A Comprehensive Guide for Performing Sample Preparation and Top-Down Protein Analysis

A Comprehensive Guide for Performing Sample Preparation and Top-Down Protein Analysis

Methodologies for the global analysis of proteins in a sample, or proteome analysis, have been available since 1975 when Patrick O′Farrell published the first paper describing two-dimensional gel electrophoresis (2D-PAGE). This technique allowed the resolution of single protein isoforms, or proteofo...

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Bibliographic Details
Main Authors: Matthew P. Padula, Iain J. Berry, Matthew B. O′Rourke, Benjamin B.A. Raymond, Jerran Santos, Steven P. Djordjevic
Format: Article
Language:English
Published: MDPI AG 2017-04-01
Series:Proteomes
Subjects:
Proteomics
Top-Down
Bottom-up
Mass spectrometry
Electrophoresis
Isoelectric focusing
Proteoform
Chromatography
Online Access:http://www.mdpi.com/2227-7382/5/2/11

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