Effect of Mammalian Tissue Source on the Molecular and Macroscopic Characteristics of UV-Cured Type I Collagen Hydrogel Networks
The tissue source of type I collagen is critical to ensure scalability and regulation-friendly clinical translation of new medical device prototypes. However, the selection of a commercial source of collagen that fulfils both aforementioned requirements and is compliant with new manufacturing routes...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-01-01
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| Series: | Prosthesis |
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| Online Access: | https://www.mdpi.com/2673-1592/4/1/1 |
| _version_ | 1818771984335503360 |
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| author | Charles Brooker Giuseppe Tronci |
| author_facet | Charles Brooker Giuseppe Tronci |
| author_sort | Charles Brooker |
| collection | DOAJ |
| description | The tissue source of type I collagen is critical to ensure scalability and regulation-friendly clinical translation of new medical device prototypes. However, the selection of a commercial source of collagen that fulfils both aforementioned requirements and is compliant with new manufacturing routes is challenging. This study investigates the effect that type I collagen extracted from three different mammalian tissues has on the molecular and macroscopic characteristics of a new UV-cured collagen hydrogel. Pepsin-solubilised bovine atelocollagen (BA) and pepsin-solubilised porcine atelocollagen (PA) were selected as commercially available raw materials associated with varying safety risks and compared with in-house acid-extracted type I collagen from rat tails (CRT). All raw materials displayed the typical dichroic and electrophoretic characteristics of type I collagen, while significantly decreased lysine content was measured on samples of PA. Following covalent functionalisation with 4-vinylbenzyl chloride (4VBC), BA and CRT products generated comparable UV-cured hydrogels with significantly increased averaged gel content (<i>G</i> ≥ 97 wt.%), while the porcine variants revealed the highest swelling ratio (<i>SR</i> = 2224 ± 242 wt.%) and an order of magnitude reduction in compression modulus (<i>E<sub>c</sub></i> = 6 ± 2 kPa). Collectively, these results support the use of bovine tissues as a chemically viable source of type I collagen for the realisation of UV-cured hydrogels with competitive mechanical properties and covalent network architectures. |
| first_indexed | 2024-12-18T10:02:08Z |
| format | Article |
| id | doaj.art-a6203b44c57b4e47a7aa5c7ad6a85e40 |
| institution | Directory Open Access Journal |
| issn | 2673-1592 |
| language | English |
| last_indexed | 2024-12-18T10:02:08Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Prosthesis |
| spelling | doaj.art-a6203b44c57b4e47a7aa5c7ad6a85e402022-12-21T21:11:37ZengMDPI AGProsthesis2673-15922022-01-014111410.3390/prosthesis4010001Effect of Mammalian Tissue Source on the Molecular and Macroscopic Characteristics of UV-Cured Type I Collagen Hydrogel NetworksCharles Brooker0Giuseppe Tronci1Clothworkers’ Centre for Textile Materials Innovation for Healthcare (CCTMIH), School of Design, University of Leeds, Leeds LS2 9JT, UKClothworkers’ Centre for Textile Materials Innovation for Healthcare (CCTMIH), School of Design, University of Leeds, Leeds LS2 9JT, UKThe tissue source of type I collagen is critical to ensure scalability and regulation-friendly clinical translation of new medical device prototypes. However, the selection of a commercial source of collagen that fulfils both aforementioned requirements and is compliant with new manufacturing routes is challenging. This study investigates the effect that type I collagen extracted from three different mammalian tissues has on the molecular and macroscopic characteristics of a new UV-cured collagen hydrogel. Pepsin-solubilised bovine atelocollagen (BA) and pepsin-solubilised porcine atelocollagen (PA) were selected as commercially available raw materials associated with varying safety risks and compared with in-house acid-extracted type I collagen from rat tails (CRT). All raw materials displayed the typical dichroic and electrophoretic characteristics of type I collagen, while significantly decreased lysine content was measured on samples of PA. Following covalent functionalisation with 4-vinylbenzyl chloride (4VBC), BA and CRT products generated comparable UV-cured hydrogels with significantly increased averaged gel content (<i>G</i> ≥ 97 wt.%), while the porcine variants revealed the highest swelling ratio (<i>SR</i> = 2224 ± 242 wt.%) and an order of magnitude reduction in compression modulus (<i>E<sub>c</sub></i> = 6 ± 2 kPa). Collectively, these results support the use of bovine tissues as a chemically viable source of type I collagen for the realisation of UV-cured hydrogels with competitive mechanical properties and covalent network architectures.https://www.mdpi.com/2673-1592/4/1/1type I collagenbovinemammalian tissueUV-cured hydrogelmechanical propertieslysine content |
| spellingShingle | Charles Brooker Giuseppe Tronci Effect of Mammalian Tissue Source on the Molecular and Macroscopic Characteristics of UV-Cured Type I Collagen Hydrogel Networks Prosthesis type I collagen bovine mammalian tissue UV-cured hydrogel mechanical properties lysine content |
| title | Effect of Mammalian Tissue Source on the Molecular and Macroscopic Characteristics of UV-Cured Type I Collagen Hydrogel Networks |
| title_full | Effect of Mammalian Tissue Source on the Molecular and Macroscopic Characteristics of UV-Cured Type I Collagen Hydrogel Networks |
| title_fullStr | Effect of Mammalian Tissue Source on the Molecular and Macroscopic Characteristics of UV-Cured Type I Collagen Hydrogel Networks |
| title_full_unstemmed | Effect of Mammalian Tissue Source on the Molecular and Macroscopic Characteristics of UV-Cured Type I Collagen Hydrogel Networks |
| title_short | Effect of Mammalian Tissue Source on the Molecular and Macroscopic Characteristics of UV-Cured Type I Collagen Hydrogel Networks |
| title_sort | effect of mammalian tissue source on the molecular and macroscopic characteristics of uv cured type i collagen hydrogel networks |
| topic | type I collagen bovine mammalian tissue UV-cured hydrogel mechanical properties lysine content |
| url | https://www.mdpi.com/2673-1592/4/1/1 |
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