Effect of BMP-2 Adherent to Resorbable Sutures on Cartilage Repair: A Rat Model of Xyphoid Process
Meniscal tears are often seen in orthopedic practice. The current strategy for meniscal repair has only had limited success with a relatively high incidence of re-operative rate. This study evaluates the therapeutic effects of Bone morphogenetic protein-2 (BMP-2) soaked sutures for cartilage repair,...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-08-01
|
| Series: | Materials |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1996-1944/13/17/3764 |
| _version_ | 1797555543615733760 |
|---|---|
| author | Nathan Drummond Bradley W. Bruner Michael H. Heggeness Bradley Dart Shang-You Yang |
| author_facet | Nathan Drummond Bradley W. Bruner Michael H. Heggeness Bradley Dart Shang-You Yang |
| author_sort | Nathan Drummond |
| collection | DOAJ |
| description | Meniscal tears are often seen in orthopedic practice. The current strategy for meniscal repair has only had limited success with a relatively high incidence of re-operative rate. This study evaluates the therapeutic effects of Bone morphogenetic protein-2 (BMP-2) soaked sutures for cartilage repair, using a rat model of xyphoid healing. Vicryl-resorbable sutures were presoaked in BMP-2 solutions prior to animal experimentation. Rat xyphoid process (an avascular hyaline cartilage structure) was surgically ruptured followed by repair procedures with regular suture or with sutures that were pre-soaked in BMP-2 solutions. In vitro assessment indicated that presoaking the Vicryl-resorbable sutures with 10 µg/mL BMP-2 resulted in a sustained amount of the growth factor release up to 7 days. Histological analysis suggested that application of this BMP-2 soaked suture on the rat xyphoid process model significantly improved the avascular cartilage healing compared to non-soaked control sutures. In conclusion, data here confirm that the rat xyphoid process repair is a reproducible and inexpensive animal model for meniscus and other cartilage repair. More importantly, coating of BMP-2 on sutures appears a potential avenue to improve cartilage repair and regeneration. Further study is warranted to explore the molecular mechanisms of this strategy. |
| first_indexed | 2024-03-10T16:49:01Z |
| format | Article |
| id | doaj.art-a6162eaf647742e78199a94f34f4e977 |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-10T16:49:01Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-a6162eaf647742e78199a94f34f4e9772023-11-20T11:23:11ZengMDPI AGMaterials1996-19442020-08-011317376410.3390/ma13173764Effect of BMP-2 Adherent to Resorbable Sutures on Cartilage Repair: A Rat Model of Xyphoid ProcessNathan Drummond0Bradley W. Bruner1Michael H. Heggeness2Bradley Dart3Shang-You Yang4Department of Orthopaedic Surgery, University of Kansas School of Medicine-Wichita, Wichita, KS 67214, USADepartment of Orthopaedic Surgery, University of Kansas School of Medicine-Wichita, Wichita, KS 67214, USADepartment of Orthopaedic Surgery, University of Kansas School of Medicine-Wichita, Wichita, KS 67214, USADepartment of Orthopaedic Surgery, University of Kansas School of Medicine-Wichita, Wichita, KS 67214, USADepartment of Orthopaedic Surgery, University of Kansas School of Medicine-Wichita, Wichita, KS 67214, USAMeniscal tears are often seen in orthopedic practice. The current strategy for meniscal repair has only had limited success with a relatively high incidence of re-operative rate. This study evaluates the therapeutic effects of Bone morphogenetic protein-2 (BMP-2) soaked sutures for cartilage repair, using a rat model of xyphoid healing. Vicryl-resorbable sutures were presoaked in BMP-2 solutions prior to animal experimentation. Rat xyphoid process (an avascular hyaline cartilage structure) was surgically ruptured followed by repair procedures with regular suture or with sutures that were pre-soaked in BMP-2 solutions. In vitro assessment indicated that presoaking the Vicryl-resorbable sutures with 10 µg/mL BMP-2 resulted in a sustained amount of the growth factor release up to 7 days. Histological analysis suggested that application of this BMP-2 soaked suture on the rat xyphoid process model significantly improved the avascular cartilage healing compared to non-soaked control sutures. In conclusion, data here confirm that the rat xyphoid process repair is a reproducible and inexpensive animal model for meniscus and other cartilage repair. More importantly, coating of BMP-2 on sutures appears a potential avenue to improve cartilage repair and regeneration. Further study is warranted to explore the molecular mechanisms of this strategy.https://www.mdpi.com/1996-1944/13/17/3764resorbable sutureBMP-2meniscal repairxyphoid processanimal model |
| spellingShingle | Nathan Drummond Bradley W. Bruner Michael H. Heggeness Bradley Dart Shang-You Yang Effect of BMP-2 Adherent to Resorbable Sutures on Cartilage Repair: A Rat Model of Xyphoid Process Materials resorbable suture BMP-2 meniscal repair xyphoid process animal model |
| title | Effect of BMP-2 Adherent to Resorbable Sutures on Cartilage Repair: A Rat Model of Xyphoid Process |
| title_full | Effect of BMP-2 Adherent to Resorbable Sutures on Cartilage Repair: A Rat Model of Xyphoid Process |
| title_fullStr | Effect of BMP-2 Adherent to Resorbable Sutures on Cartilage Repair: A Rat Model of Xyphoid Process |
| title_full_unstemmed | Effect of BMP-2 Adherent to Resorbable Sutures on Cartilage Repair: A Rat Model of Xyphoid Process |
| title_short | Effect of BMP-2 Adherent to Resorbable Sutures on Cartilage Repair: A Rat Model of Xyphoid Process |
| title_sort | effect of bmp 2 adherent to resorbable sutures on cartilage repair a rat model of xyphoid process |
| topic | resorbable suture BMP-2 meniscal repair xyphoid process animal model |
| url | https://www.mdpi.com/1996-1944/13/17/3764 |
| work_keys_str_mv | AT nathandrummond effectofbmp2adherenttoresorbablesuturesoncartilagerepairaratmodelofxyphoidprocess AT bradleywbruner effectofbmp2adherenttoresorbablesuturesoncartilagerepairaratmodelofxyphoidprocess AT michaelhheggeness effectofbmp2adherenttoresorbablesuturesoncartilagerepairaratmodelofxyphoidprocess AT bradleydart effectofbmp2adherenttoresorbablesuturesoncartilagerepairaratmodelofxyphoidprocess AT shangyouyang effectofbmp2adherenttoresorbablesuturesoncartilagerepairaratmodelofxyphoidprocess |