Expression of KNUCKLES in the Stem Cell Domain Is Required for Its Function in the Control of Floral Meristem Activity in Arabidopsis
In the model plant Arabidopsis thaliana, the zinc-finger transcription factor KNUCKLES (KNU) plays an important role in the termination of floral meristem activity, a process that is crucial for preventing the overgrowth of flowers. The KNU gene is activated in floral meristems by the floral organ i...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-07-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2021.704351/full |
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| author | Kamila Kwaśniewska Caoilfhionn Breathnach Christina Fitzsimons Kevin Goslin Bennett Thomson Joseph Beegan Andrea Finocchio Nathanaël Prunet Diarmuid S. Ó’Maoiléidigh Diarmuid S. Ó’Maoiléidigh Frank Wellmer |
| author_facet | Kamila Kwaśniewska Caoilfhionn Breathnach Christina Fitzsimons Kevin Goslin Bennett Thomson Joseph Beegan Andrea Finocchio Nathanaël Prunet Diarmuid S. Ó’Maoiléidigh Diarmuid S. Ó’Maoiléidigh Frank Wellmer |
| author_sort | Kamila Kwaśniewska |
| collection | DOAJ |
| description | In the model plant Arabidopsis thaliana, the zinc-finger transcription factor KNUCKLES (KNU) plays an important role in the termination of floral meristem activity, a process that is crucial for preventing the overgrowth of flowers. The KNU gene is activated in floral meristems by the floral organ identity factor AGAMOUS (AG), and it has been shown that both AG and KNU act in floral meristem control by directly repressing the stem cell regulator WUSCHEL (WUS), which leads to a loss of stem cell activity. When we re-examined the expression pattern of KNU in floral meristems, we found that KNU is expressed throughout the center of floral meristems, which includes, but is considerably broader than the WUS expression domain. We therefore hypothesized that KNU may have additional functions in the control of floral meristem activity. To test this, we employed a gene perturbation approach and knocked down KNU activity at different times and in different domains of the floral meristem. In these experiments we found that early expression in the stem cell domain, which is characterized by the expression of the key meristem regulatory gene CLAVATA3 (CLV3), is crucial for the establishment of KNU expression. The results of additional genetic and molecular analyses suggest that KNU represses floral meristem activity to a large extent by acting on CLV3. Thus, KNU might need to suppress the expression of several meristem regulators to terminate floral meristem activity efficiently. |
| first_indexed | 2024-12-19T10:03:27Z |
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| id | doaj.art-a631ba99a9874f2eba3e48b5e99c4fbb |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-19T10:03:27Z |
| publishDate | 2021-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-a631ba99a9874f2eba3e48b5e99c4fbb2022-12-21T20:26:34ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-07-011210.3389/fpls.2021.704351704351Expression of KNUCKLES in the Stem Cell Domain Is Required for Its Function in the Control of Floral Meristem Activity in ArabidopsisKamila Kwaśniewska0Caoilfhionn Breathnach1Christina Fitzsimons2Kevin Goslin3Bennett Thomson4Joseph Beegan5Andrea Finocchio6Nathanaël Prunet7Diarmuid S. Ó’Maoiléidigh8Diarmuid S. Ó’Maoiléidigh9Frank Wellmer10Smurfit Institute of Genetics, Trinity College Dublin, Dublin, IrelandSmurfit Institute of Genetics, Trinity College Dublin, Dublin, IrelandSmurfit Institute of Genetics, Trinity College Dublin, Dublin, IrelandSmurfit Institute of Genetics, Trinity College Dublin, Dublin, IrelandSmurfit Institute of Genetics, Trinity College Dublin, Dublin, IrelandSmurfit Institute of Genetics, Trinity College Dublin, Dublin, IrelandSmurfit Institute of Genetics, Trinity College Dublin, Dublin, IrelandDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA, United StatesSmurfit Institute of Genetics, Trinity College Dublin, Dublin, IrelandInstitute of Integrative Biology, University of Liverpool, Liverpool, United KingdomSmurfit Institute of Genetics, Trinity College Dublin, Dublin, IrelandIn the model plant Arabidopsis thaliana, the zinc-finger transcription factor KNUCKLES (KNU) plays an important role in the termination of floral meristem activity, a process that is crucial for preventing the overgrowth of flowers. The KNU gene is activated in floral meristems by the floral organ identity factor AGAMOUS (AG), and it has been shown that both AG and KNU act in floral meristem control by directly repressing the stem cell regulator WUSCHEL (WUS), which leads to a loss of stem cell activity. When we re-examined the expression pattern of KNU in floral meristems, we found that KNU is expressed throughout the center of floral meristems, which includes, but is considerably broader than the WUS expression domain. We therefore hypothesized that KNU may have additional functions in the control of floral meristem activity. To test this, we employed a gene perturbation approach and knocked down KNU activity at different times and in different domains of the floral meristem. In these experiments we found that early expression in the stem cell domain, which is characterized by the expression of the key meristem regulatory gene CLAVATA3 (CLV3), is crucial for the establishment of KNU expression. The results of additional genetic and molecular analyses suggest that KNU represses floral meristem activity to a large extent by acting on CLV3. Thus, KNU might need to suppress the expression of several meristem regulators to terminate floral meristem activity efficiently.https://www.frontiersin.org/articles/10.3389/fpls.2021.704351/fullstem cellsfloral meristemmeristem terminationflower developmenttranscription factor |
| spellingShingle | Kamila Kwaśniewska Caoilfhionn Breathnach Christina Fitzsimons Kevin Goslin Bennett Thomson Joseph Beegan Andrea Finocchio Nathanaël Prunet Diarmuid S. Ó’Maoiléidigh Diarmuid S. Ó’Maoiléidigh Frank Wellmer Expression of KNUCKLES in the Stem Cell Domain Is Required for Its Function in the Control of Floral Meristem Activity in Arabidopsis Frontiers in Plant Science stem cells floral meristem meristem termination flower development transcription factor |
| title | Expression of KNUCKLES in the Stem Cell Domain Is Required for Its Function in the Control of Floral Meristem Activity in Arabidopsis |
| title_full | Expression of KNUCKLES in the Stem Cell Domain Is Required for Its Function in the Control of Floral Meristem Activity in Arabidopsis |
| title_fullStr | Expression of KNUCKLES in the Stem Cell Domain Is Required for Its Function in the Control of Floral Meristem Activity in Arabidopsis |
| title_full_unstemmed | Expression of KNUCKLES in the Stem Cell Domain Is Required for Its Function in the Control of Floral Meristem Activity in Arabidopsis |
| title_short | Expression of KNUCKLES in the Stem Cell Domain Is Required for Its Function in the Control of Floral Meristem Activity in Arabidopsis |
| title_sort | expression of knuckles in the stem cell domain is required for its function in the control of floral meristem activity in arabidopsis |
| topic | stem cells floral meristem meristem termination flower development transcription factor |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2021.704351/full |
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