Transcriptome Analysis Identifies Strategies Targeting Immune Response-Related Pathways to Control Enterotoxigenic Escherichia coli Infection in Porcine Intestinal Epithelial Cells
Enterotoxigenic Escherichia coli (ETEC) is an important cause of post-weaning diarrhea (PWD) worldwide, resulting in huge economic losses to the swine industry worldwide. In this study, to understand the pathogenesis, the transcriptomic analysis was performed to explore the biological processes (BP)...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-08-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2021.677897/full |
| _version_ | 1819146057656827904 |
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| author | Qiong Wu Qiong Wu Defeng Cui Defeng Cui Xinyu Chao Peng Chen Jiaxuan Liu Yiding Wang Tongjian Su Meng Li Ruyu Xu Yaohong Zhu Yonghong Zhang Yonghong Zhang |
| author_facet | Qiong Wu Qiong Wu Defeng Cui Defeng Cui Xinyu Chao Peng Chen Jiaxuan Liu Yiding Wang Tongjian Su Meng Li Ruyu Xu Yaohong Zhu Yonghong Zhang Yonghong Zhang |
| author_sort | Qiong Wu |
| collection | DOAJ |
| description | Enterotoxigenic Escherichia coli (ETEC) is an important cause of post-weaning diarrhea (PWD) worldwide, resulting in huge economic losses to the swine industry worldwide. In this study, to understand the pathogenesis, the transcriptomic analysis was performed to explore the biological processes (BP) in porcine intestinal epithelial J2 cells infected with an emerging ETEC strain isolated from weaned pigs with diarrhea. Under the criteria of |fold change| (FC) ≥ 2 and P < 0.05 with false discovery rate < 0.05, a total of 131 referenced and 19 novel differentially expressed genes (DEGs) were identified after ETEC infection, including 96 upregulated DEGs and 54 downregulated DEGs. The Gene Ontology (GO) analysis of DEGs showed that ETEC evoked BP specifically involved in response to lipopolysaccharide (LPS) and negative regulation of intracellular signal transduction. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that immune response-related pathways were mainly enriched in J2 cells after ETEC infection, in which tumor necrosis factor (TNF), interleukin 17, and mitogen-activated protein kinase (MAPK) signaling pathways possessed the highest rich factor, followed by nucleotide-binding and oligomerization domain-like receptor (NLRs), C-type lectin receptor (CLR), cytokine–cytokine receptor interaction, and Toll-like receptor (TLR), and nuclear factor kappa-B (NF-κB) signaling pathways. Furthermore, 30 of 131 referenced DEGs, especially the nuclear transcription factor AP-1 and NF-κB, participate in the immune response to infection through an integral signal cascade and can be target molecules for prevention and control of enteric ETEC infection by probiotic Lactobacillus reuteri. Our data provide a comprehensive insight into the immune response of porcine intestinal epithelial cells (IECs) to ETEC infection and advance the identification of targets for prevention and control of ETEC-related PWD. |
| first_indexed | 2024-12-22T13:07:52Z |
| format | Article |
| id | doaj.art-a62cafccf7334fc68a1f21620149d4eb |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-12-22T13:07:52Z |
| publishDate | 2021-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-a62cafccf7334fc68a1f21620149d4eb2022-12-21T18:24:49ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692021-08-01810.3389/fvets.2021.677897677897Transcriptome Analysis Identifies Strategies Targeting Immune Response-Related Pathways to Control Enterotoxigenic Escherichia coli Infection in Porcine Intestinal Epithelial CellsQiong Wu0Qiong Wu1Defeng Cui2Defeng Cui3Xinyu Chao4Peng Chen5Jiaxuan Liu6Yiding Wang7Tongjian Su8Meng Li9Ruyu Xu10Yaohong Zhu11Yonghong Zhang12Yonghong Zhang13Department of Animal Medicine, College of Animal Science and Technology, Beijing University of Agriculture, Beijing, ChinaBeijing Key Laboratory of Traditional Chinese Veterinary Medicine, Beijing University of Agriculture, Beijing, ChinaDepartment of Animal Medicine, College of Animal Science and Technology, Beijing University of Agriculture, Beijing, ChinaBeijing Key Laboratory of Traditional Chinese Veterinary Medicine, Beijing University of Agriculture, Beijing, ChinaDepartment of Animal Medicine, College of Animal Science and Technology, Beijing University of Agriculture, Beijing, ChinaDepartment of Animal Medicine, College of Animal Science and Technology, Beijing University of Agriculture, Beijing, ChinaDepartment of Animal Medicine, College of Animal Science and Technology, Beijing University of Agriculture, Beijing, ChinaDepartment of Animal Medicine, College of Animal Science and Technology, Beijing University of Agriculture, Beijing, ChinaDepartment of Animal Medicine, College of Animal Science and Technology, Beijing University of Agriculture, Beijing, ChinaDepartment of Animal Medicine, College of Animal Science and Technology, Beijing University of Agriculture, Beijing, ChinaDepartment of Animal Medicine, College of Animal Science and Technology, Beijing University of Agriculture, Beijing, ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, China Agricultural University, Beijing, ChinaDepartment of Animal Medicine, College of Animal Science and Technology, Beijing University of Agriculture, Beijing, ChinaBeijing Key Laboratory of Traditional Chinese Veterinary Medicine, Beijing University of Agriculture, Beijing, ChinaEnterotoxigenic Escherichia coli (ETEC) is an important cause of post-weaning diarrhea (PWD) worldwide, resulting in huge economic losses to the swine industry worldwide. In this study, to understand the pathogenesis, the transcriptomic analysis was performed to explore the biological processes (BP) in porcine intestinal epithelial J2 cells infected with an emerging ETEC strain isolated from weaned pigs with diarrhea. Under the criteria of |fold change| (FC) ≥ 2 and P < 0.05 with false discovery rate < 0.05, a total of 131 referenced and 19 novel differentially expressed genes (DEGs) were identified after ETEC infection, including 96 upregulated DEGs and 54 downregulated DEGs. The Gene Ontology (GO) analysis of DEGs showed that ETEC evoked BP specifically involved in response to lipopolysaccharide (LPS) and negative regulation of intracellular signal transduction. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that immune response-related pathways were mainly enriched in J2 cells after ETEC infection, in which tumor necrosis factor (TNF), interleukin 17, and mitogen-activated protein kinase (MAPK) signaling pathways possessed the highest rich factor, followed by nucleotide-binding and oligomerization domain-like receptor (NLRs), C-type lectin receptor (CLR), cytokine–cytokine receptor interaction, and Toll-like receptor (TLR), and nuclear factor kappa-B (NF-κB) signaling pathways. Furthermore, 30 of 131 referenced DEGs, especially the nuclear transcription factor AP-1 and NF-κB, participate in the immune response to infection through an integral signal cascade and can be target molecules for prevention and control of enteric ETEC infection by probiotic Lactobacillus reuteri. Our data provide a comprehensive insight into the immune response of porcine intestinal epithelial cells (IECs) to ETEC infection and advance the identification of targets for prevention and control of ETEC-related PWD.https://www.frontiersin.org/articles/10.3389/fvets.2021.677897/fulltranscriptome (RNA-seq)intestinal epithelia cellimmune responseEscherichia coliporcineenrichment analysis |
| spellingShingle | Qiong Wu Qiong Wu Defeng Cui Defeng Cui Xinyu Chao Peng Chen Jiaxuan Liu Yiding Wang Tongjian Su Meng Li Ruyu Xu Yaohong Zhu Yonghong Zhang Yonghong Zhang Transcriptome Analysis Identifies Strategies Targeting Immune Response-Related Pathways to Control Enterotoxigenic Escherichia coli Infection in Porcine Intestinal Epithelial Cells Frontiers in Veterinary Science transcriptome (RNA-seq) intestinal epithelia cell immune response Escherichia coli porcine enrichment analysis |
| title | Transcriptome Analysis Identifies Strategies Targeting Immune Response-Related Pathways to Control Enterotoxigenic Escherichia coli Infection in Porcine Intestinal Epithelial Cells |
| title_full | Transcriptome Analysis Identifies Strategies Targeting Immune Response-Related Pathways to Control Enterotoxigenic Escherichia coli Infection in Porcine Intestinal Epithelial Cells |
| title_fullStr | Transcriptome Analysis Identifies Strategies Targeting Immune Response-Related Pathways to Control Enterotoxigenic Escherichia coli Infection in Porcine Intestinal Epithelial Cells |
| title_full_unstemmed | Transcriptome Analysis Identifies Strategies Targeting Immune Response-Related Pathways to Control Enterotoxigenic Escherichia coli Infection in Porcine Intestinal Epithelial Cells |
| title_short | Transcriptome Analysis Identifies Strategies Targeting Immune Response-Related Pathways to Control Enterotoxigenic Escherichia coli Infection in Porcine Intestinal Epithelial Cells |
| title_sort | transcriptome analysis identifies strategies targeting immune response related pathways to control enterotoxigenic escherichia coli infection in porcine intestinal epithelial cells |
| topic | transcriptome (RNA-seq) intestinal epithelia cell immune response Escherichia coli porcine enrichment analysis |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2021.677897/full |
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