Long non-coding RNA TUG1 promotes proliferation and migration in PDGF-BB-stimulated HASMCs by regulating miR-216a-3p/SMURF2 axis
Abstract Background Abnormal proliferation and migration of human airway smooth muscle cells (HASMCs) play an important role in the development of childhood asthma. Long non-coding RNAs (lncRNAs) have been demonstrated to participate in HASMC proliferation and migration. We aimed to explore more eff...
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| Format: | Article |
| Language: | English |
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BMC
2021-11-01
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| Series: | BMC Molecular and Cell Biology |
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| Online Access: | https://doi.org/10.1186/s12860-021-00396-0 |
| _version_ | 1818823366393462784 |
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| author | Xinfang Wang Junsong Chen |
| author_facet | Xinfang Wang Junsong Chen |
| author_sort | Xinfang Wang |
| collection | DOAJ |
| description | Abstract Background Abnormal proliferation and migration of human airway smooth muscle cells (HASMCs) play an important role in the development of childhood asthma. Long non-coding RNAs (lncRNAs) have been demonstrated to participate in HASMC proliferation and migration. We aimed to explore more effects and molecular mechanism of taurine upregulated gene 1 (TUG1) in childhood asthma. Results TUG1 and SMURF2 were overexpressed and miR-216a-3p was downregulated in childhood asthma patients and PDGF-BB-stimulated HASMCs. TUG1 knockdown attenuated PDGF-BB-triggered proliferation and migration of HASMCs. MiR-216a-3p was targeted by TUG1, and miR-216a-3p suppression counteracted the repressive effects of TUG1 interference on proliferation and migration in PDGF-BB-treated HASMCs. SMURF2 was a downstream target of miR-216a-3p, and SMURF2 upregulation abated the inhibiting effects of miR-216a-3p on migration and proliferation in PDGF-BB-exposed HASMCs. TUG1 sponged miR-216a-3p to positively regulate SMURF2 expression. Conclusion TUG1 downregulation inhibited PDGF-BB-induced HASMC proliferation and migration by regulating miR-216a-3p/SMURF2 axis, offering novel insight into the potential application of TUG1 for childhood asthma treatment. |
| first_indexed | 2024-12-18T23:38:50Z |
| format | Article |
| id | doaj.art-a60d0e98ccd744a58f0a315e44dd5163 |
| institution | Directory Open Access Journal |
| issn | 2661-8850 |
| language | English |
| last_indexed | 2024-12-18T23:38:50Z |
| publishDate | 2021-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Molecular and Cell Biology |
| spelling | doaj.art-a60d0e98ccd744a58f0a315e44dd51632022-12-21T20:47:28ZengBMCBMC Molecular and Cell Biology2661-88502021-11-0122111110.1186/s12860-021-00396-0Long non-coding RNA TUG1 promotes proliferation and migration in PDGF-BB-stimulated HASMCs by regulating miR-216a-3p/SMURF2 axisXinfang Wang0Junsong Chen1Department of Pediatrics, Hangzhou First People’s Hospital Affiliated to Zhejiang UniversityRespiratory Department, Hangzhou Children’s HospitalAbstract Background Abnormal proliferation and migration of human airway smooth muscle cells (HASMCs) play an important role in the development of childhood asthma. Long non-coding RNAs (lncRNAs) have been demonstrated to participate in HASMC proliferation and migration. We aimed to explore more effects and molecular mechanism of taurine upregulated gene 1 (TUG1) in childhood asthma. Results TUG1 and SMURF2 were overexpressed and miR-216a-3p was downregulated in childhood asthma patients and PDGF-BB-stimulated HASMCs. TUG1 knockdown attenuated PDGF-BB-triggered proliferation and migration of HASMCs. MiR-216a-3p was targeted by TUG1, and miR-216a-3p suppression counteracted the repressive effects of TUG1 interference on proliferation and migration in PDGF-BB-treated HASMCs. SMURF2 was a downstream target of miR-216a-3p, and SMURF2 upregulation abated the inhibiting effects of miR-216a-3p on migration and proliferation in PDGF-BB-exposed HASMCs. TUG1 sponged miR-216a-3p to positively regulate SMURF2 expression. Conclusion TUG1 downregulation inhibited PDGF-BB-induced HASMC proliferation and migration by regulating miR-216a-3p/SMURF2 axis, offering novel insight into the potential application of TUG1 for childhood asthma treatment.https://doi.org/10.1186/s12860-021-00396-0Childhood asthmaTUG1miR-216a-3pSMURF2HASMCs |
| spellingShingle | Xinfang Wang Junsong Chen Long non-coding RNA TUG1 promotes proliferation and migration in PDGF-BB-stimulated HASMCs by regulating miR-216a-3p/SMURF2 axis BMC Molecular and Cell Biology Childhood asthma TUG1 miR-216a-3p SMURF2 HASMCs |
| title | Long non-coding RNA TUG1 promotes proliferation and migration in PDGF-BB-stimulated HASMCs by regulating miR-216a-3p/SMURF2 axis |
| title_full | Long non-coding RNA TUG1 promotes proliferation and migration in PDGF-BB-stimulated HASMCs by regulating miR-216a-3p/SMURF2 axis |
| title_fullStr | Long non-coding RNA TUG1 promotes proliferation and migration in PDGF-BB-stimulated HASMCs by regulating miR-216a-3p/SMURF2 axis |
| title_full_unstemmed | Long non-coding RNA TUG1 promotes proliferation and migration in PDGF-BB-stimulated HASMCs by regulating miR-216a-3p/SMURF2 axis |
| title_short | Long non-coding RNA TUG1 promotes proliferation and migration in PDGF-BB-stimulated HASMCs by regulating miR-216a-3p/SMURF2 axis |
| title_sort | long non coding rna tug1 promotes proliferation and migration in pdgf bb stimulated hasmcs by regulating mir 216a 3p smurf2 axis |
| topic | Childhood asthma TUG1 miR-216a-3p SMURF2 HASMCs |
| url | https://doi.org/10.1186/s12860-021-00396-0 |
| work_keys_str_mv | AT xinfangwang longnoncodingrnatug1promotesproliferationandmigrationinpdgfbbstimulatedhasmcsbyregulatingmir216a3psmurf2axis AT junsongchen longnoncodingrnatug1promotesproliferationandmigrationinpdgfbbstimulatedhasmcsbyregulatingmir216a3psmurf2axis |