Argininyl-fructosyl-glucose (AFG) antagonizes D-galactose-induced cellular senescence of neuro-2a via inhibiting endoplasmic reticulum stress and apoptosis
Argininyl-fructosyl-glucose (AFG), a distinctive non-saponin compound isolated from red ginseng (Panax ginseng. C.A. Meyer), demonstrates neuroprotective effects. However, the molecular mechanism underlying AFG's anti-aging properties remains unclear. This study established an in vitro D-galact...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-04-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S175646462400104X |
| _version_ | 1797256432424321024 |
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| author | Shan Tang Yun-yi Hou Rui-yi Hu Jun-jie Zhang Xu-fei Gao Ming Zhang Zi Wang Rui Zhang Pin-hui Jia Wei Li |
| author_facet | Shan Tang Yun-yi Hou Rui-yi Hu Jun-jie Zhang Xu-fei Gao Ming Zhang Zi Wang Rui Zhang Pin-hui Jia Wei Li |
| author_sort | Shan Tang |
| collection | DOAJ |
| description | Argininyl-fructosyl-glucose (AFG), a distinctive non-saponin compound isolated from red ginseng (Panax ginseng. C.A. Meyer), demonstrates neuroprotective effects. However, the molecular mechanism underlying AFG's anti-aging properties remains unclear. This study established an in vitro D-galactose-induced N2a cells subacute aging model to investigate AFG's anti-aging activity and potential mechanisms. Results indicated that AFG treatment (8 μM) significantly reduces the overexpression of aging-related proteins (p53/p21/p16) induced by D-galactose, highlighting its anti-aging efficacy. AFG concurrently diminishes ROS accumulation, lowering extracellular lactate dehydrogenase and alleviating intracellular oxidative stress. Notably, AFG suppresses the PERK/CHOP/caspase 12 pathway, reducing intracellular Ca2+ concentration and mitigating endoplasmic reticulum (ER) stress. Additionally, AFG decreases Bax and caspase 3 expression, elevates Bcl-2 and mitochondrial membrane potential, mitigating apoptosis. NAC with 4-PBA validated the results. Overall, AFG protects N2a cells from aging by reducing ER stress and apoptosis, suggesting its potential as a key substance for treating aging and neurodegenerative diseases. |
| first_indexed | 2024-03-07T14:30:18Z |
| format | Article |
| id | doaj.art-a6197ebd18174aae82bf598a88ed88b9 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-04-24T22:21:39Z |
| publishDate | 2024-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-a6197ebd18174aae82bf598a88ed88b92024-03-20T06:09:05ZengElsevierJournal of Functional Foods1756-46462024-04-01115106102Argininyl-fructosyl-glucose (AFG) antagonizes D-galactose-induced cellular senescence of neuro-2a via inhibiting endoplasmic reticulum stress and apoptosisShan Tang0Yun-yi Hou1Rui-yi Hu2Jun-jie Zhang3Xu-fei Gao4Ming Zhang5Zi Wang6Rui Zhang7Pin-hui Jia8Wei Li9College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China; College of Life Sciences, Engineering Research Center of the Chinese Ministry of Education for Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, China; Jilin Provincial International Joint Research Center for the Development and Utilization of Authentic Medicinal Materials, Changchun 130118, ChinaCollege of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, ChinaCollege of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, ChinaCollege of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, ChinaCollege of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, ChinaCollege of Medicine, Jilin University, Changchun 130021, ChinaCollege of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China; Jilin Provincial International Joint Research Center for the Development and Utilization of Authentic Medicinal Materials, Changchun 130118, ChinaCollege of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China; Corresponding authors at: College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China.College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China; Jilin Provincial International Joint Research Center for the Development and Utilization of Authentic Medicinal Materials, Changchun 130118, China; Corresponding authors at: College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China.College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China; College of Life Sciences, Engineering Research Center of the Chinese Ministry of Education for Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, China; Jilin Provincial International Joint Research Center for the Development and Utilization of Authentic Medicinal Materials, Changchun 130118, China; Corresponding authors at: College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China.Argininyl-fructosyl-glucose (AFG), a distinctive non-saponin compound isolated from red ginseng (Panax ginseng. C.A. Meyer), demonstrates neuroprotective effects. However, the molecular mechanism underlying AFG's anti-aging properties remains unclear. This study established an in vitro D-galactose-induced N2a cells subacute aging model to investigate AFG's anti-aging activity and potential mechanisms. Results indicated that AFG treatment (8 μM) significantly reduces the overexpression of aging-related proteins (p53/p21/p16) induced by D-galactose, highlighting its anti-aging efficacy. AFG concurrently diminishes ROS accumulation, lowering extracellular lactate dehydrogenase and alleviating intracellular oxidative stress. Notably, AFG suppresses the PERK/CHOP/caspase 12 pathway, reducing intracellular Ca2+ concentration and mitigating endoplasmic reticulum (ER) stress. Additionally, AFG decreases Bax and caspase 3 expression, elevates Bcl-2 and mitochondrial membrane potential, mitigating apoptosis. NAC with 4-PBA validated the results. Overall, AFG protects N2a cells from aging by reducing ER stress and apoptosis, suggesting its potential as a key substance for treating aging and neurodegenerative diseases.http://www.sciencedirect.com/science/article/pii/S175646462400104XArgininyl-fructosyl-glucoseGinsengD-galactose, N2a cellSenescenceEndoplasmic reticulum stressApoptosis |
| spellingShingle | Shan Tang Yun-yi Hou Rui-yi Hu Jun-jie Zhang Xu-fei Gao Ming Zhang Zi Wang Rui Zhang Pin-hui Jia Wei Li Argininyl-fructosyl-glucose (AFG) antagonizes D-galactose-induced cellular senescence of neuro-2a via inhibiting endoplasmic reticulum stress and apoptosis Journal of Functional Foods Argininyl-fructosyl-glucose Ginseng D-galactose, N2a cell Senescence Endoplasmic reticulum stress Apoptosis |
| title | Argininyl-fructosyl-glucose (AFG) antagonizes D-galactose-induced cellular senescence of neuro-2a via inhibiting endoplasmic reticulum stress and apoptosis |
| title_full | Argininyl-fructosyl-glucose (AFG) antagonizes D-galactose-induced cellular senescence of neuro-2a via inhibiting endoplasmic reticulum stress and apoptosis |
| title_fullStr | Argininyl-fructosyl-glucose (AFG) antagonizes D-galactose-induced cellular senescence of neuro-2a via inhibiting endoplasmic reticulum stress and apoptosis |
| title_full_unstemmed | Argininyl-fructosyl-glucose (AFG) antagonizes D-galactose-induced cellular senescence of neuro-2a via inhibiting endoplasmic reticulum stress and apoptosis |
| title_short | Argininyl-fructosyl-glucose (AFG) antagonizes D-galactose-induced cellular senescence of neuro-2a via inhibiting endoplasmic reticulum stress and apoptosis |
| title_sort | argininyl fructosyl glucose afg antagonizes d galactose induced cellular senescence of neuro 2a via inhibiting endoplasmic reticulum stress and apoptosis |
| topic | Argininyl-fructosyl-glucose Ginseng D-galactose, N2a cell Senescence Endoplasmic reticulum stress Apoptosis |
| url | http://www.sciencedirect.com/science/article/pii/S175646462400104X |
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