GABA Immunoreactivity and Pharmacological Effects vary Among Stylet-Bearing Nematodes
Plant-parasitic nematodes conduct a series of sophisticated behaviors to complete their life cycles. Among these, locomotion behaviors, including finding the host and migrating to the feeding site, directly affect the success of parasitism. Thus, disrupting locomotion behaviors has the potential to...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Sciendo
2023-11-01
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| Series: | Journal of Nematology |
| Subjects: | |
| Online Access: | https://doi.org/10.2478/jofnem-2023-0049 |
| _version_ | 1797219133336584192 |
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| author | Reed Hannah M. Han Ziduan Schroeder Nathan E. |
| author_facet | Reed Hannah M. Han Ziduan Schroeder Nathan E. |
| author_sort | Reed Hannah M. |
| collection | DOAJ |
| description | Plant-parasitic nematodes conduct a series of sophisticated behaviors to complete their life cycles. Among these, locomotion behaviors, including finding the host and migrating to the feeding site, directly affect the success of parasitism. Thus, disrupting locomotion behaviors has the potential to control these parasites. γ-Aminobutyric acid (GABA) is the prominent inhibitory neurotransmitter in nematodes. GABA-immunoreactive neurons are mostly found in motor neurons, where they regulate behaviors in the model nematode C. elegans. However, the GABA system in most stylet-bearing nematodes has received little attention. Using immunohistochemistry, we found variation in the pattern of GABA-immunoreactivity among two major plant-parasites and a fungal feeder. Some of these GABA-immunoreactive neurons lack clear homologs to C. elegans. Pharmaceutical assays showed that applying GABA, its agonist, and its antagonist, can disrupt the locomotion behaviors of these nematodes, although sensitivity to a given compound varied between species. Our data suggest that the GABA system is a potential target for the control of plant-parasitic nematodes. |
| first_indexed | 2024-03-08T10:02:57Z |
| format | Article |
| id | doaj.art-a6285e74ad83496ebb4e9a8e43aa1249 |
| institution | Directory Open Access Journal |
| issn | 2640-396X |
| language | English |
| last_indexed | 2024-04-24T12:28:48Z |
| publishDate | 2023-11-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Journal of Nematology |
| spelling | doaj.art-a6285e74ad83496ebb4e9a8e43aa12492024-04-08T07:37:21ZengSciendoJournal of Nematology2640-396X2023-11-015517233610.2478/jofnem-2023-0049GABA Immunoreactivity and Pharmacological Effects vary Among Stylet-Bearing NematodesReed Hannah M.0Han Ziduan1Schroeder Nathan E.2Department of Crop Sciences, University of Illinois at Urbana-Champaign, ILDepartment of Crop Sciences, University of Illinois at Urbana-Champaign, ILDepartment of Crop Sciences, University of Illinois at Urbana-Champaign, ILPlant-parasitic nematodes conduct a series of sophisticated behaviors to complete their life cycles. Among these, locomotion behaviors, including finding the host and migrating to the feeding site, directly affect the success of parasitism. Thus, disrupting locomotion behaviors has the potential to control these parasites. γ-Aminobutyric acid (GABA) is the prominent inhibitory neurotransmitter in nematodes. GABA-immunoreactive neurons are mostly found in motor neurons, where they regulate behaviors in the model nematode C. elegans. However, the GABA system in most stylet-bearing nematodes has received little attention. Using immunohistochemistry, we found variation in the pattern of GABA-immunoreactivity among two major plant-parasites and a fungal feeder. Some of these GABA-immunoreactive neurons lack clear homologs to C. elegans. Pharmaceutical assays showed that applying GABA, its agonist, and its antagonist, can disrupt the locomotion behaviors of these nematodes, although sensitivity to a given compound varied between species. Our data suggest that the GABA system is a potential target for the control of plant-parasitic nematodes.https://doi.org/10.2478/jofnem-2023-0049antibody stainingbehaviornematode controlneural anatomyplant-parasitic nematode |
| spellingShingle | Reed Hannah M. Han Ziduan Schroeder Nathan E. GABA Immunoreactivity and Pharmacological Effects vary Among Stylet-Bearing Nematodes Journal of Nematology antibody staining behavior nematode control neural anatomy plant-parasitic nematode |
| title | GABA Immunoreactivity and Pharmacological Effects vary Among Stylet-Bearing Nematodes |
| title_full | GABA Immunoreactivity and Pharmacological Effects vary Among Stylet-Bearing Nematodes |
| title_fullStr | GABA Immunoreactivity and Pharmacological Effects vary Among Stylet-Bearing Nematodes |
| title_full_unstemmed | GABA Immunoreactivity and Pharmacological Effects vary Among Stylet-Bearing Nematodes |
| title_short | GABA Immunoreactivity and Pharmacological Effects vary Among Stylet-Bearing Nematodes |
| title_sort | gaba immunoreactivity and pharmacological effects vary among stylet bearing nematodes |
| topic | antibody staining behavior nematode control neural anatomy plant-parasitic nematode |
| url | https://doi.org/10.2478/jofnem-2023-0049 |
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