Integrating transcriptomics and metabolomics to analyze quinoa (Chenopodium quinoa Willd.) responses to drought stress and rewatering
The crop production of quinoa (Chenopodium quinoa Willd.), the only plant meeting basic human nutritional requirements, is affected by drought stress. To better understand the drought tolerance mechanism of quinoa, we screened the drought-tolerant quinoa genotype “Dianli 129” and studied the seedlin...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.988861/full |
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| author | Xiuju Huan Li Li Yongjiang Liu Zhiyou Kong Yeju Liu Qianchao Wang Junna Liu Ping Zhang Yirui Guo Peng Qin |
| author_facet | Xiuju Huan Li Li Yongjiang Liu Zhiyou Kong Yeju Liu Qianchao Wang Junna Liu Ping Zhang Yirui Guo Peng Qin |
| author_sort | Xiuju Huan |
| collection | DOAJ |
| description | The crop production of quinoa (Chenopodium quinoa Willd.), the only plant meeting basic human nutritional requirements, is affected by drought stress. To better understand the drought tolerance mechanism of quinoa, we screened the drought-tolerant quinoa genotype “Dianli 129” and studied the seedling leaves of the drought-tolerant quinoa genotype after drought and rewatering treatments using transcriptomics and targeted metabolomics. Drought-treatment, drought control, rewatering-treated, and rewatered control were named as DR, DC, RW, and RC, respectively. Among four comparison groups, DC vs. DR, RC vs. RW, RW vs. DR, and RC vs. DC, we identified 10,292, 2,307, 12,368, and 3 differentially expressed genes (DEGs), and 215, 192, 132, and 19 differentially expressed metabolites (DEMs), respectively. A total of 38,670 genes and 142 pathways were annotated. The results of transcriptome and metabolome association analysis showed that gene-LOC110713661 and gene-LOC110738152 may be the key genes for drought tolerance in quinoa. Some metabolites accumulated in quinoa leaves in response to drought stress, and the plants recovered after rewatering. DEGs and DEMs participate in starch and sucrose metabolism and flavonoid biosynthesis, which are vital for improving drought tolerance in quinoa. Drought tolerance of quinoa was correlated with gene expression differences, metabolite accumulation and good recovery after rewatering. These findings improve our understanding of drought and rewatering responses in quinoa and have implications for the breeding of new drought-tolerance varieties while providing a theoretical basis for drought-tolerance varieties identification. |
| first_indexed | 2024-04-11T08:28:08Z |
| format | Article |
| id | doaj.art-a6296122d2254ce184220848d726daea |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-11T08:28:08Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-a6296122d2254ce184220848d726daea2022-12-22T04:34:38ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-10-011310.3389/fpls.2022.988861988861Integrating transcriptomics and metabolomics to analyze quinoa (Chenopodium quinoa Willd.) responses to drought stress and rewateringXiuju Huan0Li Li1Yongjiang Liu2Zhiyou Kong3Yeju Liu4Qianchao Wang5Junna Liu6Ping Zhang7Yirui Guo8Peng Qin9College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, ChinaCollege of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, ChinaCollege of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, ChinaCollege of Resources and Environment, Baoshan College, Baoshan, ChinaGraduate Office, Yunnan Agricultural University, Kunming, ChinaCollege of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, ChinaCollege of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, ChinaCollege of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, ChinaCollege of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, ChinaCollege of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, ChinaThe crop production of quinoa (Chenopodium quinoa Willd.), the only plant meeting basic human nutritional requirements, is affected by drought stress. To better understand the drought tolerance mechanism of quinoa, we screened the drought-tolerant quinoa genotype “Dianli 129” and studied the seedling leaves of the drought-tolerant quinoa genotype after drought and rewatering treatments using transcriptomics and targeted metabolomics. Drought-treatment, drought control, rewatering-treated, and rewatered control were named as DR, DC, RW, and RC, respectively. Among four comparison groups, DC vs. DR, RC vs. RW, RW vs. DR, and RC vs. DC, we identified 10,292, 2,307, 12,368, and 3 differentially expressed genes (DEGs), and 215, 192, 132, and 19 differentially expressed metabolites (DEMs), respectively. A total of 38,670 genes and 142 pathways were annotated. The results of transcriptome and metabolome association analysis showed that gene-LOC110713661 and gene-LOC110738152 may be the key genes for drought tolerance in quinoa. Some metabolites accumulated in quinoa leaves in response to drought stress, and the plants recovered after rewatering. DEGs and DEMs participate in starch and sucrose metabolism and flavonoid biosynthesis, which are vital for improving drought tolerance in quinoa. Drought tolerance of quinoa was correlated with gene expression differences, metabolite accumulation and good recovery after rewatering. These findings improve our understanding of drought and rewatering responses in quinoa and have implications for the breeding of new drought-tolerance varieties while providing a theoretical basis for drought-tolerance varieties identification.https://www.frontiersin.org/articles/10.3389/fpls.2022.988861/fullquinoadrought stresstranscriptomicsmetabolomicsflavonoid biosynthesis |
| spellingShingle | Xiuju Huan Li Li Yongjiang Liu Zhiyou Kong Yeju Liu Qianchao Wang Junna Liu Ping Zhang Yirui Guo Peng Qin Integrating transcriptomics and metabolomics to analyze quinoa (Chenopodium quinoa Willd.) responses to drought stress and rewatering Frontiers in Plant Science quinoa drought stress transcriptomics metabolomics flavonoid biosynthesis |
| title | Integrating transcriptomics and metabolomics to analyze quinoa (Chenopodium quinoa Willd.) responses to drought stress and rewatering |
| title_full | Integrating transcriptomics and metabolomics to analyze quinoa (Chenopodium quinoa Willd.) responses to drought stress and rewatering |
| title_fullStr | Integrating transcriptomics and metabolomics to analyze quinoa (Chenopodium quinoa Willd.) responses to drought stress and rewatering |
| title_full_unstemmed | Integrating transcriptomics and metabolomics to analyze quinoa (Chenopodium quinoa Willd.) responses to drought stress and rewatering |
| title_short | Integrating transcriptomics and metabolomics to analyze quinoa (Chenopodium quinoa Willd.) responses to drought stress and rewatering |
| title_sort | integrating transcriptomics and metabolomics to analyze quinoa chenopodium quinoa willd responses to drought stress and rewatering |
| topic | quinoa drought stress transcriptomics metabolomics flavonoid biosynthesis |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.988861/full |
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