Screening of in vitro-produced cattle embryos to assess incidence and characteristics of unbalanced chromosomal aberrations
In cattle, pregnancy rates of in vitro-produced embryos are lower than those of in vivo-produced embryos. One of the reasons may be the increase in chromosomal aberrations due to in vitro maturation and fertilization of the oocyte. Currently, embryo transfer is commonly applied in nucleus cattle bre...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-03-01
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| Series: | JDS Communications |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666910223000054 |
| _version_ | 1827909366039183360 |
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| author | Aniek C. Bouwman Erik Mullaart |
| author_facet | Aniek C. Bouwman Erik Mullaart |
| author_sort | Aniek C. Bouwman |
| collection | DOAJ |
| description | In cattle, pregnancy rates of in vitro-produced embryos are lower than those of in vivo-produced embryos. One of the reasons may be the increase in chromosomal aberrations due to in vitro maturation and fertilization of the oocyte. Currently, embryo transfer is commonly applied in nucleus cattle breeding programs, and the embryos are genotyped for genomic selection. Therefore, intensity data from SNP arrays can be exploited for preimplantation genetic testing by screening the intensity data of the embryos for unbalanced chromosomal aberrations. A total of 558 stage 8 Dutch Holstein embryos genotyped with SNP arrays were screened in an observational study in retrospect. We found a 5% incidence rate of unbalanced chromosomal aberrations (aneuploidy and ploidy issues) among 430 successfully genotyped cattle embryos. The 22 affected embryos showed either aneuploidy or ploidy issues; monosomy was most frequently observed (14/22). In most cases (16/19) the maternal chromosome or chromosomes were lost or gained. One of the monosomy cases gave rise to a live-born fully diploid individual, suggesting mosaicism. Given that embryo genotypes are readily available, monitoring incidence can easily be applied. Moreover, selection for euploid embryos may improve pregnancy rates for in vitro embryo transfer. |
| first_indexed | 2024-03-13T01:36:04Z |
| format | Article |
| id | doaj.art-a6265e30a1c742c29ba6fe5f898779d6 |
| institution | Directory Open Access Journal |
| issn | 2666-9102 |
| language | English |
| last_indexed | 2024-03-13T01:36:04Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | JDS Communications |
| spelling | doaj.art-a6265e30a1c742c29ba6fe5f898779d62023-07-04T05:11:14ZengElsevierJDS Communications2666-91022023-03-0142101105Screening of in vitro-produced cattle embryos to assess incidence and characteristics of unbalanced chromosomal aberrationsAniek C. Bouwman0Erik Mullaart1Animal Breeding & Genomics, Wageningen University & Research, PO Box 338, 6700 AH Wageningen, the Netherlands; Corresponding authorCRV B.V., Wassenaarweg 20, 6843 NW, Arnhem, the NetherlandsIn cattle, pregnancy rates of in vitro-produced embryos are lower than those of in vivo-produced embryos. One of the reasons may be the increase in chromosomal aberrations due to in vitro maturation and fertilization of the oocyte. Currently, embryo transfer is commonly applied in nucleus cattle breeding programs, and the embryos are genotyped for genomic selection. Therefore, intensity data from SNP arrays can be exploited for preimplantation genetic testing by screening the intensity data of the embryos for unbalanced chromosomal aberrations. A total of 558 stage 8 Dutch Holstein embryos genotyped with SNP arrays were screened in an observational study in retrospect. We found a 5% incidence rate of unbalanced chromosomal aberrations (aneuploidy and ploidy issues) among 430 successfully genotyped cattle embryos. The 22 affected embryos showed either aneuploidy or ploidy issues; monosomy was most frequently observed (14/22). In most cases (16/19) the maternal chromosome or chromosomes were lost or gained. One of the monosomy cases gave rise to a live-born fully diploid individual, suggesting mosaicism. Given that embryo genotypes are readily available, monitoring incidence can easily be applied. Moreover, selection for euploid embryos may improve pregnancy rates for in vitro embryo transfer.http://www.sciencedirect.com/science/article/pii/S2666910223000054 |
| spellingShingle | Aniek C. Bouwman Erik Mullaart Screening of in vitro-produced cattle embryos to assess incidence and characteristics of unbalanced chromosomal aberrations JDS Communications |
| title | Screening of in vitro-produced cattle embryos to assess incidence and characteristics of unbalanced chromosomal aberrations |
| title_full | Screening of in vitro-produced cattle embryos to assess incidence and characteristics of unbalanced chromosomal aberrations |
| title_fullStr | Screening of in vitro-produced cattle embryos to assess incidence and characteristics of unbalanced chromosomal aberrations |
| title_full_unstemmed | Screening of in vitro-produced cattle embryos to assess incidence and characteristics of unbalanced chromosomal aberrations |
| title_short | Screening of in vitro-produced cattle embryos to assess incidence and characteristics of unbalanced chromosomal aberrations |
| title_sort | screening of in vitro produced cattle embryos to assess incidence and characteristics of unbalanced chromosomal aberrations |
| url | http://www.sciencedirect.com/science/article/pii/S2666910223000054 |
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