Isolation and Characterisation of Endophytic Bacteria from Holostemma ada-kodien Schult
Plants with medical properties are often enriched with endophytes that have the potential to produce important bioactive compounds. Endophytes after entering the plant tissue may either colonize a particular tissue or may spread throughout the host plant without causing damage. The possession o...
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| Format: | Article |
| Language: | English |
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Journal of Pure and Applied Microbiology
2020-09-01
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| Series: | Journal of Pure and Applied Microbiology |
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| Online Access: | https://microbiologyjournal.org/isolation-and-characterisation-of-endophytic-bacteria-from-holostemma-ada-kodien-schult/ |
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| author | Pooja Pushkaran Dinta Daniel Sivaramakrishnan Subramaniyan G.S. Sandhia |
| author_facet | Pooja Pushkaran Dinta Daniel Sivaramakrishnan Subramaniyan G.S. Sandhia |
| author_sort | Pooja Pushkaran |
| collection | DOAJ |
| description | Plants with medical properties are often enriched with endophytes that have the potential to produce important bioactive compounds. Endophytes after entering the plant tissue may either colonize a particular tissue or may spread throughout the host plant without causing damage. The possession of pharmaceutical and biological properties has made the Holostemma ada-kodien Schult as one of the widely used plants of medicinal importance in India. Following the direct cut method three endophytic bacterial strains (UC H1, UC H4 and UC H7) were isolated, identified and characterized from the healthy looking rhizome of H. ada-kodien. Among these isolates, UC H1 and UC H4 were found to have many properties like antibacterial compounds, hydrolytic enzymes and plant growth promoting traits. The isolate UC H4 have ability for Indole-3-Acetic Acid (IAA) production of 513.54 U/ml and very good protease and pectinase activities of 20.65 U/ml and 16.09 U/ml respectively. So far no reports are available on the endophytic microflora of H. ada-kodien. |
| first_indexed | 2024-12-17T13:00:27Z |
| format | Article |
| id | doaj.art-a6248efd2b094384bc1be1e62a967911 |
| institution | Directory Open Access Journal |
| issn | 0973-7510 2581-690X |
| language | English |
| last_indexed | 2024-12-17T13:00:27Z |
| publishDate | 2020-09-01 |
| publisher | Journal of Pure and Applied Microbiology |
| record_format | Article |
| series | Journal of Pure and Applied Microbiology |
| spelling | doaj.art-a6248efd2b094384bc1be1e62a9679112022-12-21T21:47:23ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2020-09-011431949196010.22207/JPAM.14.3.34Isolation and Characterisation of Endophytic Bacteria from Holostemma ada-kodien SchultPooja Pushkaranhttps://orcid.org/0000-0002-0970-6128Dinta Danielhttps://orcid.org/0000-0002-6587-4898Sivaramakrishnan Subramaniyanhttps://orcid.org/0000-0002-3911-940XG.S. Sandhiahttps://orcid.org/0000-0001-6039-4337Plants with medical properties are often enriched with endophytes that have the potential to produce important bioactive compounds. Endophytes after entering the plant tissue may either colonize a particular tissue or may spread throughout the host plant without causing damage. The possession of pharmaceutical and biological properties has made the Holostemma ada-kodien Schult as one of the widely used plants of medicinal importance in India. Following the direct cut method three endophytic bacterial strains (UC H1, UC H4 and UC H7) were isolated, identified and characterized from the healthy looking rhizome of H. ada-kodien. Among these isolates, UC H1 and UC H4 were found to have many properties like antibacterial compounds, hydrolytic enzymes and plant growth promoting traits. The isolate UC H4 have ability for Indole-3-Acetic Acid (IAA) production of 513.54 U/ml and very good protease and pectinase activities of 20.65 U/ml and 16.09 U/ml respectively. So far no reports are available on the endophytic microflora of H. ada-kodien.https://microbiologyjournal.org/isolation-and-characterisation-of-endophytic-bacteria-from-holostemma-ada-kodien-schult/endophytic bacteria16s rrna gene sequencingantibacterial activityiaa productionrp-hplcphosphate solubilisationacc deaminase production |
| spellingShingle | Pooja Pushkaran Dinta Daniel Sivaramakrishnan Subramaniyan G.S. Sandhia Isolation and Characterisation of Endophytic Bacteria from Holostemma ada-kodien Schult Journal of Pure and Applied Microbiology endophytic bacteria 16s rrna gene sequencing antibacterial activity iaa production rp-hplc phosphate solubilisation acc deaminase production |
| title | Isolation and Characterisation of Endophytic Bacteria from Holostemma ada-kodien Schult |
| title_full | Isolation and Characterisation of Endophytic Bacteria from Holostemma ada-kodien Schult |
| title_fullStr | Isolation and Characterisation of Endophytic Bacteria from Holostemma ada-kodien Schult |
| title_full_unstemmed | Isolation and Characterisation of Endophytic Bacteria from Holostemma ada-kodien Schult |
| title_short | Isolation and Characterisation of Endophytic Bacteria from Holostemma ada-kodien Schult |
| title_sort | isolation and characterisation of endophytic bacteria from holostemma ada kodien schult |
| topic | endophytic bacteria 16s rrna gene sequencing antibacterial activity iaa production rp-hplc phosphate solubilisation acc deaminase production |
| url | https://microbiologyjournal.org/isolation-and-characterisation-of-endophytic-bacteria-from-holostemma-ada-kodien-schult/ |
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