In Vitro Antioxidant Activity and In Vivo Anti-Fatigue Effect of Sea Horse (Hippocampus) Peptides
This study investigated changes the in vitro antioxidant activity of Hippocampus polypeptides during enzymatic hydrolysis, including the effects of enzyme species, enzyme concentration, material–liquid ratio, hydrolysis time, pH, and temperature of the reaction system. Its in vivo anti-fatigue activ...
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MDPI AG
2017-03-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/22/3/482 |
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| author | Zebin Guo Duanquan Lin Juanjuan Guo Yi Zhang Baodong Zheng |
| author_facet | Zebin Guo Duanquan Lin Juanjuan Guo Yi Zhang Baodong Zheng |
| author_sort | Zebin Guo |
| collection | DOAJ |
| description | This study investigated changes the in vitro antioxidant activity of Hippocampus polypeptides during enzymatic hydrolysis, including the effects of enzyme species, enzyme concentration, material–liquid ratio, hydrolysis time, pH, and temperature of the reaction system. Its in vivo anti-fatigue activity was also studied. Hippocampus peptide prepared by papain digestion exhibited the highest 1,1-diphenyl-2-picryl-hydrazyl free radical scavenging rate (71.89% ± 1.50%) and strong hydroxyl radical scavenging rate (75.53% ± 0.98%), compared to those prepared by five other commonly used enzymes (i.e., trypsin, neutral protease, compound protease, flavorzyme, and alkaline protease). Additionally, maximum antioxidant activity of Hippocampus polypeptide prepared by papain digestion was reached after hydrolysis for 40 min at pH 6.0 and 60 °C of the reaction system by using 2000 U/g enzyme and a material–liquid ratio of 1:15. Moreover, compared with the control group, Hippocampus peptide prolonged the swimming time by 33%–40%, stabilized the blood glucose concentration, increased liver glycogen levels, and decreased blood lactate levels and blood urea nitrogen levels in mice (p < 0.01). In conclusion, these results indicated that Hippocampus polypeptide prepared by papain digestion under optimal conditions exhibited high degrees of antioxidant and anti-fatigue activity. |
| first_indexed | 2024-12-13T02:05:49Z |
| format | Article |
| id | doaj.art-a62dd3398dfd457aa0774839bf33d4b7 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-13T02:05:49Z |
| publishDate | 2017-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-a62dd3398dfd457aa0774839bf33d4b72022-12-22T00:03:08ZengMDPI AGMolecules1420-30492017-03-0122348210.3390/molecules22030482molecules22030482In Vitro Antioxidant Activity and In Vivo Anti-Fatigue Effect of Sea Horse (Hippocampus) PeptidesZebin Guo0Duanquan Lin1Juanjuan Guo2Yi Zhang3Baodong Zheng4College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350000, Fujian, ChinaCollege of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350000, Fujian, ChinaCollege of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350000, Fujian, ChinaCollege of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350000, Fujian, ChinaCollege of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350000, Fujian, ChinaThis study investigated changes the in vitro antioxidant activity of Hippocampus polypeptides during enzymatic hydrolysis, including the effects of enzyme species, enzyme concentration, material–liquid ratio, hydrolysis time, pH, and temperature of the reaction system. Its in vivo anti-fatigue activity was also studied. Hippocampus peptide prepared by papain digestion exhibited the highest 1,1-diphenyl-2-picryl-hydrazyl free radical scavenging rate (71.89% ± 1.50%) and strong hydroxyl radical scavenging rate (75.53% ± 0.98%), compared to those prepared by five other commonly used enzymes (i.e., trypsin, neutral protease, compound protease, flavorzyme, and alkaline protease). Additionally, maximum antioxidant activity of Hippocampus polypeptide prepared by papain digestion was reached after hydrolysis for 40 min at pH 6.0 and 60 °C of the reaction system by using 2000 U/g enzyme and a material–liquid ratio of 1:15. Moreover, compared with the control group, Hippocampus peptide prolonged the swimming time by 33%–40%, stabilized the blood glucose concentration, increased liver glycogen levels, and decreased blood lactate levels and blood urea nitrogen levels in mice (p < 0.01). In conclusion, these results indicated that Hippocampus polypeptide prepared by papain digestion under optimal conditions exhibited high degrees of antioxidant and anti-fatigue activity.http://www.mdpi.com/1420-3049/22/3/482peptideHippocampusantioxidant activityanti-fatigue effect |
| spellingShingle | Zebin Guo Duanquan Lin Juanjuan Guo Yi Zhang Baodong Zheng In Vitro Antioxidant Activity and In Vivo Anti-Fatigue Effect of Sea Horse (Hippocampus) Peptides Molecules peptide Hippocampus antioxidant activity anti-fatigue effect |
| title | In Vitro Antioxidant Activity and In Vivo Anti-Fatigue Effect of Sea Horse (Hippocampus) Peptides |
| title_full | In Vitro Antioxidant Activity and In Vivo Anti-Fatigue Effect of Sea Horse (Hippocampus) Peptides |
| title_fullStr | In Vitro Antioxidant Activity and In Vivo Anti-Fatigue Effect of Sea Horse (Hippocampus) Peptides |
| title_full_unstemmed | In Vitro Antioxidant Activity and In Vivo Anti-Fatigue Effect of Sea Horse (Hippocampus) Peptides |
| title_short | In Vitro Antioxidant Activity and In Vivo Anti-Fatigue Effect of Sea Horse (Hippocampus) Peptides |
| title_sort | in vitro antioxidant activity and in vivo anti fatigue effect of sea horse hippocampus peptides |
| topic | peptide Hippocampus antioxidant activity anti-fatigue effect |
| url | http://www.mdpi.com/1420-3049/22/3/482 |
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