Microsporidial Spores in Fecal Samples of Some Domesticated Animals Living in Giza, Egypt
Background: The aim of the present work was to investigate the prevalence and species of intestinal microsporidiosis among animals in Giza, Egypt. Methods: A total of 869 animal fecal samples were collected from domesticated animals (dogs, cats, rabbits, cattle, buffaloes, sheep, goats, donkeys and...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tehran University of Medical Sciences
2016-12-01
|
| Series: | Iranian Journal of Parasitology |
| Subjects: | |
| Online Access: | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/1059 |
| _version_ | 1819242284232736768 |
|---|---|
| author | Ahmad Z. AL-HERRAWY Mahmoud A. GAD |
| author_facet | Ahmad Z. AL-HERRAWY Mahmoud A. GAD |
| author_sort | Ahmad Z. AL-HERRAWY |
| collection | DOAJ |
| description | Background: The aim of the present work was to investigate the prevalence and species of intestinal microsporidiosis among animals in Giza, Egypt.
Methods: A total of 869 animal fecal samples were collected from domesticated animals (dogs, cats, rabbits, cattle, buffaloes, sheep, goats, donkeys and pigs) living in Giza, Egypt. Spores of microsporidia were concentrated from collected samples by centrifugation and finally stained with modified trichrome (MT) stain to detect microsporidial spores. Microsporidial spores in microscopically-positive samples were molecularly confirmed and identified using species-specific primers.
Results: Spores of microsporidia were microscopically detected in 17.0% of the examined animal fecal samples. The highest and lowest rates of infection with intestinal microsporidia were recorded in dogs (33.3%) and buffaloes (6.9%), respectively. Molecularly, the obtained microsporidial spores were classified as Enterocytozoon bieneusi and E. intestinalis. Dual infection with both identified species was observed in fecal samples from buffalo, rabbit, goat, cat, pig and dog.
Conclusion: Domestic animals may play a role in dissemination of intestinal microsporidiosis in the environment. Examined animals were infected with E. bieneusi in a higher percentage than E. intestinalis. |
| first_indexed | 2024-12-23T14:37:21Z |
| format | Article |
| id | doaj.art-a626c5766afe4fe08f43527ce59820ea |
| institution | Directory Open Access Journal |
| issn | 1735-7020 2008-238X |
| language | English |
| last_indexed | 2024-12-23T14:37:21Z |
| publishDate | 2016-12-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Parasitology |
| spelling | doaj.art-a626c5766afe4fe08f43527ce59820ea2022-12-21T17:43:18ZengTehran University of Medical SciencesIranian Journal of Parasitology1735-70202008-238X2016-12-01112569Microsporidial Spores in Fecal Samples of Some Domesticated Animals Living in Giza, EgyptAhmad Z. AL-HERRAWY0Mahmoud A. GAD1Dept. of Water Pollution Research, National Research Centre, 12622 Dokki, Giza, EgyptDept. of Water Pollution Research, National Research Centre, 12622 Dokki, Giza, EgyptBackground: The aim of the present work was to investigate the prevalence and species of intestinal microsporidiosis among animals in Giza, Egypt. Methods: A total of 869 animal fecal samples were collected from domesticated animals (dogs, cats, rabbits, cattle, buffaloes, sheep, goats, donkeys and pigs) living in Giza, Egypt. Spores of microsporidia were concentrated from collected samples by centrifugation and finally stained with modified trichrome (MT) stain to detect microsporidial spores. Microsporidial spores in microscopically-positive samples were molecularly confirmed and identified using species-specific primers. Results: Spores of microsporidia were microscopically detected in 17.0% of the examined animal fecal samples. The highest and lowest rates of infection with intestinal microsporidia were recorded in dogs (33.3%) and buffaloes (6.9%), respectively. Molecularly, the obtained microsporidial spores were classified as Enterocytozoon bieneusi and E. intestinalis. Dual infection with both identified species was observed in fecal samples from buffalo, rabbit, goat, cat, pig and dog. Conclusion: Domestic animals may play a role in dissemination of intestinal microsporidiosis in the environment. Examined animals were infected with E. bieneusi in a higher percentage than E. intestinalis.https://ijpa.tums.ac.ir/index.php/ijpa/article/view/1059Intestinal microsporidiaModified trichromePCRDomesticated animalsFecal samples |
| spellingShingle | Ahmad Z. AL-HERRAWY Mahmoud A. GAD Microsporidial Spores in Fecal Samples of Some Domesticated Animals Living in Giza, Egypt Iranian Journal of Parasitology Intestinal microsporidia Modified trichrome PCR Domesticated animals Fecal samples |
| title | Microsporidial Spores in Fecal Samples of Some Domesticated Animals Living in Giza, Egypt |
| title_full | Microsporidial Spores in Fecal Samples of Some Domesticated Animals Living in Giza, Egypt |
| title_fullStr | Microsporidial Spores in Fecal Samples of Some Domesticated Animals Living in Giza, Egypt |
| title_full_unstemmed | Microsporidial Spores in Fecal Samples of Some Domesticated Animals Living in Giza, Egypt |
| title_short | Microsporidial Spores in Fecal Samples of Some Domesticated Animals Living in Giza, Egypt |
| title_sort | microsporidial spores in fecal samples of some domesticated animals living in giza egypt |
| topic | Intestinal microsporidia Modified trichrome PCR Domesticated animals Fecal samples |
| url | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/1059 |
| work_keys_str_mv | AT ahmadzalherrawy microsporidialsporesinfecalsamplesofsomedomesticatedanimalslivingingizaegypt AT mahmoudagad microsporidialsporesinfecalsamplesofsomedomesticatedanimalslivingingizaegypt |