The Transcriptomic Analysis of NSC-34 Motor Neuron-Like Cells Reveals That Cannabigerol Influences Synaptic Pathways: A Comparative Study with Cannabidiol
More than 120 cannabinoids were isolated from <i>Cannabis sativa</i>. In particular, Cannabidiol (CBD) and Cannabigerol (CBG) represent the two most studied non-psychoactive cannabinoids. However, CBG is less studied and less data are available on its biological properties and influence...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-10-01
|
| Series: | Life |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-1729/10/10/227 |
| _version_ | 1797552101822300160 |
|---|---|
| author | Agnese Gugliandolo Serena Silvestro Luigi Chiricosta Federica Pollastro Placido Bramanti Emanuela Mazzon |
| author_facet | Agnese Gugliandolo Serena Silvestro Luigi Chiricosta Federica Pollastro Placido Bramanti Emanuela Mazzon |
| author_sort | Agnese Gugliandolo |
| collection | DOAJ |
| description | More than 120 cannabinoids were isolated from <i>Cannabis sativa</i>. In particular, Cannabidiol (CBD) and Cannabigerol (CBG) represent the two most studied non-psychoactive cannabinoids. However, CBG is less studied and less data are available on its biological properties and influence on synaptic transmission. On the contrary, CBD is already known to modulate brain excitatory glutamate, inhibitory γ-aminobutyric acid (GABA) and dopamine neurotransmission. In this study, using Next-Generation Sequencing (NGS) technology, we evaluated how CBG (1 or 5 µM) and CBD (1 or 5 µM) influence the transcriptome of the main neurotransmission pathways in NSC-34 motor neuron-like cells. At first, we evaluated that CBG and CBD were not cytotoxic and decreased the expression of pro-apoptotic genes. CBG and CBD are able to influence the expression of the genes involved in glutamate, GABA and dopamine signaling. Interestingly, the transcriptional changes induced by CBG were similar compared to CBD. |
| first_indexed | 2024-03-10T15:55:11Z |
| format | Article |
| id | doaj.art-a61b40308eff48838ed95cb861d4da0e |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-10T15:55:11Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-a61b40308eff48838ed95cb861d4da0e2023-11-20T15:44:28ZengMDPI AGLife2075-17292020-10-01101022710.3390/life10100227The Transcriptomic Analysis of NSC-34 Motor Neuron-Like Cells Reveals That Cannabigerol Influences Synaptic Pathways: A Comparative Study with CannabidiolAgnese Gugliandolo0Serena Silvestro1Luigi Chiricosta2Federica Pollastro3Placido Bramanti4Emanuela Mazzon5IRCCS Centro Neurolesi “Bonino-Pulejo”, Via Provinciale Palermo, Contrada Casazza, 98124 Messina, ItalyIRCCS Centro Neurolesi “Bonino-Pulejo”, Via Provinciale Palermo, Contrada Casazza, 98124 Messina, ItalyIRCCS Centro Neurolesi “Bonino-Pulejo”, Via Provinciale Palermo, Contrada Casazza, 98124 Messina, ItalyDepartment of Pharmaceutical Sciences, University of Eastern Piedmont, Largo Donegani 2, 28100 Novara, ItalyIRCCS Centro Neurolesi “Bonino-Pulejo”, Via Provinciale Palermo, Contrada Casazza, 98124 Messina, ItalyIRCCS Centro Neurolesi “Bonino-Pulejo”, Via Provinciale Palermo, Contrada Casazza, 98124 Messina, ItalyMore than 120 cannabinoids were isolated from <i>Cannabis sativa</i>. In particular, Cannabidiol (CBD) and Cannabigerol (CBG) represent the two most studied non-psychoactive cannabinoids. However, CBG is less studied and less data are available on its biological properties and influence on synaptic transmission. On the contrary, CBD is already known to modulate brain excitatory glutamate, inhibitory γ-aminobutyric acid (GABA) and dopamine neurotransmission. In this study, using Next-Generation Sequencing (NGS) technology, we evaluated how CBG (1 or 5 µM) and CBD (1 or 5 µM) influence the transcriptome of the main neurotransmission pathways in NSC-34 motor neuron-like cells. At first, we evaluated that CBG and CBD were not cytotoxic and decreased the expression of pro-apoptotic genes. CBG and CBD are able to influence the expression of the genes involved in glutamate, GABA and dopamine signaling. Interestingly, the transcriptional changes induced by CBG were similar compared to CBD.https://www.mdpi.com/2075-1729/10/10/227CannabidiolCannabigerolGlutamatergic synapsesGABAergic synapsesmotor neuron-like cellstranscriptomic analysis |
| spellingShingle | Agnese Gugliandolo Serena Silvestro Luigi Chiricosta Federica Pollastro Placido Bramanti Emanuela Mazzon The Transcriptomic Analysis of NSC-34 Motor Neuron-Like Cells Reveals That Cannabigerol Influences Synaptic Pathways: A Comparative Study with Cannabidiol Life Cannabidiol Cannabigerol Glutamatergic synapses GABAergic synapses motor neuron-like cells transcriptomic analysis |
| title | The Transcriptomic Analysis of NSC-34 Motor Neuron-Like Cells Reveals That Cannabigerol Influences Synaptic Pathways: A Comparative Study with Cannabidiol |
| title_full | The Transcriptomic Analysis of NSC-34 Motor Neuron-Like Cells Reveals That Cannabigerol Influences Synaptic Pathways: A Comparative Study with Cannabidiol |
| title_fullStr | The Transcriptomic Analysis of NSC-34 Motor Neuron-Like Cells Reveals That Cannabigerol Influences Synaptic Pathways: A Comparative Study with Cannabidiol |
| title_full_unstemmed | The Transcriptomic Analysis of NSC-34 Motor Neuron-Like Cells Reveals That Cannabigerol Influences Synaptic Pathways: A Comparative Study with Cannabidiol |
| title_short | The Transcriptomic Analysis of NSC-34 Motor Neuron-Like Cells Reveals That Cannabigerol Influences Synaptic Pathways: A Comparative Study with Cannabidiol |
| title_sort | transcriptomic analysis of nsc 34 motor neuron like cells reveals that cannabigerol influences synaptic pathways a comparative study with cannabidiol |
| topic | Cannabidiol Cannabigerol Glutamatergic synapses GABAergic synapses motor neuron-like cells transcriptomic analysis |
| url | https://www.mdpi.com/2075-1729/10/10/227 |
| work_keys_str_mv | AT agnesegugliandolo thetranscriptomicanalysisofnsc34motorneuronlikecellsrevealsthatcannabigerolinfluencessynapticpathwaysacomparativestudywithcannabidiol AT serenasilvestro thetranscriptomicanalysisofnsc34motorneuronlikecellsrevealsthatcannabigerolinfluencessynapticpathwaysacomparativestudywithcannabidiol AT luigichiricosta thetranscriptomicanalysisofnsc34motorneuronlikecellsrevealsthatcannabigerolinfluencessynapticpathwaysacomparativestudywithcannabidiol AT federicapollastro thetranscriptomicanalysisofnsc34motorneuronlikecellsrevealsthatcannabigerolinfluencessynapticpathwaysacomparativestudywithcannabidiol AT placidobramanti thetranscriptomicanalysisofnsc34motorneuronlikecellsrevealsthatcannabigerolinfluencessynapticpathwaysacomparativestudywithcannabidiol AT emanuelamazzon thetranscriptomicanalysisofnsc34motorneuronlikecellsrevealsthatcannabigerolinfluencessynapticpathwaysacomparativestudywithcannabidiol AT agnesegugliandolo transcriptomicanalysisofnsc34motorneuronlikecellsrevealsthatcannabigerolinfluencessynapticpathwaysacomparativestudywithcannabidiol AT serenasilvestro transcriptomicanalysisofnsc34motorneuronlikecellsrevealsthatcannabigerolinfluencessynapticpathwaysacomparativestudywithcannabidiol AT luigichiricosta transcriptomicanalysisofnsc34motorneuronlikecellsrevealsthatcannabigerolinfluencessynapticpathwaysacomparativestudywithcannabidiol AT federicapollastro transcriptomicanalysisofnsc34motorneuronlikecellsrevealsthatcannabigerolinfluencessynapticpathwaysacomparativestudywithcannabidiol AT placidobramanti transcriptomicanalysisofnsc34motorneuronlikecellsrevealsthatcannabigerolinfluencessynapticpathwaysacomparativestudywithcannabidiol AT emanuelamazzon transcriptomicanalysisofnsc34motorneuronlikecellsrevealsthatcannabigerolinfluencessynapticpathwaysacomparativestudywithcannabidiol |