Faculdade de Medicina, Universidade Federal de Minas GeraisAbstract Background Although, outer membrane protein OipA of Helicobacter pylori has been associated with gastric mucosal damage and gastroduodenal diseases, studies evaluating gastric cancer patients are scarce. We investigated whether the functional oipA “on” status was associated with gastric cancer in the North-eastern Brazil, region with high prevalence of gastric cancer. Methods We included samples from 95 H. pylori positive subjects (23 patients with gastritis, 24 with gastric cancer, 32 first-degree relatives of gastric cancer patients and 16 children). oipA was assayed by polymerase chain reaction (PCR) and DNA sequencing. cagA and vacA status were evaluated by PCR. Results Overall 81.1% of the H. pylori strains had functional oipA. In adults, the oipA “on” status (OR = 9.20; 95%CI = 1.45–58.48, P = 0.02) and increasing age (OR = 1.08; 95%CI = 1.03–1.14; P = 0.003) were independently associated with gastric cancer in a logistic model. The oipA “on” status (OR = 14.75; 95%CI: 2.53–86.13, P = 0.003) was also associated with first-degree relatives of gastric cancer patients when compared with gastritis. The frequency of oipA “on” status did not differ between children and adults (P = 0.87). The oipA “on” status was significantly correlated with the presence of cagA and vacA s1 m1. Conclusion oipA “on” status was independently associated with gastric cancer and first-degree relatives of gastric cancer patients in North-eastern Brazil.http://link.springer.com/article/10.1186/s12885-018-5249-xHelicobacter pylorioipAcagAvacAGastric cancer |
| spellingShingle | Lúcia Libanez Bessa Campelo Braga Maria Helane Rocha Batista Orleâncio Gomes Ripardo de Azevedo Kassiane Cristine da Silva Costa Adriana Dias Gomes Gifone Aguiar Rocha Dulciene Maria Magalhães Queiroz oipA “on” status of Helicobacter pylori is associated with gastric cancer in North-Eastern Brazil BMC Cancer Helicobacter pylori oipA cagA vacA Gastric cancer |
| title | oipA “on” status of Helicobacter pylori is associated with gastric cancer in North-Eastern Brazil |
| title_full | oipA “on” status of Helicobacter pylori is associated with gastric cancer in North-Eastern Brazil |
| title_fullStr | oipA “on” status of Helicobacter pylori is associated with gastric cancer in North-Eastern Brazil |
| title_full_unstemmed | oipA “on” status of Helicobacter pylori is associated with gastric cancer in North-Eastern Brazil |
| title_short | oipA “on” status of Helicobacter pylori is associated with gastric cancer in North-Eastern Brazil |
| title_sort | oipa on status of helicobacter pylori is associated with gastric cancer in north eastern brazil |
| topic | Helicobacter pylori oipA cagA vacA Gastric cancer |
| url | http://link.springer.com/article/10.1186/s12885-018-5249-x |
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