Plasmid Profile Analysis and bla VIM Gene Detection of Metalo β-lactamase (MBL) Producing Pseudomonas aeruginosa Isolates from Clinical Samples
Introduction: Pseudomonas aeruginosa is a frequent colonizer of hospitalized patients. They are responsible for serious infections such as meningitis, urological infections, septicemia and pneumonia. Carbapenem resistance of Pseudomonas aeruginosa is currently increasingly reported which is often...
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| Format: | Article |
| Language: | English |
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JCDR Research and Publications Private Limited
2014-06-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/4492/8096_CE(RA)_PF1(AG)_F(T)_PFA(AK)_PF2(PAG)_PF2(PN).pdf |
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| author | Jayanthi S. Jeya M. |
| author_facet | Jayanthi S. Jeya M. |
| author_sort | Jayanthi S. |
| collection | DOAJ |
| description | Introduction: Pseudomonas aeruginosa is a frequent colonizer of
hospitalized patients. They are responsible for serious infections
such as meningitis, urological infections, septicemia and pneumonia. Carbapenem resistance of Pseudomonas aeruginosa
is currently increasingly reported which is often mediated by
production of metallo-β-lactamase (MBL). Multidrug resistant
Pseudomonas aeruginosa isolates may involve reduced cell wall
permeability, production of chromosomal and plasmid mediated
β lactamases, aminoglycosides modifying enzymes and an active
multidrug efflux mechanism.
Objective: This study is aimed to detect the presence and
the nature of plasmids among metallo-β-lactamase producing
Pseudomonas aeruginosa isolates. Also to detect the presence of
bla VIM gene from these isolates.
Materials and Methods: Clinical isolates of Pseudomonas
aeruginosa showing the metalo-β-lactamase enzyme (MBL)
production were isolated. The MBL production was confirmed
by three different methods. From the MBL producing isolates
plasmid extraction was done by alkaline lysis method. Plasmid
positive isolates were subjected for blaVIM gene detection by PCR
method.
Results: Two thousand seventy six clinical samples yielded 316
(15.22%) Pseudomonas aeruginosa isolates, out of which 141
(44.62%) were multidrug resistant. Among them 25 (17.73%)
were metallo-β-lactamase enzyme producers. Plasmids were
extracted from 18 out of 25 isolates tested. Five out of 18
isolates were positive for the blaVIM gene detection by the PCR
amplification.
Conclusion: The MBL producers were susceptible to polymyxin
/colistin with MIC ranging from 0.5 – 2µg/ml. Molecular detection
of specific genes bla VIM were positive among the carbapenem
resistant isolates. |
| first_indexed | 2024-12-22T06:09:22Z |
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| id | doaj.art-a6258f11caa14f75a619224ea6f046ca |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-22T06:09:22Z |
| publishDate | 2014-06-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-a6258f11caa14f75a619224ea6f046ca2022-12-21T18:36:17ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2014-06-0186DC16DC1910.7860/JCDR/2014/8096.4492Plasmid Profile Analysis and bla VIM Gene Detection of Metalo β-lactamase (MBL) Producing Pseudomonas aeruginosa Isolates from Clinical SamplesJayanthi S.0Jeya M.1Associate Professor, Department of Microbiology, Chettinad Hospital &Research Institute, Rajiv Gandhi Salai, Kelambakkam, Kanchipuram District, Chennai, Tamil Nadu, India.Professor, Head of Department, Department of Microbiology, Chettinad Hospital &Research Institute, Rajiv Gandhi salai, Kelambakkam, Kanchipuram District, Chennai, Tamil Nadu, India.Introduction: Pseudomonas aeruginosa is a frequent colonizer of hospitalized patients. They are responsible for serious infections such as meningitis, urological infections, septicemia and pneumonia. Carbapenem resistance of Pseudomonas aeruginosa is currently increasingly reported which is often mediated by production of metallo-β-lactamase (MBL). Multidrug resistant Pseudomonas aeruginosa isolates may involve reduced cell wall permeability, production of chromosomal and plasmid mediated β lactamases, aminoglycosides modifying enzymes and an active multidrug efflux mechanism. Objective: This study is aimed to detect the presence and the nature of plasmids among metallo-β-lactamase producing Pseudomonas aeruginosa isolates. Also to detect the presence of bla VIM gene from these isolates. Materials and Methods: Clinical isolates of Pseudomonas aeruginosa showing the metalo-β-lactamase enzyme (MBL) production were isolated. The MBL production was confirmed by three different methods. From the MBL producing isolates plasmid extraction was done by alkaline lysis method. Plasmid positive isolates were subjected for blaVIM gene detection by PCR method. Results: Two thousand seventy six clinical samples yielded 316 (15.22%) Pseudomonas aeruginosa isolates, out of which 141 (44.62%) were multidrug resistant. Among them 25 (17.73%) were metallo-β-lactamase enzyme producers. Plasmids were extracted from 18 out of 25 isolates tested. Five out of 18 isolates were positive for the blaVIM gene detection by the PCR amplification. Conclusion: The MBL producers were susceptible to polymyxin /colistin with MIC ranging from 0.5 – 2µg/ml. Molecular detection of specific genes bla VIM were positive among the carbapenem resistant isolates.https://jcdr.net/articles/PDF/4492/8096_CE(RA)_PF1(AG)_F(T)_PFA(AK)_PF2(PAG)_PF2(PN).pdfblavimcarbapenem resistancemetallo-β-lactamase enzyme producerspseudomonas aeruginosaplasmids |
| spellingShingle | Jayanthi S. Jeya M. Plasmid Profile Analysis and bla VIM Gene Detection of Metalo β-lactamase (MBL) Producing Pseudomonas aeruginosa Isolates from Clinical Samples Journal of Clinical and Diagnostic Research blavim carbapenem resistance metallo-β-lactamase enzyme producers pseudomonas aeruginosa plasmids |
| title | Plasmid Profile Analysis and bla VIM Gene Detection of Metalo β-lactamase (MBL) Producing Pseudomonas aeruginosa Isolates from Clinical Samples |
| title_full | Plasmid Profile Analysis and bla VIM Gene Detection of Metalo β-lactamase (MBL) Producing Pseudomonas aeruginosa Isolates from Clinical Samples |
| title_fullStr | Plasmid Profile Analysis and bla VIM Gene Detection of Metalo β-lactamase (MBL) Producing Pseudomonas aeruginosa Isolates from Clinical Samples |
| title_full_unstemmed | Plasmid Profile Analysis and bla VIM Gene Detection of Metalo β-lactamase (MBL) Producing Pseudomonas aeruginosa Isolates from Clinical Samples |
| title_short | Plasmid Profile Analysis and bla VIM Gene Detection of Metalo β-lactamase (MBL) Producing Pseudomonas aeruginosa Isolates from Clinical Samples |
| title_sort | plasmid profile analysis and bla vim gene detection of metalo β lactamase mbl producing pseudomonas aeruginosa isolates from clinical samples |
| topic | blavim carbapenem resistance metallo-β-lactamase enzyme producers pseudomonas aeruginosa plasmids |
| url | https://jcdr.net/articles/PDF/4492/8096_CE(RA)_PF1(AG)_F(T)_PFA(AK)_PF2(PAG)_PF2(PN).pdf |
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