Cloning an RBD-T4-LINKER-C5a sequence encoding the SARS-CoV-2 antigen into a plant expression vector
This study aims to make a plant expression vector with an RBD-T4-Linker-C5a sequence that codes for the SARS-CoV-2 antigen and an A3Dsp signal peptide from the rice 3D amylase gene located before the RBD. Methods of molecular cloning were applied in this study. The plant expression vector pNHL22 ha...
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Format: | Article |
Language: | English |
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HUJOS
2023-12-01
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Series: | Tạp chí Khoa học Đại học Huế: Khoa học Tự nhiên |
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Online Access: | https://jos.hueuni.edu.vn/index.php/hujos-ns/article/view/7075 |
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author | Nguyen Hoang Tue Nguyen Ngoc Luong Nguyen Quang Duc Tien |
author_facet | Nguyen Hoang Tue Nguyen Ngoc Luong Nguyen Quang Duc Tien |
author_sort | Nguyen Hoang Tue |
collection | DOAJ |
description |
This study aims to make a plant expression vector with an RBD-T4-Linker-C5a sequence that codes for the SARS-CoV-2 antigen and an A3Dsp signal peptide from the rice 3D amylase gene located before the RBD. Methods of molecular cloning were applied in this study. The plant expression vector pNHL22 harboring the RBD-T4-Linker-C5a sequence was successfully established and conjugated into Agrobacterium tumefaciens LBA4404 by triparental mating. Bacteria A. tumefaciens containing the RBD-T4-Linker-C5a sequence are now ready for genetic transformation into the Nicotiana benthamiana plant for future applications.
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first_indexed | 2024-03-07T20:00:20Z |
format | Article |
id | doaj.art-a611bd76fb304950b46574db3cc0b4f0 |
institution | Directory Open Access Journal |
issn | 1859-1388 2615-9678 |
language | English |
last_indexed | 2024-03-07T20:00:20Z |
publishDate | 2023-12-01 |
publisher | HUJOS |
record_format | Article |
series | Tạp chí Khoa học Đại học Huế: Khoa học Tự nhiên |
spelling | doaj.art-a611bd76fb304950b46574db3cc0b4f02024-02-28T09:34:04ZengHUJOSTạp chí Khoa học Đại học Huế: Khoa học Tự nhiên1859-13882615-96782023-12-011321D10.26459/hueunijns.v132i1D.7075Cloning an RBD-T4-LINKER-C5a sequence encoding the SARS-CoV-2 antigen into a plant expression vectorNguyen Hoang Tue0Nguyen Ngoc Luong1Nguyen Quang Duc Tien2Institute of Bioactive Compounds (IBT), Hue University of Sciences, Hue University, 77 Nguyen Hue, Hue, Viet NamInstitute of Bioactive Compounds (IBT), Hue University of Sciences, Hue University, 77 Nguyen Hue, Hue, Viet NamInstitute of Bioactive Compounds (IBT), Hue University of Sciences, Hue University, 77 Nguyen Hue, Hue, Viet Nam This study aims to make a plant expression vector with an RBD-T4-Linker-C5a sequence that codes for the SARS-CoV-2 antigen and an A3Dsp signal peptide from the rice 3D amylase gene located before the RBD. Methods of molecular cloning were applied in this study. The plant expression vector pNHL22 harboring the RBD-T4-Linker-C5a sequence was successfully established and conjugated into Agrobacterium tumefaciens LBA4404 by triparental mating. Bacteria A. tumefaciens containing the RBD-T4-Linker-C5a sequence are now ready for genetic transformation into the Nicotiana benthamiana plant for future applications. https://jos.hueuni.edu.vn/index.php/hujos-ns/article/view/7075SARS-CoV-2 antigenRBDC5AT4Cloning |
spellingShingle | Nguyen Hoang Tue Nguyen Ngoc Luong Nguyen Quang Duc Tien Cloning an RBD-T4-LINKER-C5a sequence encoding the SARS-CoV-2 antigen into a plant expression vector Tạp chí Khoa học Đại học Huế: Khoa học Tự nhiên SARS-CoV-2 antigen RBD C5A T4 Cloning |
title | Cloning an RBD-T4-LINKER-C5a sequence encoding the SARS-CoV-2 antigen into a plant expression vector |
title_full | Cloning an RBD-T4-LINKER-C5a sequence encoding the SARS-CoV-2 antigen into a plant expression vector |
title_fullStr | Cloning an RBD-T4-LINKER-C5a sequence encoding the SARS-CoV-2 antigen into a plant expression vector |
title_full_unstemmed | Cloning an RBD-T4-LINKER-C5a sequence encoding the SARS-CoV-2 antigen into a plant expression vector |
title_short | Cloning an RBD-T4-LINKER-C5a sequence encoding the SARS-CoV-2 antigen into a plant expression vector |
title_sort | cloning an rbd t4 linker c5a sequence encoding the sars cov 2 antigen into a plant expression vector |
topic | SARS-CoV-2 antigen RBD C5A T4 Cloning |
url | https://jos.hueuni.edu.vn/index.php/hujos-ns/article/view/7075 |
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