Cloning an RBD-T4-LINKER-C5a sequence encoding the SARS-CoV-2 antigen into a plant expression vector

This study aims to make a plant expression vector with an RBD-T4-Linker-C5a sequence that codes for the SARS-CoV-2 antigen and an A3Dsp signal peptide from the rice 3D amylase gene located before the RBD. Methods of molecular cloning were applied in this study. The plant expression vector pNHL22 ha...

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Main Authors: Nguyen Hoang Tue, Nguyen Ngoc Luong, Nguyen Quang Duc Tien
Format: Article
Language:English
Published: HUJOS 2023-12-01
Series:Tạp chí Khoa học Đại học Huế: Khoa học Tự nhiên
Subjects:
Online Access:https://jos.hueuni.edu.vn/index.php/hujos-ns/article/view/7075
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author Nguyen Hoang Tue
Nguyen Ngoc Luong
Nguyen Quang Duc Tien
author_facet Nguyen Hoang Tue
Nguyen Ngoc Luong
Nguyen Quang Duc Tien
author_sort Nguyen Hoang Tue
collection DOAJ
description This study aims to make a plant expression vector with an RBD-T4-Linker-C5a sequence that codes for the SARS-CoV-2 antigen and an A3Dsp signal peptide from the rice 3D amylase gene located before the RBD. Methods of molecular cloning were applied in this study. The plant expression vector pNHL22 harboring the RBD-T4-Linker-C5a sequence was successfully established and conjugated into Agrobacterium tumefaciens LBA4404 by triparental mating. Bacteria A. tumefaciens containing the RBD-T4-Linker-C5a sequence are now ready for genetic transformation into the Nicotiana benthamiana plant for future applications.
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spelling doaj.art-a611bd76fb304950b46574db3cc0b4f02024-02-28T09:34:04ZengHUJOSTạp chí Khoa học Đại học Huế: Khoa học Tự nhiên1859-13882615-96782023-12-011321D10.26459/hueunijns.v132i1D.7075Cloning an RBD-T4-LINKER-C5a sequence encoding the SARS-CoV-2 antigen into a plant expression vectorNguyen Hoang Tue0Nguyen Ngoc Luong1Nguyen Quang Duc Tien2Institute of Bioactive Compounds (IBT), Hue University of Sciences, Hue University, 77 Nguyen Hue, Hue, Viet NamInstitute of Bioactive Compounds (IBT), Hue University of Sciences, Hue University, 77 Nguyen Hue, Hue, Viet NamInstitute of Bioactive Compounds (IBT), Hue University of Sciences, Hue University, 77 Nguyen Hue, Hue, Viet Nam This study aims to make a plant expression vector with an RBD-T4-Linker-C5a sequence that codes for the SARS-CoV-2 antigen and an A3Dsp signal peptide from the rice 3D amylase gene located before the RBD. Methods of molecular cloning were applied in this study. The plant expression vector pNHL22 harboring the RBD-T4-Linker-C5a sequence was successfully established and conjugated into Agrobacterium tumefaciens LBA4404 by triparental mating. Bacteria A. tumefaciens containing the RBD-T4-Linker-C5a sequence are now ready for genetic transformation into the Nicotiana benthamiana plant for future applications. https://jos.hueuni.edu.vn/index.php/hujos-ns/article/view/7075SARS-CoV-2 antigenRBDC5AT4Cloning
spellingShingle Nguyen Hoang Tue
Nguyen Ngoc Luong
Nguyen Quang Duc Tien
Cloning an RBD-T4-LINKER-C5a sequence encoding the SARS-CoV-2 antigen into a plant expression vector
Tạp chí Khoa học Đại học Huế: Khoa học Tự nhiên
SARS-CoV-2 antigen
RBD
C5A
T4
Cloning
title Cloning an RBD-T4-LINKER-C5a sequence encoding the SARS-CoV-2 antigen into a plant expression vector
title_full Cloning an RBD-T4-LINKER-C5a sequence encoding the SARS-CoV-2 antigen into a plant expression vector
title_fullStr Cloning an RBD-T4-LINKER-C5a sequence encoding the SARS-CoV-2 antigen into a plant expression vector
title_full_unstemmed Cloning an RBD-T4-LINKER-C5a sequence encoding the SARS-CoV-2 antigen into a plant expression vector
title_short Cloning an RBD-T4-LINKER-C5a sequence encoding the SARS-CoV-2 antigen into a plant expression vector
title_sort cloning an rbd t4 linker c5a sequence encoding the sars cov 2 antigen into a plant expression vector
topic SARS-CoV-2 antigen
RBD
C5A
T4
Cloning
url https://jos.hueuni.edu.vn/index.php/hujos-ns/article/view/7075
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AT nguyenquangductien cloninganrbdt4linkerc5asequenceencodingthesarscov2antigenintoaplantexpressionvector