Recombination Located over 2A-2B Junction Ribosome Frameshifting Region of Saffold Cardiovirus
Here we report the nearly full-length genome of a recombinant Saffold virus strain (SAFV-BR-193) isolated from a child with acute gastroenteritis. Evolutionary analysis performed using all available near-full length Saffold picornavirus genomes showed that the breakpoint found in the Brazilian strai...
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-09-01
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| Series: | Viruses |
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| Online Access: | http://www.mdpi.com/1999-4915/10/10/520 |
| _version_ | 1819032750831697920 |
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| author | Antônio Charlys da Costa Adriana Luchs Flávio Augusto de Pádua Milagres Shirley Vasconcelos Komninakis Danielle Elise Gill Márcia Cristina Alves Brito Sayão Lobato Rafael Brustulin Rogério Togisaki das Chagas Maria de Fátima Neves dos Santos Abrão Cassia Vitória de Deus Alves Soares Xutao Deng Ester Cerdeira Sabino Eric Delwart Élcio Leal |
| author_facet | Antônio Charlys da Costa Adriana Luchs Flávio Augusto de Pádua Milagres Shirley Vasconcelos Komninakis Danielle Elise Gill Márcia Cristina Alves Brito Sayão Lobato Rafael Brustulin Rogério Togisaki das Chagas Maria de Fátima Neves dos Santos Abrão Cassia Vitória de Deus Alves Soares Xutao Deng Ester Cerdeira Sabino Eric Delwart Élcio Leal |
| author_sort | Antônio Charlys da Costa |
| collection | DOAJ |
| description | Here we report the nearly full-length genome of a recombinant Saffold virus strain (SAFV-BR-193) isolated from a child with acute gastroenteritis. Evolutionary analysis performed using all available near-full length Saffold picornavirus genomes showed that the breakpoint found in the Brazilian strain (SAFV-BR-193) is indeed a recombination hotspot. Notably, this hotspot is located just one nucleotide after the ribosomal frameshift GGUUUUU motif in the SAFV genome. Empirical studies will be necessary to determine if this motif also affects the binding affinity of RNA-dependent RNA-polymerase (RdRp) and therefore increases the changes of RdRp swap between molecules during the synthesis of viral genomes. |
| first_indexed | 2024-12-21T07:06:55Z |
| format | Article |
| id | doaj.art-a62f66ccd7854698a9cb0b25431419c0 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-12-21T07:06:55Z |
| publishDate | 2018-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-a62f66ccd7854698a9cb0b25431419c02022-12-21T19:12:04ZengMDPI AGViruses1999-49152018-09-01101052010.3390/v10100520v10100520Recombination Located over 2A-2B Junction Ribosome Frameshifting Region of Saffold CardiovirusAntônio Charlys da Costa0Adriana Luchs1Flávio Augusto de Pádua Milagres2Shirley Vasconcelos Komninakis3Danielle Elise Gill4Márcia Cristina Alves Brito Sayão Lobato5Rafael Brustulin6Rogério Togisaki das Chagas7Maria de Fátima Neves dos Santos Abrão8Cassia Vitória de Deus Alves Soares9Xutao Deng10Ester Cerdeira Sabino11Eric Delwart12Élcio Leal13Institute of Tropical Medicine, University of São Paulo, São Paulo 05403-000, BrazilEnteric Disease Laboratory, Virology Center, Adolfo Lutz Institute, São Paulo 01246-000, BrazilLIM/46, Faculty of Medicine, University of São Paulo, São Paulo 01246-903, BrazilPostgraduate Program in Health Science, Faculty of Medicine of ABC, Santo André 09060-870, BrazilInstitute of Tropical Medicine, University of São Paulo, São Paulo 05403-000, BrazilSecretary of Health of Tocantins, Tocantins 77453-000, BrazilSecretary of Health of Tocantins, Tocantins 77453-000, BrazilSecretary of Health of Tocantins, Tocantins 77453-000, BrazilSecretary of Health of Tocantins, Tocantins 77453-000, BrazilSecretary of Health of Tocantins, Tocantins 77453-000, BrazilBlood Systems Research Institute, San Francisco, CA 94143, USAInstitute of Tropical Medicine, University of São Paulo, São Paulo 05403-000, BrazilBlood Systems Research Institute, San Francisco, CA 94143, USAInstitute of Biological Sciences, Federal University of Pará, Pará 66075-000, BrazilHere we report the nearly full-length genome of a recombinant Saffold virus strain (SAFV-BR-193) isolated from a child with acute gastroenteritis. Evolutionary analysis performed using all available near-full length Saffold picornavirus genomes showed that the breakpoint found in the Brazilian strain (SAFV-BR-193) is indeed a recombination hotspot. Notably, this hotspot is located just one nucleotide after the ribosomal frameshift GGUUUUU motif in the SAFV genome. Empirical studies will be necessary to determine if this motif also affects the binding affinity of RNA-dependent RNA-polymerase (RdRp) and therefore increases the changes of RdRp swap between molecules during the synthesis of viral genomes.http://www.mdpi.com/1999-4915/10/10/520saffold viruscardiovirusviromepicornavirusribosomal frameshiftingGGUUUUU motifRNA-dependent RNA-polymerase |
| spellingShingle | Antônio Charlys da Costa Adriana Luchs Flávio Augusto de Pádua Milagres Shirley Vasconcelos Komninakis Danielle Elise Gill Márcia Cristina Alves Brito Sayão Lobato Rafael Brustulin Rogério Togisaki das Chagas Maria de Fátima Neves dos Santos Abrão Cassia Vitória de Deus Alves Soares Xutao Deng Ester Cerdeira Sabino Eric Delwart Élcio Leal Recombination Located over 2A-2B Junction Ribosome Frameshifting Region of Saffold Cardiovirus Viruses saffold virus cardiovirus virome picornavirus ribosomal frameshifting GGUUUUU motif RNA-dependent RNA-polymerase |
| title | Recombination Located over 2A-2B Junction Ribosome Frameshifting Region of Saffold Cardiovirus |
| title_full | Recombination Located over 2A-2B Junction Ribosome Frameshifting Region of Saffold Cardiovirus |
| title_fullStr | Recombination Located over 2A-2B Junction Ribosome Frameshifting Region of Saffold Cardiovirus |
| title_full_unstemmed | Recombination Located over 2A-2B Junction Ribosome Frameshifting Region of Saffold Cardiovirus |
| title_short | Recombination Located over 2A-2B Junction Ribosome Frameshifting Region of Saffold Cardiovirus |
| title_sort | recombination located over 2a 2b junction ribosome frameshifting region of saffold cardiovirus |
| topic | saffold virus cardiovirus virome picornavirus ribosomal frameshifting GGUUUUU motif RNA-dependent RNA-polymerase |
| url | http://www.mdpi.com/1999-4915/10/10/520 |
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