Investigating serumic levels of antibodies against Mycobacterium avium supspecies paratuberculosis using the ELISA method in Holstein dairy cattle
Paratuberculosis (Johne’s disease) is achronic bacterial infection of ruminants causing body wasting due to severe and protracted diarrhea. The aim of the present study was to investigate the rate of infection to Johne’s disease in cattle of Moghan agro-business and animal husbandry complex by detec...
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| Format: | Article |
| Language: | fas |
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Islamic Azad University, Tabriz Branch
2018-08-01
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| Series: | Āsīb/shināsī-i Darmāngāhī-i Dāmpizishkī |
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| Online Access: | http://jvcp.iaut.ac.ir/article_543024_3aeef6b05b1a47c2989846904adcec73.pdf |
| _version_ | 1831757433707429888 |
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| author | yavar heydarzadeh Asiabi Behboud Jafari Ali hassanpour |
| author_facet | yavar heydarzadeh Asiabi Behboud Jafari Ali hassanpour |
| author_sort | yavar heydarzadeh Asiabi |
| collection | DOAJ |
| description | Paratuberculosis (Johne’s disease) is achronic bacterial infection of ruminants causing body wasting due to severe and protracted diarrhea. The aim of the present study was to investigate the rate of infection to Johne’s disease in cattle of Moghan agro-business and animal husbandry complex by detecting antibody titers against <em>Mycobacterium avium</em> subspecies paratuberculosis (MAP) using the absorptive ELISA method. For this purpose, blood samples were collected from 738 randomly selected milking cattle of Moghan agro-business and animal husbandry complex and their sera were assessed by ELISA method. The results indicated that 58 cattle had antibody titers against MAP with S/P percentage over 60% (infection rate of 7.86%) of which 25 cattle were positive (with S/P percentage over 70%) and 33 were suspicious (with S/P percentage between 60-70%). Because Johne’s disease is manifested as a subclinical disease and infected cattle seem to be apparently healthy, bacteria are shed through faeces and body secretions causing infection of the rest of the herd. Thus performing periodic herd screening tests and testing recently introduced animals alongside other preventive measures is strictly advised. |
| first_indexed | 2024-12-22T00:29:51Z |
| format | Article |
| id | doaj.art-a626c9bc8a39486fb5cb6e3e332eb38b |
| institution | Directory Open Access Journal |
| issn | 2322-4746 2476-6984 |
| language | fas |
| last_indexed | 2024-12-22T00:29:51Z |
| publishDate | 2018-08-01 |
| publisher | Islamic Azad University, Tabriz Branch |
| record_format | Article |
| series | Āsīb/shināsī-i Darmāngāhī-i Dāmpizishkī |
| spelling | doaj.art-a626c9bc8a39486fb5cb6e3e332eb38b2022-12-21T18:44:57ZfasIslamic Azad University, Tabriz BranchĀsīb/shināsī-i Darmāngāhī-i Dāmpizishkī2322-47462476-69842018-08-01122 (46) تابستان157166543024Investigating serumic levels of antibodies against Mycobacterium avium supspecies paratuberculosis using the ELISA method in Holstein dairy cattleyavar heydarzadeh Asiabi0Behboud Jafari1Ali hassanpour2Department of Microbiology,Faculty of Basic Science,Ahar,IranDepartment of Microbiology,Faculty of Basic Science,Ahar,IranDepartment of veterinary Medical Science، university of tabriz Azad islami، Tabriz، iranParatuberculosis (Johne’s disease) is achronic bacterial infection of ruminants causing body wasting due to severe and protracted diarrhea. The aim of the present study was to investigate the rate of infection to Johne’s disease in cattle of Moghan agro-business and animal husbandry complex by detecting antibody titers against <em>Mycobacterium avium</em> subspecies paratuberculosis (MAP) using the absorptive ELISA method. For this purpose, blood samples were collected from 738 randomly selected milking cattle of Moghan agro-business and animal husbandry complex and their sera were assessed by ELISA method. The results indicated that 58 cattle had antibody titers against MAP with S/P percentage over 60% (infection rate of 7.86%) of which 25 cattle were positive (with S/P percentage over 70%) and 33 were suspicious (with S/P percentage between 60-70%). Because Johne’s disease is manifested as a subclinical disease and infected cattle seem to be apparently healthy, bacteria are shed through faeces and body secretions causing infection of the rest of the herd. Thus performing periodic herd screening tests and testing recently introduced animals alongside other preventive measures is strictly advised.http://jvcp.iaut.ac.ir/article_543024_3aeef6b05b1a47c2989846904adcec73.pdfMycobacterium avium subspecies paratuberculosisAntibodyELISAdairy cattle |
| spellingShingle | yavar heydarzadeh Asiabi Behboud Jafari Ali hassanpour Investigating serumic levels of antibodies against Mycobacterium avium supspecies paratuberculosis using the ELISA method in Holstein dairy cattle Āsīb/shināsī-i Darmāngāhī-i Dāmpizishkī Mycobacterium avium subspecies paratuberculosis Antibody ELISA dairy cattle |
| title | Investigating serumic levels of antibodies against Mycobacterium avium supspecies paratuberculosis using the ELISA method in Holstein dairy cattle |
| title_full | Investigating serumic levels of antibodies against Mycobacterium avium supspecies paratuberculosis using the ELISA method in Holstein dairy cattle |
| title_fullStr | Investigating serumic levels of antibodies against Mycobacterium avium supspecies paratuberculosis using the ELISA method in Holstein dairy cattle |
| title_full_unstemmed | Investigating serumic levels of antibodies against Mycobacterium avium supspecies paratuberculosis using the ELISA method in Holstein dairy cattle |
| title_short | Investigating serumic levels of antibodies against Mycobacterium avium supspecies paratuberculosis using the ELISA method in Holstein dairy cattle |
| title_sort | investigating serumic levels of antibodies against mycobacterium avium supspecies paratuberculosis using the elisa method in holstein dairy cattle |
| topic | Mycobacterium avium subspecies paratuberculosis Antibody ELISA dairy cattle |
| url | http://jvcp.iaut.ac.ir/article_543024_3aeef6b05b1a47c2989846904adcec73.pdf |
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