Isolation of three different sizes of exosomes in an Asian population with different retinal diseases before and after treatment: preliminary results
ABSTRACTExosomes are membranous structures measuring between 40–120 nm that are secreted by various cells of the human body into the body fluid system. Exosomes contain proteins, mRNA, miRNA, and signaling molecules, and physiologically they assist in the intercellular transport of proteins and RNA...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Bioengineered |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21655979.2023.2297320 |
| _version_ | 1797353549117521920 |
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| author | Sung-Yu Wu Yu-Chien Hung Chien-Chih Chou Connie Chen Chao-Min Cheng Chihchen Chen Jyh-Cheng Liou Min-Yen Hsu |
| author_facet | Sung-Yu Wu Yu-Chien Hung Chien-Chih Chou Connie Chen Chao-Min Cheng Chihchen Chen Jyh-Cheng Liou Min-Yen Hsu |
| author_sort | Sung-Yu Wu |
| collection | DOAJ |
| description | ABSTRACTExosomes are membranous structures measuring between 40–120 nm that are secreted by various cells of the human body into the body fluid system. Exosomes contain proteins, mRNA, miRNA, and signaling molecules, and physiologically they assist in the intercellular transport of proteins and RNA molecules. In this study, we used an immunoaffinity filter paper platform combined with scanning electron microscopy and microfluidic systems to detect the size of exosomes within the aqueous humor. Eight aqueous humor samples showed three distinct sizes of exosomes that were significantly different on scanning electron microscopy(P < 0.01). We further used nanoparticle tracking analysis to assess the size distribution of exosomes within the aqueous humor. We found significantly different distributions of exosomes between patients with three different ocular diseases and patients with normal cataracts as controls. An obvious peak of exomeres(size around 35 nm)was found in the patients with central retinal vein occlusion and vitreous hemorrhage. Flare-ups of large exosomes(size 90–120 nm)were found in the patients with the inflammatory ocular disease pars planitis. No obvious peaks in exomeres or large exosomes were found in the control group. There was a high association between the distribution of exosomes and the pathogenesis of ocular diseases. After intravitreal anti-vascular endothelial growth factor treatment, the aqueous humor from the patients with neovascular diseases showed a significant reduction in exosomes in nanoparticle tracking analysis. These findings suggest that at least three distinct sizes of exosomes exist in the aqueous humor:(1)exomeres:<35 nm;(2)small exosomes:60–80 nm; and (3)large exosomes:90–120 nm. Different sizes of exosomes may have different implications in normal or diseased eyes. |
| first_indexed | 2024-03-08T13:32:40Z |
| format | Article |
| id | doaj.art-a62cb117b34f486492d380b4c7033042 |
| institution | Directory Open Access Journal |
| issn | 2165-5979 2165-5987 |
| language | English |
| last_indexed | 2024-03-08T13:32:40Z |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Bioengineered |
| spelling | doaj.art-a62cb117b34f486492d380b4c70330422024-01-17T05:04:34ZengTaylor & Francis GroupBioengineered2165-59792165-59872024-12-0115110.1080/21655979.2023.2297320Isolation of three different sizes of exosomes in an Asian population with different retinal diseases before and after treatment: preliminary resultsSung-Yu Wu0Yu-Chien Hung1Chien-Chih Chou2Connie Chen3Chao-Min Cheng4Chihchen Chen5Jyh-Cheng Liou6Min-Yen Hsu7School of Medicine, Chung Shan Medical University, Taichung, TaiwanSchool of Medicine, Chung Shan Medical University, Taichung, TaiwanGraduate Institute of Clinical Medicine, College of Medicine, National Taiwan University, Taipei, TaiwanDepartment of Ophthalmology, Chung Shan Medical University Hospital, Taichung, TaiwanInstitute of Biomedical Engineering, National Tsing Hua University, Hsinchu, TaiwanInstitute of Nanoengineering and Microsystem, National Tsing Hua University, Hsinchu, TaiwanSchool of Medicine, Chung Shan Medical University, Taichung, TaiwanSchool of Medicine, Chung Shan Medical University, Taichung, TaiwanABSTRACTExosomes are membranous structures measuring between 40–120 nm that are secreted by various cells of the human body into the body fluid system. Exosomes contain proteins, mRNA, miRNA, and signaling molecules, and physiologically they assist in the intercellular transport of proteins and RNA molecules. In this study, we used an immunoaffinity filter paper platform combined with scanning electron microscopy and microfluidic systems to detect the size of exosomes within the aqueous humor. Eight aqueous humor samples showed three distinct sizes of exosomes that were significantly different on scanning electron microscopy(P < 0.01). We further used nanoparticle tracking analysis to assess the size distribution of exosomes within the aqueous humor. We found significantly different distributions of exosomes between patients with three different ocular diseases and patients with normal cataracts as controls. An obvious peak of exomeres(size around 35 nm)was found in the patients with central retinal vein occlusion and vitreous hemorrhage. Flare-ups of large exosomes(size 90–120 nm)were found in the patients with the inflammatory ocular disease pars planitis. No obvious peaks in exomeres or large exosomes were found in the control group. There was a high association between the distribution of exosomes and the pathogenesis of ocular diseases. After intravitreal anti-vascular endothelial growth factor treatment, the aqueous humor from the patients with neovascular diseases showed a significant reduction in exosomes in nanoparticle tracking analysis. These findings suggest that at least three distinct sizes of exosomes exist in the aqueous humor:(1)exomeres:<35 nm;(2)small exosomes:60–80 nm; and (3)large exosomes:90–120 nm. Different sizes of exosomes may have different implications in normal or diseased eyes.https://www.tandfonline.com/doi/10.1080/21655979.2023.2297320Exosomeaqueous humornanoparticle tracking analysisocular diseaseexomere |
| spellingShingle | Sung-Yu Wu Yu-Chien Hung Chien-Chih Chou Connie Chen Chao-Min Cheng Chihchen Chen Jyh-Cheng Liou Min-Yen Hsu Isolation of three different sizes of exosomes in an Asian population with different retinal diseases before and after treatment: preliminary results Bioengineered Exosome aqueous humor nanoparticle tracking analysis ocular disease exomere |
| title | Isolation of three different sizes of exosomes in an Asian population with different retinal diseases before and after treatment: preliminary results |
| title_full | Isolation of three different sizes of exosomes in an Asian population with different retinal diseases before and after treatment: preliminary results |
| title_fullStr | Isolation of three different sizes of exosomes in an Asian population with different retinal diseases before and after treatment: preliminary results |
| title_full_unstemmed | Isolation of three different sizes of exosomes in an Asian population with different retinal diseases before and after treatment: preliminary results |
| title_short | Isolation of three different sizes of exosomes in an Asian population with different retinal diseases before and after treatment: preliminary results |
| title_sort | isolation of three different sizes of exosomes in an asian population with different retinal diseases before and after treatment preliminary results |
| topic | Exosome aqueous humor nanoparticle tracking analysis ocular disease exomere |
| url | https://www.tandfonline.com/doi/10.1080/21655979.2023.2297320 |
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