Genome sequence of multidrug-resistant Erysipelothrix rhusiopathiae ZJ carrying several acquired antimicrobial resistance genes
Objectives: This study aimed to determine the genetic environment of antimicrobial resistance genes (ARGs) in Erysipelothrix rhusiopathiae strain ZJ isolated from a pig with symptoms of swine erysipelas in China. Methods: Illumina MiSeq (200× coverage) and PacBio RS II (100× coverage) platforms were...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-06-01
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| Series: | Journal of Global Antimicrobial Resistance |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716520300461 |
| _version_ | 1819046146242248704 |
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| author | Ju Gu Yun-xia Li Chang-wen Xu Xian-jun Xie Ping Li Guang-xu Ma Chang-wei Lei Jin-xin Liu An-yun Zhang |
| author_facet | Ju Gu Yun-xia Li Chang-wen Xu Xian-jun Xie Ping Li Guang-xu Ma Chang-wei Lei Jin-xin Liu An-yun Zhang |
| author_sort | Ju Gu |
| collection | DOAJ |
| description | Objectives: This study aimed to determine the genetic environment of antimicrobial resistance genes (ARGs) in Erysipelothrix rhusiopathiae strain ZJ isolated from a pig with symptoms of swine erysipelas in China. Methods: Illumina MiSeq (200× coverage) and PacBio RS II (100× coverage) platforms were used for genome sequencing. ARGs and prophages were identified using ResFinder 3.0 and PHASTER, respectively. A conjugation experiment, induced prophage infection and long-term passage assay were performed to determine the transferability and stability of ARGs in this strain. Results: The assembled circular genome of E. rhusiopathiae ZJ was 1 945 689 bp with a GC content of 36.48%; no plasmid sequence was detected. Eleven acquired ARGs were identified in the genome. A novel integrative and conjugative element (ICE) encoding a multidrug resistance (MDR) gene cluster [aadE–apt–spw–lsa(E)–lnu(B)–aadE–sat4–aphA3] was identified in strain ZJ. A prophage Φ1605 harbouring mef(A)–msr(D) and tet(M) was also found in this strain, which can take a circular form and can be induced by mitomycin C to infect E. rhusiopathiae G4T10 for ARG transfer. Conclusion: To our knowledge, this is the first report of a complete genome sequence of E. rhusiopathiae carrying multiple ARGs obtained from a pig farm. This is the first identification of a novel chimeric ICE carrying a MDR gene cluster and a prophage carrying ARGs in E. rhusiopathiae, which will provide a valuable reference to understand the potential transfer mechanism of MDR gene clusters carried by ICEs and prophages in Gram-positive bacteria. |
| first_indexed | 2024-12-21T10:39:50Z |
| format | Article |
| id | doaj.art-a6157b55c9014747bfb3a6f879cb12ee |
| institution | Directory Open Access Journal |
| issn | 2213-7165 |
| language | English |
| last_indexed | 2024-12-21T10:39:50Z |
| publishDate | 2020-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj.art-a6157b55c9014747bfb3a6f879cb12ee2022-12-21T19:06:58ZengElsevierJournal of Global Antimicrobial Resistance2213-71652020-06-01211315Genome sequence of multidrug-resistant Erysipelothrix rhusiopathiae ZJ carrying several acquired antimicrobial resistance genesJu Gu0Yun-xia Li1Chang-wen Xu2Xian-jun Xie3Ping Li4Guang-xu Ma5Chang-wei Lei6Jin-xin Liu7An-yun Zhang8Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Sciences, Sichuan University, and Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Chengdu, Sichuan, PR ChinaKey Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Sciences, Sichuan University, and Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Chengdu, Sichuan, PR ChinaKey Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Sciences, Sichuan University, and Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Chengdu, Sichuan, PR China; Sichuan Academy of Grassland Sciences, Chengdu, Sichuan, PR ChinaKey Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Sciences, Sichuan University, and Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Chengdu, Sichuan, PR ChinaKey Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Sciences, Sichuan University, and Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Chengdu, Sichuan, PR ChinaDepartment of Veterinary Biosciences, Melbourne Veterinary School, The University of Melbourne, Parkville, VIC 3010, AustraliaKey Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Sciences, Sichuan University, and Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Chengdu, Sichuan, PR ChinaDepartment of Food Science and Technology, Robert Mondavi Institute for Wine and Food Science, University of California, Davis, CA 95616, USAKey Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Sciences, Sichuan University, and Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Chengdu, Sichuan, PR China; Corresponding author. Present address: College of Life Sciences, Sichuan University, No. 29 Wangjiang Road, Chengdu, Sichuan 610064, PR China.Objectives: This study aimed to determine the genetic environment of antimicrobial resistance genes (ARGs) in Erysipelothrix rhusiopathiae strain ZJ isolated from a pig with symptoms of swine erysipelas in China. Methods: Illumina MiSeq (200× coverage) and PacBio RS II (100× coverage) platforms were used for genome sequencing. ARGs and prophages were identified using ResFinder 3.0 and PHASTER, respectively. A conjugation experiment, induced prophage infection and long-term passage assay were performed to determine the transferability and stability of ARGs in this strain. Results: The assembled circular genome of E. rhusiopathiae ZJ was 1 945 689 bp with a GC content of 36.48%; no plasmid sequence was detected. Eleven acquired ARGs were identified in the genome. A novel integrative and conjugative element (ICE) encoding a multidrug resistance (MDR) gene cluster [aadE–apt–spw–lsa(E)–lnu(B)–aadE–sat4–aphA3] was identified in strain ZJ. A prophage Φ1605 harbouring mef(A)–msr(D) and tet(M) was also found in this strain, which can take a circular form and can be induced by mitomycin C to infect E. rhusiopathiae G4T10 for ARG transfer. Conclusion: To our knowledge, this is the first report of a complete genome sequence of E. rhusiopathiae carrying multiple ARGs obtained from a pig farm. This is the first identification of a novel chimeric ICE carrying a MDR gene cluster and a prophage carrying ARGs in E. rhusiopathiae, which will provide a valuable reference to understand the potential transfer mechanism of MDR gene clusters carried by ICEs and prophages in Gram-positive bacteria.http://www.sciencedirect.com/science/article/pii/S2213716520300461Erysipelothrix rhusiopathiaeMultidrug resistanceIntegrative and conjugative elementProphage |
| spellingShingle | Ju Gu Yun-xia Li Chang-wen Xu Xian-jun Xie Ping Li Guang-xu Ma Chang-wei Lei Jin-xin Liu An-yun Zhang Genome sequence of multidrug-resistant Erysipelothrix rhusiopathiae ZJ carrying several acquired antimicrobial resistance genes Journal of Global Antimicrobial Resistance Erysipelothrix rhusiopathiae Multidrug resistance Integrative and conjugative element Prophage |
| title | Genome sequence of multidrug-resistant Erysipelothrix rhusiopathiae ZJ carrying several acquired antimicrobial resistance genes |
| title_full | Genome sequence of multidrug-resistant Erysipelothrix rhusiopathiae ZJ carrying several acquired antimicrobial resistance genes |
| title_fullStr | Genome sequence of multidrug-resistant Erysipelothrix rhusiopathiae ZJ carrying several acquired antimicrobial resistance genes |
| title_full_unstemmed | Genome sequence of multidrug-resistant Erysipelothrix rhusiopathiae ZJ carrying several acquired antimicrobial resistance genes |
| title_short | Genome sequence of multidrug-resistant Erysipelothrix rhusiopathiae ZJ carrying several acquired antimicrobial resistance genes |
| title_sort | genome sequence of multidrug resistant erysipelothrix rhusiopathiae zj carrying several acquired antimicrobial resistance genes |
| topic | Erysipelothrix rhusiopathiae Multidrug resistance Integrative and conjugative element Prophage |
| url | http://www.sciencedirect.com/science/article/pii/S2213716520300461 |
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