Decreased response to acetylcholine during aging of aplysia neuron R15.
How aging affects the communication between neurons is poorly understood. To address this question, we have studied the electrophysiological properties of identified neuron R15 of the marine mollusk Aplysia californica. R15 is a bursting neuron in the abdominal ganglia of the central nervous system...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3874043?pdf=render |
| _version_ | 1811296273877172224 |
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| author | Komolitdin Akhmedov Valerio Rizzo Beena M Kadakkuzha Christopher J Carter Neil S Magoski Thomas R Capo Sathyanarayanan V Puthanveettil |
| author_facet | Komolitdin Akhmedov Valerio Rizzo Beena M Kadakkuzha Christopher J Carter Neil S Magoski Thomas R Capo Sathyanarayanan V Puthanveettil |
| author_sort | Komolitdin Akhmedov |
| collection | DOAJ |
| description | How aging affects the communication between neurons is poorly understood. To address this question, we have studied the electrophysiological properties of identified neuron R15 of the marine mollusk Aplysia californica. R15 is a bursting neuron in the abdominal ganglia of the central nervous system and is implicated in reproduction, water balance, and heart function. Exposure to acetylcholine (ACh) causes an increase in R15 burst firing. Whole-cell recordings of R15 in the intact ganglia dissected from mature and old Aplysia showed specific changes in burst firing and properties of action potentials induced by ACh. We found that while there were no significant changes in resting membrane potential and latency in response to ACh, the burst number and burst duration is altered during aging. The action potential waveform analysis showed that unlike mature neurons, the duration of depolarization and the repolarization amplitude and duration did not change in old neurons in response to ACh. Furthermore, single neuron quantitative analysis of acetylcholine receptors (AChRs) suggested alteration of expression of specific AChRs in R15 neurons during aging. These results suggest a defect in cholinergic transmission during aging of the R15 neuron. |
| first_indexed | 2024-04-13T05:46:49Z |
| format | Article |
| id | doaj.art-a633c525be22430b8698a84dbef52433 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-13T05:46:49Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-a633c525be22430b8698a84dbef524332022-12-22T02:59:56ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01812e8479310.1371/journal.pone.0084793Decreased response to acetylcholine during aging of aplysia neuron R15.Komolitdin AkhmedovValerio RizzoBeena M KadakkuzhaChristopher J CarterNeil S MagoskiThomas R CapoSathyanarayanan V PuthanveettilHow aging affects the communication between neurons is poorly understood. To address this question, we have studied the electrophysiological properties of identified neuron R15 of the marine mollusk Aplysia californica. R15 is a bursting neuron in the abdominal ganglia of the central nervous system and is implicated in reproduction, water balance, and heart function. Exposure to acetylcholine (ACh) causes an increase in R15 burst firing. Whole-cell recordings of R15 in the intact ganglia dissected from mature and old Aplysia showed specific changes in burst firing and properties of action potentials induced by ACh. We found that while there were no significant changes in resting membrane potential and latency in response to ACh, the burst number and burst duration is altered during aging. The action potential waveform analysis showed that unlike mature neurons, the duration of depolarization and the repolarization amplitude and duration did not change in old neurons in response to ACh. Furthermore, single neuron quantitative analysis of acetylcholine receptors (AChRs) suggested alteration of expression of specific AChRs in R15 neurons during aging. These results suggest a defect in cholinergic transmission during aging of the R15 neuron.http://europepmc.org/articles/PMC3874043?pdf=render |
| spellingShingle | Komolitdin Akhmedov Valerio Rizzo Beena M Kadakkuzha Christopher J Carter Neil S Magoski Thomas R Capo Sathyanarayanan V Puthanveettil Decreased response to acetylcholine during aging of aplysia neuron R15. PLoS ONE |
| title | Decreased response to acetylcholine during aging of aplysia neuron R15. |
| title_full | Decreased response to acetylcholine during aging of aplysia neuron R15. |
| title_fullStr | Decreased response to acetylcholine during aging of aplysia neuron R15. |
| title_full_unstemmed | Decreased response to acetylcholine during aging of aplysia neuron R15. |
| title_short | Decreased response to acetylcholine during aging of aplysia neuron R15. |
| title_sort | decreased response to acetylcholine during aging of aplysia neuron r15 |
| url | http://europepmc.org/articles/PMC3874043?pdf=render |
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