Form-Dependent Toxicity of Silver Nanomaterials in Rainbow Trout Gills
The toxicity of the form of nanoparticles is presently not well understood. The purpose of this study consists in comparing the toxicity of various forms of silver nanoparticles (nAg) in juvenile rainbow trout <i>Oncorhynchus mykiss</i>. Juveniles were exposed to various forms of polyvin...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-04-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/13/8/1356 |
| _version_ | 1797604043331207168 |
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| author | Joëlle Auclair Patrice Turcotte Christian Gagnon Caroline Peyrot Kevin James Wilkinson François Gagné |
| author_facet | Joëlle Auclair Patrice Turcotte Christian Gagnon Caroline Peyrot Kevin James Wilkinson François Gagné |
| author_sort | Joëlle Auclair |
| collection | DOAJ |
| description | The toxicity of the form of nanoparticles is presently not well understood. The purpose of this study consists in comparing the toxicity of various forms of silver nanoparticles (nAg) in juvenile rainbow trout <i>Oncorhynchus mykiss</i>. Juveniles were exposed to various forms of polyvinyl-coated nAg of similar size for 96 h at 15 °C. After the exposure period, the gills were isolated and analyzed for Ag uptake/distribution, oxidative stress, glucose metabolism, and genotoxicity. Higher levels of Ag were detected in gills in fish exposed to dissolved Ag followed by spherical, cubic, and prismatic nAg. Size-exclusion chromatography of gill fractions revealed that the dissolution of nAg was observed for all forms of nAg where prismatic nAg released more important levels of Ag in the protein pool as in fish exposed to dissolved Ag as well. The aggregation of nAg was more important for cubic nAg in respect of the other forms of nAg. The data revealed that lipid peroxidation was closely associated with protein aggregation and viscosity. Biomarkers revealed changes in lipid/oxidative stress and genotoxicity, which were related to the loss of protein aggregation and inflammation (NO<sub>2</sub> levels), respectively. In general, the observed effects were found for all forms of nAg where the effects from prismatic nAg were generally higher than for spherical and cubic nAg. The strong relationship between genotoxicity and inflammation response suggests the participation of the immune system in the observed responses of juvenile fish gills. |
| first_indexed | 2024-03-11T04:40:35Z |
| format | Article |
| id | doaj.art-a61f65a3b35b4ab288ac507f6197af59 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-11T04:40:35Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-a61f65a3b35b4ab288ac507f6197af592023-11-17T20:43:19ZengMDPI AGNanomaterials2079-49912023-04-01138135610.3390/nano13081356Form-Dependent Toxicity of Silver Nanomaterials in Rainbow Trout GillsJoëlle Auclair0Patrice Turcotte1Christian Gagnon2Caroline Peyrot3Kevin James Wilkinson4François Gagné5Aquatic Contaminants Research Division, Environment and Climate Change Canada, Montreal, QC H2Y 2E7, CanadaAquatic Contaminants Research Division, Environment and Climate Change Canada, Montreal, QC H2Y 2E7, CanadaAquatic Contaminants Research Division, Environment and Climate Change Canada, Montreal, QC H2Y 2E7, CanadaChemistry Department, Montréal University, Montreal, QC H3C 3J7, CanadaChemistry Department, Montréal University, Montreal, QC H3C 3J7, CanadaAquatic Contaminants Research Division, Environment and Climate Change Canada, Montreal, QC H2Y 2E7, CanadaThe toxicity of the form of nanoparticles is presently not well understood. The purpose of this study consists in comparing the toxicity of various forms of silver nanoparticles (nAg) in juvenile rainbow trout <i>Oncorhynchus mykiss</i>. Juveniles were exposed to various forms of polyvinyl-coated nAg of similar size for 96 h at 15 °C. After the exposure period, the gills were isolated and analyzed for Ag uptake/distribution, oxidative stress, glucose metabolism, and genotoxicity. Higher levels of Ag were detected in gills in fish exposed to dissolved Ag followed by spherical, cubic, and prismatic nAg. Size-exclusion chromatography of gill fractions revealed that the dissolution of nAg was observed for all forms of nAg where prismatic nAg released more important levels of Ag in the protein pool as in fish exposed to dissolved Ag as well. The aggregation of nAg was more important for cubic nAg in respect of the other forms of nAg. The data revealed that lipid peroxidation was closely associated with protein aggregation and viscosity. Biomarkers revealed changes in lipid/oxidative stress and genotoxicity, which were related to the loss of protein aggregation and inflammation (NO<sub>2</sub> levels), respectively. In general, the observed effects were found for all forms of nAg where the effects from prismatic nAg were generally higher than for spherical and cubic nAg. The strong relationship between genotoxicity and inflammation response suggests the participation of the immune system in the observed responses of juvenile fish gills.https://www.mdpi.com/2079-4991/13/8/1356silver nanoparticleformavailabilitydissolutionoxidative stresslipids |
| spellingShingle | Joëlle Auclair Patrice Turcotte Christian Gagnon Caroline Peyrot Kevin James Wilkinson François Gagné Form-Dependent Toxicity of Silver Nanomaterials in Rainbow Trout Gills Nanomaterials silver nanoparticle form availability dissolution oxidative stress lipids |
| title | Form-Dependent Toxicity of Silver Nanomaterials in Rainbow Trout Gills |
| title_full | Form-Dependent Toxicity of Silver Nanomaterials in Rainbow Trout Gills |
| title_fullStr | Form-Dependent Toxicity of Silver Nanomaterials in Rainbow Trout Gills |
| title_full_unstemmed | Form-Dependent Toxicity of Silver Nanomaterials in Rainbow Trout Gills |
| title_short | Form-Dependent Toxicity of Silver Nanomaterials in Rainbow Trout Gills |
| title_sort | form dependent toxicity of silver nanomaterials in rainbow trout gills |
| topic | silver nanoparticle form availability dissolution oxidative stress lipids |
| url | https://www.mdpi.com/2079-4991/13/8/1356 |
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