A colorimetric/ratiometric chemosensor based on an aggregation-induced emission strategy for tracing hypochlorite in vitro and in vivo
Excessive levels of hypochlorite (ClO−) negatively affect environmental and biological systems. Thus, it is essential to develop sensors that can identify ClO− in various systems such as the environment and living organisms. In this study, we report the development and evaluation of a novel aggregat...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S014765132300458X |
| _version_ | 1797833143797940224 |
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| author | Dongkyun Gil Boeun Choi Jae Jun Lee Hanseul Lee Ki-Tae Kim Cheal Kim |
| author_facet | Dongkyun Gil Boeun Choi Jae Jun Lee Hanseul Lee Ki-Tae Kim Cheal Kim |
| author_sort | Dongkyun Gil |
| collection | DOAJ |
| description | Excessive levels of hypochlorite (ClO−) negatively affect environmental and biological systems. Thus, it is essential to develop sensors that can identify ClO− in various systems such as the environment and living organisms. In this study, we report the development and evaluation of a novel aggregation-induced emission (AIE) strategy-based colorimetric and ratiometric fluorescent chemosensor 2,2′-(((1E,1′E)-[2,2′-bithiophene]− 5,5′-diylbis(methanylylidene))bis(hydrazin-1-yl-2-ylidene))bis(N,N,N-trimethyl-2-oxoethan-1-aminium) chloride (BMH-2∙Cl) for detecting ClO−. BMH-2∙Cl enabled highly selective ClO− detection through a color change from yellow to colorless and a fluorescence color change from turquoise to blue in a perfect aqueous solution. BMH-2∙Cl exhibited low limits of detection (2.4 ×10−6 M for colorimetry and 2.9 ×10−7 M for ratiometric fluorescence) for detecting ClO− with a rapid response within 5 s. The detection mechanism for ClO− and an AIE property change of BMH-2∙Cl were demonstrated by 1H NMR titration, ESI-MS, variation of water fraction (fw) and theoretical calculations. In particular, we confirmed not only the practicality of BMH-2∙Cl by using test strips, but also demonstrated the potential for efficient ClO− detection in biological and environmental systems such as real water samples, living zebrafish and bean sprouts. |
| first_indexed | 2024-04-09T14:19:51Z |
| format | Article |
| id | doaj.art-a6350298c4284fb789e13c7b39f6bbde |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-04-09T14:19:51Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-a6350298c4284fb789e13c7b39f6bbde2023-05-05T04:39:46ZengElsevierEcotoxicology and Environmental Safety0147-65132023-06-01257114954A colorimetric/ratiometric chemosensor based on an aggregation-induced emission strategy for tracing hypochlorite in vitro and in vivoDongkyun Gil0Boeun Choi1Jae Jun Lee2Hanseul Lee3Ki-Tae Kim4Cheal Kim5Department of New and Renewable Energy Convergence and Fine Chemistry, Seoul National University of Science and Technology, Seoul 01811, KoreaDepartment of New and Renewable Energy Convergence and Fine Chemistry, Seoul National University of Science and Technology, Seoul 01811, KoreaDepartment of New and Renewable Energy Convergence and Fine Chemistry, Seoul National University of Science and Technology, Seoul 01811, KoreaDepartment of Environmental Engineering, Seoul National University of Science and Technology, Seoul 01811, KoreaDepartment of Environmental Engineering, Seoul National University of Science and Technology, Seoul 01811, Korea; Corresponding authors.Department of New and Renewable Energy Convergence and Fine Chemistry, Seoul National University of Science and Technology, Seoul 01811, Korea; Corresponding authors.Excessive levels of hypochlorite (ClO−) negatively affect environmental and biological systems. Thus, it is essential to develop sensors that can identify ClO− in various systems such as the environment and living organisms. In this study, we report the development and evaluation of a novel aggregation-induced emission (AIE) strategy-based colorimetric and ratiometric fluorescent chemosensor 2,2′-(((1E,1′E)-[2,2′-bithiophene]− 5,5′-diylbis(methanylylidene))bis(hydrazin-1-yl-2-ylidene))bis(N,N,N-trimethyl-2-oxoethan-1-aminium) chloride (BMH-2∙Cl) for detecting ClO−. BMH-2∙Cl enabled highly selective ClO− detection through a color change from yellow to colorless and a fluorescence color change from turquoise to blue in a perfect aqueous solution. BMH-2∙Cl exhibited low limits of detection (2.4 ×10−6 M for colorimetry and 2.9 ×10−7 M for ratiometric fluorescence) for detecting ClO− with a rapid response within 5 s. The detection mechanism for ClO− and an AIE property change of BMH-2∙Cl were demonstrated by 1H NMR titration, ESI-MS, variation of water fraction (fw) and theoretical calculations. In particular, we confirmed not only the practicality of BMH-2∙Cl by using test strips, but also demonstrated the potential for efficient ClO− detection in biological and environmental systems such as real water samples, living zebrafish and bean sprouts.http://www.sciencedirect.com/science/article/pii/S014765132300458XHypochloriteRatiometric/colorimetric chemosensorAggregation-induced emissionTheoretical calculationBioimagingPlant system |
| spellingShingle | Dongkyun Gil Boeun Choi Jae Jun Lee Hanseul Lee Ki-Tae Kim Cheal Kim A colorimetric/ratiometric chemosensor based on an aggregation-induced emission strategy for tracing hypochlorite in vitro and in vivo Ecotoxicology and Environmental Safety Hypochlorite Ratiometric/colorimetric chemosensor Aggregation-induced emission Theoretical calculation Bioimaging Plant system |
| title | A colorimetric/ratiometric chemosensor based on an aggregation-induced emission strategy for tracing hypochlorite in vitro and in vivo |
| title_full | A colorimetric/ratiometric chemosensor based on an aggregation-induced emission strategy for tracing hypochlorite in vitro and in vivo |
| title_fullStr | A colorimetric/ratiometric chemosensor based on an aggregation-induced emission strategy for tracing hypochlorite in vitro and in vivo |
| title_full_unstemmed | A colorimetric/ratiometric chemosensor based on an aggregation-induced emission strategy for tracing hypochlorite in vitro and in vivo |
| title_short | A colorimetric/ratiometric chemosensor based on an aggregation-induced emission strategy for tracing hypochlorite in vitro and in vivo |
| title_sort | colorimetric ratiometric chemosensor based on an aggregation induced emission strategy for tracing hypochlorite in vitro and in vivo |
| topic | Hypochlorite Ratiometric/colorimetric chemosensor Aggregation-induced emission Theoretical calculation Bioimaging Plant system |
| url | http://www.sciencedirect.com/science/article/pii/S014765132300458X |
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