A single Proteus mirabilis lineage from human and animal sources: a hidden reservoir of OXA-23 or OXA-58 carbapenemases in Enterobacterales
Abstract In Enterobacterales, the most common carbapenemases are Ambler’s class A (KPC-like), class B (NDM-, VIM- or IMP-like) or class D (OXA-48-like) enzymes. This study describes the characterization of twenty-four OXA-23 or OXA-58 producing-Proteus mirabilis isolates recovered from human and vet...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
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Nature Portfolio
2020-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-020-66161-z |
| _version_ | 1818402949638914048 |
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| author | Rémy A. Bonnin Delphine Girlich Agnès B. Jousset Lauraine Gauthier Gaëlle Cuzon Pierre Bogaerts Marisa Haenni Jean-Yves Madec Elodie Couvé-Deacon Olivier Barraud Nicolas Fortineau Philippe Glaser Youri Glupczynski Laurent Dortet Thierry Naas |
| author_facet | Rémy A. Bonnin Delphine Girlich Agnès B. Jousset Lauraine Gauthier Gaëlle Cuzon Pierre Bogaerts Marisa Haenni Jean-Yves Madec Elodie Couvé-Deacon Olivier Barraud Nicolas Fortineau Philippe Glaser Youri Glupczynski Laurent Dortet Thierry Naas |
| author_sort | Rémy A. Bonnin |
| collection | DOAJ |
| description | Abstract In Enterobacterales, the most common carbapenemases are Ambler’s class A (KPC-like), class B (NDM-, VIM- or IMP-like) or class D (OXA-48-like) enzymes. This study describes the characterization of twenty-four OXA-23 or OXA-58 producing-Proteus mirabilis isolates recovered from human and veterinary samples from France and Belgium. Twenty-two P. mirabilis isolates producing either OXA-23 (n = 21) or OXA-58 (n = 1), collected between 2013 and 2018, as well as 2 reference strains isolated in 1996 and 2015 were fully sequenced. Phylogenetic analysis revealed that 22 of the 24 isolates, including the isolate from 1996, belonged to a single lineage that has disseminated in humans and animals over a long period of time. The bla OXA-23 gene was located on the chromosome and was part of a composite transposon, Tn6703, bracketed by two copies of IS15∆II. Sequencing using Pacbio long read technology of OXA-23-producing P. mirabilis VAC allowed the assembly of a 55.5-kb structure encompassing the bla OXA-23 gene in that isolate. By contrast to the bla OXA-23 genes, the bla OXA-58 gene of P. mirabilis CNR20130297 was identified on a 6-kb plasmid. The acquisition of the bla OXA-58 gene on this plasmid involved XerC-XerD recombinases. Our results suggest that a major clone of OXA-23-producing P. mirabilis is circulating in France and Belgium since 1996. |
| first_indexed | 2024-12-14T08:16:29Z |
| format | Article |
| id | doaj.art-a61067770b2c4ff9b0017099f6999791 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-14T08:16:29Z |
| publishDate | 2020-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-a61067770b2c4ff9b0017099f69997912022-12-21T23:09:55ZengNature PortfolioScientific Reports2045-23222020-06-011011910.1038/s41598-020-66161-zA single Proteus mirabilis lineage from human and animal sources: a hidden reservoir of OXA-23 or OXA-58 carbapenemases in EnterobacteralesRémy A. Bonnin0Delphine Girlich1Agnès B. Jousset2Lauraine Gauthier3Gaëlle Cuzon4Pierre Bogaerts5Marisa Haenni6Jean-Yves Madec7Elodie Couvé-Deacon8Olivier Barraud9Nicolas Fortineau10Philippe Glaser11Youri Glupczynski12Laurent Dortet13Thierry Naas14UMR 1184, Team Resist, INSERM, Paris-Saclay University, Faculty of MedicineUMR 1184, Team Resist, INSERM, Paris-Saclay University, Faculty of MedicineUMR 1184, Team Resist, INSERM, Paris-Saclay University, Faculty of MedicineUMR 1184, Team Resist, INSERM, Paris-Saclay University, Faculty of MedicineUMR 1184, Team Resist, INSERM, Paris-Saclay University, Faculty of MedicineBelgian National Reference Laboratory for Monitoring of Antimicrobial Resistance in Gram-Negative Bacteria, CHU UCL NamurUnité Antibiorésistance et Virulence Bactériennes, Université de Lyon - ANSES Laboratoire de LyonUnité Antibiorésistance et Virulence Bactériennes, Université de Lyon - ANSES Laboratoire de LyonUniversité de Limoges, INSERM, CHU LimogesUniversité de Limoges, INSERM, CHU LimogesUMR 1184, Team Resist, INSERM, Paris-Saclay University, Faculty of MedicineJoint research Unit EERA « Evolution and Ecology of Resistance to Antibiotics », Institut Pasteur-APHP-University Paris SudBelgian National Reference Laboratory for Monitoring of Antimicrobial Resistance in Gram-Negative Bacteria, CHU UCL NamurUMR 1184, Team Resist, INSERM, Paris-Saclay University, Faculty of MedicineUMR 1184, Team Resist, INSERM, Paris-Saclay University, Faculty of MedicineAbstract In Enterobacterales, the most common carbapenemases are Ambler’s class A (KPC-like), class B (NDM-, VIM- or IMP-like) or class D (OXA-48-like) enzymes. This study describes the characterization of twenty-four OXA-23 or OXA-58 producing-Proteus mirabilis isolates recovered from human and veterinary samples from France and Belgium. Twenty-two P. mirabilis isolates producing either OXA-23 (n = 21) or OXA-58 (n = 1), collected between 2013 and 2018, as well as 2 reference strains isolated in 1996 and 2015 were fully sequenced. Phylogenetic analysis revealed that 22 of the 24 isolates, including the isolate from 1996, belonged to a single lineage that has disseminated in humans and animals over a long period of time. The bla OXA-23 gene was located on the chromosome and was part of a composite transposon, Tn6703, bracketed by two copies of IS15∆II. Sequencing using Pacbio long read technology of OXA-23-producing P. mirabilis VAC allowed the assembly of a 55.5-kb structure encompassing the bla OXA-23 gene in that isolate. By contrast to the bla OXA-23 genes, the bla OXA-58 gene of P. mirabilis CNR20130297 was identified on a 6-kb plasmid. The acquisition of the bla OXA-58 gene on this plasmid involved XerC-XerD recombinases. Our results suggest that a major clone of OXA-23-producing P. mirabilis is circulating in France and Belgium since 1996.https://doi.org/10.1038/s41598-020-66161-z |
| spellingShingle | Rémy A. Bonnin Delphine Girlich Agnès B. Jousset Lauraine Gauthier Gaëlle Cuzon Pierre Bogaerts Marisa Haenni Jean-Yves Madec Elodie Couvé-Deacon Olivier Barraud Nicolas Fortineau Philippe Glaser Youri Glupczynski Laurent Dortet Thierry Naas A single Proteus mirabilis lineage from human and animal sources: a hidden reservoir of OXA-23 or OXA-58 carbapenemases in Enterobacterales Scientific Reports |
| title | A single Proteus mirabilis lineage from human and animal sources: a hidden reservoir of OXA-23 or OXA-58 carbapenemases in Enterobacterales |
| title_full | A single Proteus mirabilis lineage from human and animal sources: a hidden reservoir of OXA-23 or OXA-58 carbapenemases in Enterobacterales |
| title_fullStr | A single Proteus mirabilis lineage from human and animal sources: a hidden reservoir of OXA-23 or OXA-58 carbapenemases in Enterobacterales |
| title_full_unstemmed | A single Proteus mirabilis lineage from human and animal sources: a hidden reservoir of OXA-23 or OXA-58 carbapenemases in Enterobacterales |
| title_short | A single Proteus mirabilis lineage from human and animal sources: a hidden reservoir of OXA-23 or OXA-58 carbapenemases in Enterobacterales |
| title_sort | single proteus mirabilis lineage from human and animal sources a hidden reservoir of oxa 23 or oxa 58 carbapenemases in enterobacterales |
| url | https://doi.org/10.1038/s41598-020-66161-z |
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